植物生长调节剂(PGRs)在体外诱导形态发生中起着至关重要的作用。合成PGR通常用于从各种外植体诱导器官发生和体细胞胚发生。而天然物质很少被利用。本研究旨在使用Tulsi(Ocullsancum)叶提取物增强烟草叶外植体的再生反应,并阐明内源性植物生长调节剂调节过程中的生化相互作用,包括吲哚-3-乙酸(IAA),脱落酸(ABA),zeatin,和6-(γ,γ-二甲基烯丙基氨基)嘌呤(2iP)。Tulsi叶提取物通过内源激素与提取物中存在的激素之间的相互作用显着改善了芽的产生,这增强了缓解压力的能力。20%Tulsi叶提取物处理产生的芽明显多于对照,从培养的第10天开始,内源性IAA和玉米素水平增加。此外,ABA和玉米素浓度分别在第15天和第25天增加,在20%的Tulsi提取物治疗中,表明它们在诱导体细胞胚样结构中的作用。ABA可能作为应激反应的激活剂,鼓励这些结构的发展。此外,2iP参与诱导两种形式的再生在10%和20%的提取物处理,特别是与ABA结合。这些结果表明,Tulsi叶提取物具有作为增加体外植物再生和促进我们对如何利用植物来源的天然提取物来优化体外植物再生过程的理解的天然补充剂的潜力。
Plant growth regulators (PGRs) play a vital role in the induction of morphogenesis in vitro. Synthetic PGRs are commonly used to induce organogenesis and somatic embryogenesis from various explants, while natural substances are rarely utilized. This study aimed to enhance the regenerative response in Nicotiana tabacum leaf explants using Tulsi (Ocimum sanctum) leaf extract and to elucidate the biochemical interactions during modulation of endogenous plant growth regulators, including indole-3-acetic acid (IAA), abscisic acid (ABA), zeatin, and 6-(γ, γ-dimethylallylamino) purine (2iP). Tulsi leaf extract significantly improved shoot production through interactions between endogenous hormones and those present in the extract, which enhanced stress mitigation. The 20% Tulsi leaf extract treatment produced significantly more shoots than the control, coinciding with increased endogenous IAA and zeatin levels starting on day 10 in culture. Furthermore, ABA and zeatin concentrations increased on days 15 and 25, respectively, in the 20% Tulsi extract treatment, suggesting their role in the induction of somatic embryo-like structures. ABA likely acts as an activator of stress responses, encouraging the development of these structures. Additionally, 2iP was involved in the induction of both forms of regeneration in the 10% and 20% extract treatments, especially in combination with ABA. These results suggest that Tulsi leaf extract holds promising potential as a natural supplement for increasing plant regeneration in vitro and advancing our understanding of how natural extracts of plant origin can be harnessed to optimize plant regeneration processes in vitro.