PDF(Pubmed)
Abstract：
Sarcocystis spp. are complex apicomplexan parasites that cause a substantial economic impact on livestock used for meat production. These parasites are present worldwide. Our study aimed to identify Sarcocystis species affecting sheep meat in southern-central Spain and to evaluate the effectiveness of freezing for parasite inactivation. A total of 210 condemned samples of sheep meat were thoroughly assessed grossly and microscopically; the presence of macro- and microcysts was confirmed. The samples were then frozen at -20 °C for various time intervals (24, 48, 72, 96, 120, and 144 h) and compared with untreated samples. Bradyzoites were isolated through pepsin digestion for subsequent molecular analysis and viability assessment, employing trypan blue and double fluorescence staining techniques. Our measurements confirmed the presence of S. tenella, S. gigantea, and S. medusiformis in Spanish domestic sheep. Freezing for 96 to 144 h resulted in a significant reduction in parasite viability, with a robust correlation observed between the two staining methods. Both stains effectively measured the viability of Sarcocystis, thereby promising future advances in meat safety.
摘要：
结节虫。是复杂的顶孔虫寄生虫，对用于肉类生产的牲畜造成重大的经济影响。这些寄生虫存在于世界各地。我们的研究旨在确定西班牙中南部影响绵羊肉的肉孢子虫物种，并评估冷冻对寄生虫灭活的有效性。对总共210个谴责的绵羊肉样品进行了全面和显微镜彻底评估；确认了大囊肿和小囊肿的存在。然后将样品在-20°C下冷冻各种时间间隔(24、48、72、96、120和144h),并与未处理的样品进行比较。通过胃蛋白酶消化分离出缓生子，用于随后的分子分析和活力评估。采用台盼蓝和双荧光染色技术。我们的测量证实了丝虫的存在，S.gigantea，和西班牙家养绵羊的S.medusiformis。冷冻96至144小时导致寄生虫生存能力显着降低，在两种染色方法之间观察到强烈的相关性。两种染色都有效地测量了结节虫的生存力，从而有望在肉类安全方面取得未来进展。
