背景:乌兹别克斯坦是与饮食相关的慢性疾病数量最多的国家之一,这被认为与高动物脂肪摄入量有关。绵羊肉富含脂肪(肌肉中脂肪含量约为5%),包括饱和和单不饱和脂肪酸,与牛肉相比,它含有的n-3多不饱和脂肪酸和共轭亚油酸的含量几乎是牛肉的两倍。然而,羊肉被乌兹别克斯坦当地人认为是促进健康的,它约占该国红肉摄入量的1/3。
目的:本研究的目的是应用代谢组学方法来调查健康乌兹别克成年人的绵羊肉摄入频率(SMIF)是否与空腹血浆代谢产物和脂蛋白的改变有关。
方法:该研究包括263名受试者,149名女性和114名男性。为每个受试者提供食物摄入问卷,包括SMIF,记录并收集空腹血浆样品用于代谢组学。使用1HNMR光谱法测定血浆代谢物和脂蛋白浓度。
结论:结果表明,SMIF受到国籍的混淆,性别,体重指数(BMI),年龄,总肉类和鱼类的摄入频率按升序排列(p<0.01)。多变量和单变量数据分析显示血浆代谢物和脂蛋白水平相对于SMIF的差异。按国籍统计调整后的SMIF效果,性别,BMI,年龄,总肉类和鱼类的摄入频率下降,但仍然显著。丙酮酸,苯丙氨酸,鸟氨酸,在高SMIF组中,乙酸仍然显着降低,而胆碱,天冬酰胺,二甲基甘氨酸呈上升趋势。胆固醇水平,载脂蛋白A1以及低密度和高密度脂蛋白亚组分均显示出随着SMIF增加而下降的趋势,尽管FDR校正后差异不显著。
Uzbekistan is one of the countries with the highest number of diet-related chronic diseases, which is believed to be associated with high animal fat intake. Sheep meat is high in fats (~ 5% in muscle), including saturated and monounsaturated fatty acids, and it contains nearly twice the higher amounts of n-3 polyunsaturated fatty acids and conjugated linoleic acids compared to beef. Nevertheless, sheep meat is considered health promoting by the locals in Uzbekistan and it accounts for around 1/3 of red meat intake in the country.
The aim of this study was to apply a metabolomics approach to investigate if sheep meat intake frequency (SMIF) is associated with alterations in fasting blood plasma metabolites and lipoproteins in healthy Uzbek adults.
The study included 263 subjects, 149 females and 114 males. For each subject a food intake questionnaire, including SMIF, was recorded and fasting blood plasma samples were collected for metabolomics. Blood plasma metabolites and lipoprotein concentrations were determined using 1H NMR spectroscopy.
The results showed that SMIF was confounded by nationality, sex, body mass index (BMI), age, intake frequency of total meat and fish in ascending order (p < 0.01). Multivariate and univariate data analyses showed differences in the levels of plasma metabolites and lipoproteins with respect to SMIF. The effect of SMIF after statistical adjustment by nationality, sex, BMI, age, intake frequency of total meat and fish decreased but remained significant. Pyruvic acid, phenylalanine, ornithine, and acetic acid remained significantly lower in the high SMIF group, whereas choline, asparagine, and dimethylglycine showed an increasing trend. Levels of cholesterol, apolipoprotein A1, as well as low- and high-density lipoprotein subfractions all displayed a decreasing trend with increased SMIF although the difference were not significant after FDR correction.