PDF(Pubmed)
Abstract：
Selenium is an essential trace element in our diet, crucial for the composition of human selenoproteins, which include 25 genes such as glutathione peroxidases and thioredoxin reductases. The regulation of the selenoproteome primarily hinges on the bioavailability of selenium, either from dietary sources or cell culture media. This selenium-dependent control follows a specific hierarchy, with \"housekeeping\" selenoproteins maintaining constant expression while \"stress-regulated\" counterparts respond to selenium level fluctuations. This study investigates the variability in fetal bovine serum (FBS) selenium concentrations among commercial batches and its effects on the expression of specific stress-related cellular selenoproteins. Despite the limitations of our study, which exclusively used HEK293 cells and focused on a subset of selenoproteins, our findings highlight the substantial impact of serum selenium levels on selenoprotein expression, particularly for GPX1 and GPX4. The luciferase reporter assay emerged as a sensitive and precise method for evaluating selenium levels in cell culture environments. While not exhaustive, this analysis provides valuable insights into selenium-mediated selenoprotein regulation, emphasizing the importance of serum composition in cellular responses and offering guidance for researchers in the selenoprotein field.
摘要：
硒是我们饮食中必需的微量元素,对人类硒蛋白的组成至关重要，其中包括25个基因，如谷胱甘肽过氧化物酶和硫氧还蛋白还原酶。硒蛋白质组的调节主要取决于硒的生物利用度，来自饮食来源或细胞培养基。这种依赖硒的控制遵循特定的层次结构，“管家”硒蛋白保持恒定表达，而“压力调节”对应物对硒水平波动作出反应。这项研究调查了市售批次中胎牛血清（FBS）硒浓度的变异性及其对特定应激相关细胞硒蛋白表达的影响。尽管我们的研究有局限性,专门使用HEK293细胞，专注于硒蛋白的子集，我们的发现强调了血清硒水平对硒蛋白表达的实质性影响，特别是对于GPX1和GPX4。荧光素酶报告基因测定法是一种灵敏而精确的方法，用于评估细胞培养环境中的硒水平。虽然不是详尽的，这项分析提供了对硒介导的硒蛋白调节的有价值的见解，强调血清成分在细胞反应中的重要性，并为硒蛋白领域的研究人员提供指导。
