关键词： MB3 MG149 histone acetyltransferase inhibitor melatonin rice serotonin N-acetyltransferase

Mesh ： Arylalkylamine N-Acetyltransferase / antagonists & inhibitors genetics Cadmium / pharmacology Dose-Response Relationship, Drug Melatonin / metabolism Oryza / genetics metabolism Plant Proteins / antagonists & inhibitors genetics Recombinant Proteins / metabolism Salicylates / pharmacology Seedlings / genetics metabolism Serotonin / analogs & derivatives metabolism

来  源：   DOI：10.3390/biom11050658   PDF(Sci-hub)   PDF(Pubmed)

Abstract：
We examined the effects of two histone acetyltransferase (HAT) inhibitors on the activity of rice serotonin N-acetyltransferases (SNAT). Two rice recombinant SNAT isoenzymes (SNAT1 and SNAT2) were incubated in the presence of either MG149 or MB3, HAT inhibitors. MG149 significantly inhibited the SNAT enzymes in a dose-dependent manner, especially SNAT1, while SNAT2 was moderately inhibited. By contrast, MB3 had no effect on SNAT1 or SNAT2. The application of 100 μM MG149 to rice seedlings decreased melatonin by 1.6-fold compared to the control, whereas MB3 treatment did not alter the melatonin level. MG149 significantly decreased both melatonin and N-acetylserotonin when rice seedlings were challenged with cadmium, a potent elicitor of melatonin synthesis in rice. Although MG149 inhibited melatonin synthesis in rice seedlings, no melatonin deficiency-induced lamina angle decrease was observed due to the insufficient suppression of SNAT2, which is responsible for the lamina angle decrease in rice.
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