serological diagnosis

血清学诊断
  • 文章类型: Journal Article
    牛短暂性热病毒(BEFV)是弹状病毒科中的一种病毒属。它是一种节肢动物传播的病毒,由许多蚊子和蚊子传播。由于牛奶生产的损失以及牛和水牛的一般状况,它可能会造成严重的经济后果。BEF发生在一些热带,非洲的亚热带和暖温带地区,澳大利亚,中东和亚洲有季节性爆发,但不能排除其可能传播到其他地区(例如欧洲)。因此,使用和开发具有最佳性能的快速诊断方法对于识别新出现的病原体及其控制至关重要。在本研究中,我们开发了两种基于单克隆抗体(mAb)的竞争性血清学ELISA,用BEF灭活病毒抗原和BEF重组核蛋白(N)设计,分别。一组77个BEF阳性和338个BEF阴性血清用于评估两种测试。使用灭活病毒的诊断灵敏度为97.4%,使用重组N的诊断灵敏度为98.7%,使用两种抗原的诊断特异性为100%,我们的结果表明,这些测试适用于BEF的血清学诊断。
    Bovine ephemeral fever virus (BEFV) is a member of the genus Ephemerovirus in the family Rhabdoviridae. It is an arthropod-borne virus transmitted by many species of midges and mosquitoes. It can cause severe economic consequences due to losses in milk production and the general condition of cattle and water buffalo. BEF occurs in some tropical, subtropical and warm temperate regions of Africa, Australia, the Middle East and Asia with seasonal outbreaks, but its possible spread to other areas (e.g. Europe) cannot be excluded. Therefore, using and developing rapid diagnostic methods with optimal performance is essential for identifying emerging pathogens and their control. In the present study, we developed two competitive serological ELISAs based on monoclonal antibodies (mAbs), designed by using BEFV inactivated antigen and the BEF recombinant nucleoprotein (N), respectively. A panel of 77 BEF-positive and 338 BEF-negative sera was used to evaluate the two tests. With a diagnostic sensitivity of 97.4 % using the inactivated virus and 98.7 % using the recombinant N, and a diagnostic specificity of 100 % using both antigens, our results suggest that these tests are suitable for the serological diagnosis of BEF.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

    求助全文

  • 文章类型: Journal Article
    严重发热伴血小板减少综合征病毒(SFTSV)或大别包带病毒是引起SFTS的新兴病原体。它被认为是对人类健康的新威胁,考虑到高相关死亡率。SFTSV是一种分段负链RNA病毒,含有三个单链RNA,具有编码糖蛋白Gn和Gc的M区段。GC对于病毒进入宿主细胞表面至关重要,还有Gn蛋白.由于Gc是病毒粒子的表面暴露抗原,它是感染的关键诊断标记。尽管已经开发了各种基于SFTSVGn或N蛋白的血清诊断方法,没有市售的血清诊断试剂盒。因此,我们产生了抗SFTSVGc的单克隆抗体(mAb),并探索了它们在血清诊断试验中的应用,以开发涵盖广泛基因型(A至F)的敏感血清诊断工具.首先,使用噬菌体展示系统分离SFTSVGc抗体结合片段(Fab)并转化为人IgG。SFTSV和裂谷热病毒(RVFV:与SFTSV相同属)Gc抗原的酶联免疫吸附测定(ELISA)表明,与SFTSVGc蛋白连接的所有抗体均具有高亲和力。免疫荧光分析(IFA),为了验证七种对各种SFTSV基因型具有高亲和力的抗体的交叉反应性(A,B2、B3、D、和F)并检测mAb与完整的Gc蛋白的结合,显示五种IgG型mAb与各种基因型的完整Gc蛋白结合。使用ELISA和IFA选择六种高亲和力抗体。使用表面等离子体共振测量6种抗体针对SFTSVGc抗原的结合能力。所有抗体都具有高结合能力。因此,这些抗体在SFTSV的血清学诊断中作为有价值的标志物。
    Severe fever with thrombocytopenia syndrome virus (SFTSV) or Dabie bandavirus is an emerging pathogen responsible for SFTS. It is considered a novel threat to human health, given the high associated fatality. SFTSV is a segmented negative-strand RNA virus containing three single-stranded RNAs, with the M segment encoding the glycoproteins Gn and Gc. Gc is vital for viral entry into the host cell surface, along with the Gn protein. As the Gc is the surface-exposable antigen from virions, it is a critical diagnostic marker of infection. Although various SFTSV Gn or N protein-based sero-diagnostic methods have