生理生化指标的变化对围产期母羊的繁殖性能和健康至关重要。这项研究调查了采食量的时间变化,营养素消化率,血清参数,在产仔前第21、14和7天(Q21,Q14和Q7)和产仔后第3、7和14天(H3,H7和H14)进行瘤胃发酵。结果表明,产仔前干物质摄入量(dryi)和葡萄糖(Glu)逐渐降低(p<0.05),产仔后增加(p<0.05)。干物质消化率(DMD),粗蛋白(CPD),和酸性洗涤剂纤维(ADFD)增加(p<0.05)前,然后在H3天下降(p<0.05),然后在H14天上升(p<0.05)。瘤胃pH值,NH3-N,甘油三酯(TG)在产卵前逐渐升高(p<0.05),在第7天高于产卵后(p<0.05)。乙酸盐的浓度,丁酸盐,Q7天的总挥发性脂肪酸(T-VFA)低于Q21和Q14天(p<0.05),然后在产卵后增加(p<0.05)。总胆固醇(TC),高密度脂蛋白胆固醇(HDL-C),围产期母羊低密度脂蛋白胆固醇(LDL-C)浓度逐渐降低(p<0.05)。在第Q21天的BHBA和NEFA浓度低于从第Q14天至H14天的BHBA和NEFA浓度(p<0.05)。围产期母羊瘤胃微生物组成不同(p<0.05),和g_厌氧菌,g_肺细菌,G_Schwartzia与MI呈正相关(p<0.05),Glu,醋酸盐,丙酸盐,T-VFA,与LDL-C呈负相关(p<0.05)。g_Bacillus与dmi呈负相关(p<0.05),Glu,醋酸盐,丙酸盐,丁酸盐,T-VFA,但与瘤胃pH和LDL-C呈正相关(p<0.05)。总之,dmi,营养素消化率,瘤胃发酵,和母羊围产期血清参数的变化,和改变了的改变,血清葡萄糖,醋酸盐,丙酸盐,T-VFA与瘤胃细菌有关。
Changes in physiological and biochemical parameters are crucial for the reproductive performance and health of perinatal ewes. This study investigated the temporal variations in feed intake, nutrient digestibility, serum parameters, and ruminal fermentation on days 21, 14, and 7 before lambing (Q21, Q14, and Q7) and days 3, 7, and 14 after lambing (H3, H7, and H14). The results showed that dry matter intake (DMI) and glucose (Glu) gradually decreased (p < 0.05) before lambing and increased (p < 0.05) after lambing. The digestibility of dry matter (DMD), crude protein (CPD), and acid detergent fiber (ADFD) increased (p < 0.05) before lambing, then decreased (p < 0.05) on day H3, and then increased (p < 0.05) on day H14. The rumen pH, NH3-N, and triglycerides (TG) gradually increased (p < 0.05) before lambing and were higher (p < 0.05) on day Q7 than after lambing. The concentrations of acetate, butyrate, and total volatile fatty acids (T-VFA) were lower (p < 0.05) on day Q7 than those on days Q21 and Q14, then increased (p < 0.05) after lambing. Total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) concentrations gradually decreased (p < 0.05) in perinatal ewes. BHBA and NEFA concentrations were lower (p < 0.05) on day Q21 than those from days Q14 to H14. The rumen microbiota compositions were different (p < 0.05) in perinatal ewes, and g_Anaerovibrio, g_Lachnobacterium, and g_Schwartzia were positively correlated (p < 0.05) with DMI, Glu, acetate, propionate, and T-VFA, and negatively correlated (p < 0.05) with LDL-C. g_Bacillus was negatively correlated (p < 0.05) with DMI, Glu, acetate, propionate, butyrate, and T-VFA, but positively correlated (p < 0.05) with rumen pH and LDL-C. In summary, the DMI, nutrient digestibility, rumen fermentation, and serum parameters changed during the perinatal period of ewes, and the changes in DMI, serum glucose, acetate, propionate, and T-VFA were related to the rumen bacteria.