■对于大多数暴躁症患者,血清学是诊断的基础。这项研究的目的是对在瑞典参考实验室分析的样品进行分析,以确定怀疑患有tularemia的个体的血清中是否存在土拉弗朗西斯菌特异性抗体水平。分析了样本总数和阳性样本比例的年度和月度变化,以及年龄和性别的影响。
■我们对在2010年至2022年期间在瑞典于默奥大学医院分析的疑似tularemia患者的血清学样本中是否存在tularensis特异性抗体进行了回顾性分析,国家参考实验室,通过使用各种统计方法。总的来说,在13年期间,我们通过ELISA分析了约15,100份血清样本中IgG和IgM抗体的存在.
■总的来说,IgG阳性或临界滴度的样本数量较多,分别为2,522和921,比IgM阳性或临界滴度高,分别为1,802和409。从大约1,930名个体和大约三分之一的病例中获得了重复样本,最初是血清阴性的,重新取样时已血清转化。8月和9月的每月样本数量最高,>3,000。年度数量差异很大,2015年和2019年观察到峰值数量分别为1,832和2,250,而其他年份的数字是700-800。阳性样本的年度和月度百分比也有很大差异,它们在小于10%到大于20%之间变化。阳性样本的百分比最高的是在9月和10月。IgG和IgM滴度随着年龄的增长而下降,这些差异对于IgG滴度非常显着,每20年间隔平均滴度降低。
■集体,数据表明,瑞典的兔热病采样存在显著的年度和季节性变化.此外,阳性样本的比例在数月和数年内增加,样本数量达到峰值。另一个值得注意的发现是平均抗体滴度随着年龄的增加而降低。
UNASSIGNED: For a majority of tularemia patients, serology is the basis for the diagnosis. The aim of this study was to perform an analysis of the samples analyzed at a Swedish reference laboratory for the presence of Francisella tularensis-specific antibody levels in sera from individuals with suspected tularemia. Annual and monthly variations of the total number of samples and proportions of positive samples were analyzed, as well as the influence of age and gender.
UNASSIGNED: We performed a retrospective analysis of the presence of F. tularensis-specific antibodies in serological samples from patients with suspected tularemia analyzed during the period 2010 - 2022 at the University Hospital of Umeå in Sweden, a national reference laboratory, by use of various statistical methods. In total, some 15,100 serum samples had been analyzed for the presence of IgG and IgM antibodies by ELISA during the 13-year period.
UNASSIGNED: Overall, there were higher number of samples with IgG positive or borderline titers, 2,522 and 921, respectively, than with IgM positive or borderline titers, 1,802 and 409, respectively. Repeated samples were obtained from some 1,930 individuals and approximately a third of the cases, which were initially seronegative, had seroconverted when resampled. Peak number of monthly samples were recorded in August and September, > 3,000. Annual numbers varied greatly and peak numbers were observed in 2015 and 2019, 1,832 and 2,250, respectively, whereas some other years the numbers were 700 - 800. There was also much variation in the annual and monthly percentages of positive samples and they varied between less than 10% to greater than 20%. The highest percentages of positive samples were recorded in September and October. IgG and IgM titers declined with age and these differences were highly significant for IgG titers, with decreasing average titers for each 20-year interval.
UNASSIGNED: Collectively, the data demonstrate the marked annual and seasonal variations in tularemia sampling occurring in Sweden. Also, the proportion of positive samples increased during months and years with peak number of samples. Another notable finding was that average antibody titers decreased with increased age.