been developed, there are no commercially available sero-diagnostic kits. Therefore, we generated monoclonal antibodies (mAbs) against SFTSV Gc and explored their application in serum diagnostic tests to develop sensitive serodiagnostic tools covering broad-range genotypes (A to F). First, 10 SFTSV Gc antibody-binding fragments (Fabs) were isolated using a phage display system and converted into human IgGs. Enzyme-linked immunosorbent assays (ELISA) of the SFTSV and Rift Valley fever virus (RVFV: same genus as SFTSV) Gc antigens showed that all antibodies attached to the SFTSV Gc protein had high affinity. An immunofluorescence assay (IFA), to verify the cross-reactivity of seven antibodies with high affinities for various SFTSV genotypes (A, B2, B3, D, and F) and detect mAb binding with intact Gc proteins, revealed that five IgG type mAbs were bound to intact Gc proteins of various genotypes. Six high-affinity antibodies were selected using ELISA and IFA. The binding capacity of the six antibodies against the SFTSV Gc antigen was measured using surface plasmon resonance. All antibodies had high binding capacity. Consequently, these antibodies serve as valuable markers in the serological diagnosis of SFTSV.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    目的:本研究旨在建立双抗原夹心ELISA(DAgS-ELISA)方法,准确,并定量检测抗白念珠菌烯醇酶1(CaEno1)的总抗体,以诊断侵袭性念珠菌病(IC)。
    方法:使用重组CaEnol和单克隆抗体作为标准品开发DAgS-ELISA。性能评估包括检测限,准确度,和可重复性。使用DAgS-ELISA分析来自全身性念珠菌病小鼠的血清中抗CaEnol抗体水平的动态变化。来自IC的患者血清样本,念珠菌定植,细菌感染,和健康对照组用DAgS-ELISA和间接ELISA进行分析。
    结果:DAgS-ELISA在线性范围和测试背景方面优于间接ELISA。在全身性念珠菌病小鼠中,在动态抗体水平中观察到独特的“双峰”模式。此外,通过DAgS-ELISA和间接ELISA检测到的CaEno1抗体阳性率具有较高的一致性.虽然患者组的阳性率不同,在各阳性患者组中未检测到抗体水平的显著差异.
    结论:DAgS-ELISA为IC诊断提供了一种可靠的新方法,快速启用,准确,并定量检测CaEno1抗体。其临床应用和有效性需要进一步验证和优化。
    OBJECTIVE: This study aimed to develop a double antigen sandwich ELISA (DAgS-ELISA) method for more efficient, accurate, and quantitative detection of total antibodies against Candida albicans enolase1 (CaEno1) for diagnosing invasive candidiasis (IC).
    METHODS: DAgS-ELISA was developed using recombinant CaEno1 and a monoclonal antibody as the standard. Performance evaluation included limit of detection, accuracy, and repeatability. Dynamic changes in antibody levels against CaEno1 in serum from systemic candidiasis mice were analyzed using DAgS-ELISA. Patient serum samples from IC, Candida colonization, bacterial infections, and healthy controls were analyzed with DAgS-ELISA and indirect ELISA.
    RESULTS: DAgS-ELISA outperformed indirect ELISA in terms of linear range and test background. In systemic candidiasis mice, a distinctive \'double-peak\' pattern in dynamic antibody levels was observed. Additionally, there was a high level of consistency in the positive rates of CaEno1 antibodies detected by both DAgS-ELISA and indirect ELISA. While the positivity rates differed among patient groups, no significant variations in antibody levels were detected among the various positive patient groups.
