■累积证据表明,乳腺癌是女性最常见的癌症。尽管越来越多的证据表明血清素和肿瘤发生之间存在联系,关于人乳腺癌细胞和组织中5-羟色胺能系统表达的研究很少有报道。
■首先,免疫荧光染色,ELISA和Western印迹用于检测各种乳腺癌细胞类型中的5-羟色胺和褪黑激素能系统。然后,使用免疫荧光测定在TPBC细胞系BT-474和TNBC细胞系MDA-MB-231的培养物中评估血清素的表达。进一步探讨5-羟色胺在乳腺癌诊断中的作用,通过免疫染色分析在TPBC和TNBC肿瘤切片中进行血清素表达。
■我们的结果表明,人乳腺癌细胞和人乳腺上皮细胞系都可以合成5-羟色胺和褪黑素。不像褪黑激素,5-羟色胺水平在人乳腺癌和乳腺上皮细胞系之间存在显着差异(p<0.01)。此外,5-羟色胺N-乙酰转移酶(NAT)和乙酰5-羟色胺甲基转移酶(ASMT),从5-羟色胺合成褪黑素途径中的关键酶,也可以检测到。与人乳腺癌细胞和人乳腺上皮细胞系的这些发现一致,三阴性血清素表达也高得多(PR-,ER-,HER-2-)乳腺癌(TNBC)和三阳性乳腺癌(TPBC)与癌旁组织(PCT)相比。
■这里,我们提供的证据表明,人乳腺癌细胞(MCF-7,Bcap-37)和人乳腺上皮细胞(MCF-10A)可以合成内在的5-羟色胺和褪黑激素,与PCT相比,乳腺癌组织中5-羟色胺的表达更高。研究结果表明,血清素可能被用作乳腺癌患者的预测指标。
UNASSIGNED: Cumulative evidence has demonstrated that breast cancer was the most commonly diagnosed cancer in women. Despite growing evidence for a link between serotonin and tumorigenesis, research on the expression of serotoninergic systems in the human breast cancer cell and tissue has only rarely been reported.
UNASSIGNED: First, immunofluorescence staining, ELISA and Western blotting were used to detect serotonin and melatoninergic systems in various breast cancer cell types. Then, serotonin expression was evaluated in the cultures of TPBC cell line BT-474 and TNBC cell line MDA-MB-231 using immunofluorescence assay. To further explore the diagnostic role of serotonin in breast cancer, serotonin expression was conducted in the TPBC and TNBC tumor sections by immunostaining analysis.
UNASSIGNED: Our results suggested that both human breast cancer cells and human breast epithelial cell line could synthesize serotonin and melatonin. Unlike melatonin, serotonin levels varied significantly between human breast cancer and breast epithelial cell line (p<0.01). In addition, serotonin N-acetyltransferase (NAT) and acetylserotonin methyltransferase (ASMT), the key enzymes in the pathway of melatonin synthesis from serotonin, were also detectable. In agreement with these findings of human breast cancer cell and human breast epithelial cell line, serotonin expression was also much higher in triple-negative (PR-, ER-, HER-2-) breast cancer (TNBC) and triple-positive breast cancer (TPBC) compared to para-carcinoma tissues (PCTs).
UNASSIGNED: Here, we provided evidence that the human breast cancer cell (MCF-7, Bcap-37) and human breast epithelial cell (MCF-10A) could synthesize intrinsic serotonin and melatonin, and serotonin expression was higher in the breast cancer tissue compared with PCT. The findings suggested that serotonin might be used as a predictive marker for breast cancer patients.