进行本研究是为了评估饲喂常规玉米-大豆粉饮食的肉鸡硒(Se)作为硒酵母(SY)相对于亚硒酸钠(SS)的相对生物利用度。在完全随机的设计中,将总共360只一岁的ArborAcres商业肉鸡随机分配到5个处理中,每个处理6个重复,该设计涉及2(Se来源:SY和SS)×2(添加的Se水平:0.20和0.40mgSe/kg)处理的析因设计加1(未补充Se的对照饮食),持续42天。结果表明,血浆中硒的浓度,肝脏,心,乳房肌肉,第21天和第42天的肉鸡的胰腺和肾脏,第21天的胰腺以及第42天的胸肌和胰腺中的谷胱甘肽过氧化物酶(GSH-Px)活性,以及肝脏中的GSH-PxmRNA水平,心,第21天,随着添加硒水平的增加,胸肌和胰腺呈线性增加(p<.03)。此外,SY和SS之间的差异(p≤0.05)检测到血浆中的Se浓度,肝脏,心,乳房肌肉,胰腺和肾脏,第21天和第42天胰腺中的GSH-Px活性以及第21天胰腺中的GSH-PxmRNA水平。基于上述指数的多元线性回归的斜率比,当从血浆中的Se浓度计算时,SY相对于SS(100%)的Se生物利用度为111%-394%(p≤0.05),肝脏,心,乳房肌肉,胰腺,第21天和第42天胰腺中的肾脏和GSH-Px活性,以及第21天胰腺中的GSH-PxmRNA水平。这项研究的结果表明,与SS的Se相比,SY的Se更可用于提高血浆或组织中Se的浓度以及肉鸡胰腺中GSH-Px的表达和活性。
The present study was conducted to assess the relative bioavailability of selenium (Se) as Se yeast (SY) relative to sodium selenite (SS) for broilers fed a conventional corn-soybean meal diet. A total of 360 one-d-old Arbor Acres commercial broilers were randomly assigned to 5 treatments with 6 replicates per treatment in a completely randomized design involving a 2 (Se sources: SY and SS) × 2 (added Se levels: 0.20 and 0.40 mg Se/kg) factorial design of treatments plus 1 (a Se-unsupplemented control diet) for 42 days. The results showed that Se concentrations in plasma, liver, heart, breast muscle, pancreas and kidney of broilers on d 21 and 42, glutathione peroxidase (GSH-Px) activity in the pancreas on d 21 as well as in the breast muscle and pancreas on d 42, and GSH-Px mRNA levels in the liver, heart, breast muscle and pancreas on d 21 increased linearly (p < .03) as levels of added Se increased. Furthermore, a difference (p ≤ .05) between SY and SS was detected for Se concentrations in plasma, liver, heart, breast muscle, pancreas and kidney, GSH-Px activity in pancreas on both d 21 and 42, as well as pancreatic GSH-Px mRNA level on d 21. Based on slope ratios from the multiple linear regressions of the above indices, the Se bioavailabilities of SY relative to SS (100%) were 111%-394% (p ≤ .05) when calculated from the Se concentrations in plasma, liver, heart, breast muscle, pancreas, kidney and GSH-Px activities in pancreas on d 21 and 42, as well as GSH-Px mRNA level in pancreas on d 21. The results from this study indicated that the Se from SY was more available for enhancing the Se concentrations in plasma or tissues and the expression and activity of GSH-Px in pancreas of broilers than the Se from SS.