    CONCLUSIONS: DAgS-ELISA offers a reliable novel approach for IC diagnosis, enabling rapid, accurate, and quantitative detection of CaEno1 antibodies. Further validation and optimization are needed for its clinical application and effectiveness.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

    求助全文

  • 文章类型: Journal Article
    Nipah和Hendra病毒属于副粘病毒科,对人类健康构成重大威胁,零星爆发导致严重的发病率和死亡率。早期症状包括发烧,咳嗽,喉咙痛,头痛,在鉴别诊断方面提供的很少。没有针对这些病毒的特异性治疗剂和疫苗。
    这篇综述全面涵盖了一系列尼帕和亨德拉病毒感染的诊断技术,在感染进展过程中结合适当类型的样本进行讨论。血清学测定,逆转录酶实时PCR检测,和等温扩增试验进行了详细讨论,以及一些市售检测试剂盒的列表。还涵盖了保护Nipah和Hendra病毒检测发明的专利。
    尽管在过去十年中多次爆发Nipah和Hendra感染,深入研究其发病机理,即时诊断,特定疗法,人类缺乏疫苗。及时准确的诊断对于有效的爆发管理至关重要,患者治疗,采取预防措施。快速即时测试的出现有望增强现实环境中的诊断能力。专利格局强调了法律和商业领域内创新和合作的重要性。
    UNASSIGNED: Nipah and Hendra viruses belong to the Paramyxoviridae family, which pose a significant threat to human health, with sporadic outbreaks causing severe morbidity and mortality. Early symptoms include fever, cough, sore throat, and headache, which offer little in terms of differential diagnosis. There are no specific therapeutics and vaccines for these viruses.
    UNASSIGNED: This review comprehensively covers a spectrum of diagnostic techniques for Nipah and Hendra virus infections, discussed in conjunction with appropriate type of samples during the progression of infection. Serological assays, reverse transcriptase Real-Time PCR assays, and isothermal amplification assays are discussed in detail, along with a listing of few commercially available detection kits. Patents protecting inventions in Nipah and Hendra virus detection are also covered.
    UNASSIGNED: Despite several outbreaks of Nipah and Hendra infections in the past decade, in-depth research into their pathogenesis, Point-of-Care diagnostics, specific therapies, and human vaccines is lacking. A prompt and accurate diagnosis is pivotal for efficient outbreak management, patient treatment, and the adoption of preventative measures. The emergence of rapid point-of-care tests holds promise in enhancing diagnostic capabilities in real-world settings. The patent landscape emphasizes the importance of innovation and collaboration within the legal and business realms.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

    求助全文

  • 文章类型: Journal Article
    由于当前诊断方案的敏感性和特异性有限,在巴西诊断犬内脏利什曼病(CVL)面临挑战。因此,迫切需要绘制新抗原或增强现有抗原,以用于未来的诊断技术。免疫信息学工具在鉴定新的潜在表位或抗原候选物中很有希望。在这项研究中,我们在ELISA测定中评估了通过表位预测选择的用于CVL血清诊断的肽。十个B细胞表位在计算机上具有免疫原性,而是两个肽(肽号45号48)在体外表现最好。选择的肽,无论是单独还是组合,在诊断上非常准确,敏感性范围为86.4%至100%,特异性约为90%。我们观察到,与单独的肽相比,肽的组合显示出更好的性能,通过检测所有无症状的狗,在其他犬类感染的狗血清中显示较低的交叉反应性,并且没有检测到接种疫苗的动物。此外,我们的数据表明,与ELISA测定相关的免疫信息学工具可能用于选择和评估潜在的新靶标,如肽,应用于CVL的诊断。
    Diagnosing canine visceral leishmaniasis (CVL) in Brazil faces challenges due to the limitations regarding the sensitivity and specificity of the current diagnostic protocol. Therefore, it is urgent to map new antigens or enhance the existing ones for future diagnostic techniques. Immunoinformatic tools are promising in the identification of new potential epitopes or antigen candidates. In this study, we evaluated peptides selected by epitope prediction for CVL serodiagnosis in ELISA assays. Ten B-cell epitopes were immunogenic in silico, but two peptides (peptides No. 45 and No. 48) showed the best performance in vitro. The selected peptides, both individually and in combination, were highly diagnostically accurate, with sensitivities ranging from 86.4% to 100% and with a specificity of approximately 90%. We observed that the combination of peptides showed better performance when compared to peptide alone, by detecting all asymptomatic dogs, showing lower cross-reactivity in sera from dogs with other canine infections, and did not detect vaccinated animals. Moreover, our data indicate the potential use of immunoinformatic tools associated with ELISA assays for the selection and evaluation of potential new targets, such as peptides, applied to the diagnosis of CVL.