PDF(Pubmed)
Abstract：
The medicinal plant tulsi (Ocimum sanctum L.) is acknowledged for its invigorating and healing properties that enhance resilience to stress in various human and animal models by modulating antioxidant compounds. While extensive research has documented these effects in humans, the adaptogenic potential of tulsi in stressful in vitro plant systems has not been explored. This study aimed to elucidate the adaptogenic properties of tulsi leaf extract on the in vitro regeneration of tobacco leaf explants through an investigation of the indoleamines at different developmental stages. Shoot regeneration from leaf explants on the medium supplemented with tulsi extract (20%) was compared to the control, and the differences in indoleamine compounds were analyzed using ultra-performance liquid chromatography. Treatment of the explants with the extract resulted in an almost two-fold increase in the number of regenerants after four weeks of culture, and 9% of the regenerants resembled somatic embryo-like structures. The occurrence of browning in the extract-treated explants stopped on day 10, shoots began to develop, and a significant concentration of tryptamine and N-acetyl-serotonin accumulated. A comparative analysis of indoleamine compounds in intact and cut tobacco leaves also revealed the pivotal role of melatonin and 2-hydroxymelatonin functioning as antioxidants during stress adaptation. This study demonstrates that tulsi is a potent adaptogen that is capable of modulating plant morphogenesis in vitro, paving the way for further investigations into the role of adaptogens in plant stress biology.
摘要：
药用植物tulsi（OcimumsanctumL.）因其振兴和愈合特性而闻名，该特性通过调节抗氧化剂化合物来增强各种人类和动物模型的抗逆性。虽然广泛的研究已经记录了这些对人类的影响，尚未探索tulsi在应激体外植物系统中的适应基因潜力。本研究旨在通过研究不同发育阶段的吲哚胺，阐明tulsi叶提取物对烟草外植体体外再生的适应性。将补充有tulsi提取物（20％）的培养基上的叶外植体的芽再生与对照进行比较，并使用超高效液相色谱法分析吲哚胺化合物的差异。用提取物处理外植体导致在培养四周后再生体的数量增加了几乎两倍。9%的再生体类似于体细胞胚状结构。在第10天停止的提取物处理的外植体褐变的发生，芽开始发展，和显著浓度的色胺和N-乙酰血清素积累。对完整和切割的烟叶中吲哚胺化合物的比较分析也揭示了褪黑激素和2-羟基褪黑激素在应激适应过程中作为抗氧化剂的关键作用。这项研究表明，tulsi是一种有效的适应原，能够在体外调节植物形态发生，为进一步研究适应原在植物胁迫生物学中的作用铺平了道路。