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    背景:这项研究的目的是比较天然抗原ELISA和ADAMU-AE/CE商业ICT检测试剂盒在暴露于棘球蚴感染或临床诊断为肺泡(AE)或囊性(CE)棘球蚴病的受试者中的诊断性能。
    方法:招募了来自中国西北部的370名先前有CE或AE临床确认的受试者。在社区调查中,还从3923名儿童/青少年中获得了血清样本。使用天然抗原ELISA测试所有血清。随后将ADAMU-AE/CE检测试剂盒用于370名临床确诊的个体和251名儿童/青少年的血清学,这些儿童/青少年对两种棘球蚴均为ELISA抗体阳性,但在基线调查中超声阴性。对89例AE和164例CE进行了血清学测试与超声分类之间的关联分析。进行Kappa一致性分析以比较天然抗原ELISA和ADAMU试剂盒的诊断性能以及超声成像结果。χ²检验也用于比较组间不同的血清阳性率。
    结果:在病例和被调查儿童/青少年中,用于诊断AE和CE的天然抗原ELISA和ADAMU试剂盒之间的一致性较差(AE和CE分别为Kappa=0.26和0.28)。但ADAMU-AE试剂盒与AE病例的超声观察结果之间的一致性相对较好(Kappa=0.63)。此外,在251名通过天然抗原ELISA对AE和CE抗体均呈共阳性的青少年中,只有一个被ADAMU-AE试剂盒发现阳性,在随后的超声随访中证实为新的AE病例。其余(N=250)通过使用ADAMU-AE/CE试剂盒的血清学和通过超声检查为阴性。两种天然抗原ELISA不能很好地区分临床诊断的AE和CE的病例。相比之下,ADAMU-AE和ADAMU-CE商业ICT测试试剂盒很容易将AE与CE区分开来,AE的特异性为99%,CE的特异性为100%。
    结论:ADAMU-AE/CE试剂盒被证明是可靠的,准确,并在临床环境中适用于确认可疑AE/CE病例的诊断工具。天然抗原ELISA测试可以提供有关人类暴露于棘球蚴感染水平的有用信息。
    BACKGROUND: The aim of this study was to compare the diagnostic performance of native antigen ELISAs and ADAMU-AE/CE commercial ICT test kits in subjects either exposed to Echinococcus infection or with clinically diagnosed alveolar (AE) or cystic (CE) echinococcosis.
    METHODS: A total of 370 subjects with a previous clinical confirmation of CE or AE from northwestern China were recruited. Serum samples were also obtained from 3923 children/teenagers during a community survey. All sera were tested using native antigen ELISAs. The ADAMU-AE/CE test kits were subsequently used for the serology of the 370 clinically confirmed individuals and of 251 children/teenagers that were ELISA antibody-positive for both Echinococcus species but ultrasound-negative during baseline survey. An analysis of the association between the serological tests and ultrasound classification was carried out amongst 89 AE and 164 CE cases. A Kappa consistency analysis was undertaken to compare the diagnostic performance of the native antigen ELISAs and the ADAMU kits and the ultrasound imaging results. The χ² test was also used for a comparison of the different seropositivity rates between the groups.
    RESULTS: There was poor consistency (Kappa = 0.26 and 0.28 for AE and CE respectively) between the native antigen ELISAs and the ADAMU kits for the diagnosis of AE and CE among the cases and the surveyed children/teenagers, but a relatively good consistency (Kappa = 0.63) between the ADAMU-AE kit and ultrasound observations for the AE cases. Additionally, of the 251 teenagers co-positive for both AE and CE antibodies by the native antigen ELISAs, only one was found positive by the ADAMU-AE kit, verified as a new AE case on subsequent ultrasound follow-up. The remainder (N = 250) were negative by serology using the ADAMU-AE/CE kits and by ultrasound examination. The two native antigen ELISAs did not discriminate well between cases of clinically diagnosed AE and CE. In contrast, ADAMU-AE and ADAMU-CE commercial ICT test kits readily differentiated cases of AE from CE with specificities of 99% for AE and 100% for CE.
    CONCLUSIONS: The ADAMU-AE/CE kits proved reliable, accurate, and amenable diagnostic tools in the clinical setting for confirmation of suspected AE/CE cases. The native antigen ELISAs tests can provide useful information on the level of human exposure to Echinococcus infection.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    非洲猪瘟病毒(ASFV)是一种复杂的DNA病毒,是Asfarviridae家族的唯一成员。它导致猪的高死亡率和严重的经济损失。ASFVpB602L蛋白在病毒组装中起关键作用,并且作为主要衣壳蛋白p72的分子伴侣起作用。此外,pB602L是非洲猪瘟(ASF)诊断工具开发的重要靶标,因为它是针对ASFV的高度免疫原性抗原。在这项研究中,我们表达并纯化了ASFVpB602L,并验证了其在ASFV自然感染猪血清中的免疫原性。此外,我们使用杂交瘤技术成功地产生了针对pB602L的IgG2aκ亚类单克隆抗体(mAb7E7)。使用蛋白质印迹和免疫荧光测定,mAb7E7在体外特异性识别ASFVPig/HLJ/2018/株和真核重组ASFVpB602L蛋白。ASFVpB602LC末端的474SKENLTPDE482表位被鉴定为mAb7E7结合的最小线性表位,具有通过蛋白质印迹测定法表征的数十个截短的pB602l片段。我们还表明该抗原表位序列具有较高的保守性和抗原性指数。我们的研究有助于改进疫苗和抗病毒药物的开发,并为ASF的血清学诊断提供了新的见解。关键点:•我们开发了抗ASFVpB602L的单克隆抗体,可以特异性识别ASFV猪/HLJ/2018/株。•本研究发现了一种新的保守B细胞表位474SKENLTPDE482。•在3D结构中,474SKENLTPDE482暴露在ASFVpB602L的表面上,形成弯曲的线性结构。
    African swine fever virus (ASFV) is a complex DNA virus and the only member of the Asfarviridae family. It causes high mortality and severe economic losses in pigs. The ASFV pB602L protein plays a key role in virus assembly and functions as a molecular chaperone of the major capsid protein p72. In addition, pB602L is an important target for the development of diagnostic tools for African swine fever (ASF) because it is a highly immunogenic antigen against ASFV. In this study, we expressed and purified ASFV pB602L and validated its immunogenicity in serum from naturally infected pigs with ASFV. Furthermore, we successfully generated an IgG2a κ subclass monoclonal antibody (mAb 7E7) against pB602L using hybridoma technology. Using western blot and immunofluorescence assays, mAb 7E7 specifically recognized the ASFV Pig/HLJ/2018/strain and eukaryotic recombinant ASFV pB602L protein in vitro. The 474SKENLTPDE482 epitope in the ASFV pB602L C-terminus was identified as the minimal linear epitope for mAb 7E7 binding, with dozens of truncated pB602l fragments characterized by western blot assay. We also showed that this antigenic epitope sequence has a high conservation and antigenic index. Our study contributes to improved vaccine and antiviral development and provides new insights into the serologic diagnosis of ASF. KEY POINTS: • We developed a monoclonal antibody against ASFV pB602L, which can specifically recognize the ASFV Pig/HLJ/2018/ strain. • This study found one novel conserved B-cell epitope 474SKENLTPDE482. • In the 3D structure, 474SKENLTPDE482 is exposed on the surface of ASFV pB602L, forming a curved linear structure.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    为了诊断莱姆病,建议使用酶联免疫吸附试验检查莱姆病特异性血清抗体,所有酶联免疫吸附试验(ELISA)阳性或不明确的试验均通过免疫印迹确认.这种测量人体体液免疫的方法(例如,通过ELISA)数十年来提供了可靠且可重复的结果,并且类似的测定法已被验证用于监测B细胞免疫。这些检测伯氏疏螺旋体抗体的免疫学测试可用于常规诊断伯氏螺旋体病。不同的疏螺旋体物种的多样性及其不同的地理分布是世界上所有地理区域的标准和建议不相同的主要原因。与体液免疫相反,对疏螺旋体感染的T细胞反应或细胞免疫尚未得到很好的阐明,但是随着时间的推移,随着更多的研究,已经开发出一种新型的基于T细胞的检测方法(EliSpot),并验证了其对B.burgdorferi抗原特异性T细胞反应的灵敏检测。EliSpot莱姆试验可用于研究疏螺旋体感染引起的T细胞反应,它弥合了莱姆病中检测体液免疫和细胞免疫的能力之间的差距。此外,检测细胞免疫可能是莱姆病的实验室诊断测试,尤其是血清阴性莱姆病人.由于疏螺旋体感染的血清诊断方法经常提供假阳性和阴性结果,这种基于T细胞的诊断测试(细胞检测)可能有助于确认莱姆病的诊断.许多临床实验室确信,就检测潜在的疏螺旋体感染的灵敏度而言,细胞测定优于WesternBlot测定。研究还表明,在疏螺旋体感染中,体液和T细胞介导的细胞免疫反应之间存在分离。最后,数据表明,当血清学诊断未能做到这一点时,EliSpot莱姆试验可能有助于鉴定疏螺旋体感染个体.在本章中,体液和细胞免疫的配对用于评估患者的适应性反应。
    To diagnose Lyme Borreliosis, it is advised to use an enzyme-linked immunosorbent test to check for serum antibodies specific for Lyme and all tests with positive or ambiguous enzyme-linked immunosorbent assay (ELISA) results being confirmed by immunoblot. This method of measuring the humoral immunity in human fluids (e.g., by ELISA) has provided robust and reproducible results for decades and similar assays have been validated for monitoring of B cell immunity. These immunological tests that detect antibodies to Borrelia burgdorferi are useful in the diagnosis of Borreliosis on a routine basis. The variety of different Borrelia species and their different geographic distributions are the main reasons why standards and recommendations are not identical across all geographic regions of the world. In contrast to humoral immunity, the T cell reaction or cellular immunity to the Borrelia infection has not been well elucidated, but over time with more studies a novel T cell-based assay (EliSpot) has been developed and validated for the sensitive detection of antigen-specific T cell responses to B. burgdorferi. The EliSpot Lyme assay can be used to study the T cell response elicited by Borrelia infections, which bridges the gap between the ability to detect humoral immunity and cellular immunity in Lyme disease. In addition, detecting cellular immunity may be a helpful laboratory diagnostic test for Lyme disease, especially for seronegative Lyme patients. Since serodiagnostic methods of the Borrelia infection frequently provide false positive and negative results, this T cell-based diagnostic test (cellular assay) may help in confirming a Lyme diagnosis. Many clinical laboratories are convinced that the cellular assay is superior to the Western Blot assay in terms of sensitivity for detecting the underlying Borrelia infection. Research also suggests that there is a dissociation between the magnitude of the humoral and the T cell-mediated cellular immune responses in the Borrelia infection. Lastly, the data implies that the EliSpot Lyme assay may be helpful to identify Borrelia infected individuals when the serology-based diagnostic fails to do so. Here in this chapter the pairing of humoral and cellular immunity is employed to evaluate the adaptive response in patients.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

    求助全文

  • 文章类型: Journal Article
    犬埃里希菌病是一种重要的蜱传疾病,由埃里希菌属中的细菌引起,E.ewingii和E.chaffeensis导致严重的狗病。这项研究确定了肯尼亚和坦桑尼亚犬埃里希菌病抗体及其相关因素的发生。这是一项回顾性研究,评估了2016年至2021年期间提交给肯尼亚病理学家LancetKenya进行IDEXXSNAP4Dx™Plus测试的来自肯尼亚和坦桑尼亚的400个样本的实验室记录。检索了提交给病理学家柳叶刀肯尼亚兽医实验室进行诊断测试的所有样本的记录,检查,并编译。在分析过程中考虑了描述性统计以及单变量和多变量逻辑回归。测试为犬埃里希菌病阳性的样品的总比例为23%(92/400)。来自肯尼亚的样本占样本的61%(245/400),阳性百分比为31%(29/245)。来自坦桑尼亚的样本占39%(155/400),阳性百分比为69%(63/155)。在最终模型中,与1月至6月提交的样本相比,7月至12月提交的样本测试呈阳性的几率为1.7倍.与来自肯尼亚的狗相比,来自坦桑尼亚的狗的血液样本在SNAP测试中呈阳性的几率是5.31倍。这项研究报告表明,来自坦桑尼亚和今年下半年收到的样本中的阳性百分比很高。
    Canine ehrlichiosis is an important tick-borne disease caused by bacteria in the Ehrlichia genus with species such as E. canis, E. ewingii and E. chaffeensis resulting in a severe dog illness. This study determined the occurrence of canine ehrlichiosis antibodies and its associated factors in Kenya and Tanzania. This was a retrospective study that evaluated laboratory records of 400 samples from Kenya and Tanzania submitted to Pathologists Lancet Kenya for the IDEXX SNAP 4Dx™ Plus test between the years 2016 and 2021. Records of all samples submitted to the Pathologists Lancet Kenya veterinary laboratory for the diagnostic tests were retrieved, examined, and compiled. Descriptive statistics and univariable and multivariable logistic regression were considered during analysis. The overall proportion of samples that tested positive for canine ehrlichiosis was 23% (92/400). Samples from Kenya accounted for 61% (245/400) of samples, and the percent positive was 31% (29/245). The samples from Tanzania accounted for 39% (155/400), and the percent positive was 69% (63/155). In the final model, the odds of a sample testing positive was 1.7 times for those submitted from July to December compared with those submitted from January to June. Blood samples of dogs from Tanzania had 5.31 times the odds of testing positive on the SNAP test when compared with those from Kenya. This study reports high percent positive in samples originating from Tanzania and those received during the year\'s second half.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    可靠且有效的血清学测试对于监测针对SARS-CoV-2及其关注变体(VOC)的中和抗体至关重要。这里,我们提出了一个活的SARS-CoV-2中和试验的综合研究-临床平台,利用高度减毒的SARS-CoV-2(Δ3678_WA1-尖峰)。该菌株在病毒转录调控序列中包含突变和在开放阅读框3、6、7和8中的缺失,从而允许在生物安全水平2(BSL-2)实验室中进行安全处理。建立在这个骨干上,我们通过掺入修饰的绿色荧光蛋白序列(mGFP)构建了遗传稳定的报告病毒(mGFPΔ3678_WA1-spike)。我们还通过用变体BA.5和XBB.1.5尖峰替换WA1尖峰来构建mGFPΔ3678_BA.5-尖峰和mGFPΔ3678_XBB.1.5-尖峰,分别。在优化的荧光还原中和测定中,所有三种病毒在感染的细胞中表现出稳健的荧光信号和中和滴度,其与常规噬斑减少测定高度相关。此外,我们发现,简化的机器人辅助实验室到诊所的COVID-19中和测试工作流程非常敏感,具体,可重复,和准确的特征,允许在接受样品后24小时内评估针对SARS-CoV-2变体的中和滴度。总的来说,我们的创新方法为在BSL-2进行针对SARS-CoV-2和VOCs的临床样本的大规模检测提供了宝贵的途径,从而支持大流行准备和应对策略.
    A reliable and efficient serological test is crucial for monitoring neutralizing antibodies against SARS-CoV-2 and its variants of concern (VOCs). Here, we present an integrated research-clinical platform for a live SARS-CoV-2 neutralization assay, utilizing highly attenuated SARS-CoV-2 (Δ3678_WA1-spike). This strain contains mutations in viral transcription regulation sequences and deletion in the open-reading-frames 3, 6, 7, and 8, allowing for safe handling in biosafety level 2 (BSL-2) laboratories. Building on this backbone, we constructed a genetically stable reporter virus (mGFP Δ3678_WA1-spike) by incorporating a modified green fluorescent protein sequence (mGFP). We also constructed mGFP Δ3678_BA.5-spike and mGFP Δ3678_XBB.1.5-spike by substituting the WA1 spike with variants BA.5 and XBB.1.5 spike, respectively. All three viruses exhibit robust fluorescent signals in infected cells and neutralization titers in an optimized fluorescence reduction neutralization assay that highly correlates with a conventional plaque reduction assay. Furthermore, we established that a streamlined robot-aided Bench-to-Clinics COVID-19 Neutralization Test workflow demonstrated remarkably sensitive, specific, reproducible, and accurate characteristics, allowing the assessment of neutralization titers against SARS-CoV-2 variants within 24 h after sample receiving. Overall, our innovative approach provides a valuable avenue for large-scale testing of clinical samples against SARS-CoV-2 and VOCs at BSL-2, supporting pandemic preparedness and response strategies.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

公众号