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Abstract：
Methane emissions from ruminants significantly contribute to greenhouse gases. This study explores the methane mitigation effect and mechanism of S. mcclurei through in vitro rumen fermentation, aiming to establish its potential as a feed additive. We investigated the effects of freeze-dried and dried S. mcclurei at supplementation levels of 2%, 5%, and 10% of dry matter on nutrient degradation, ruminal fermentation, methane inhibition, and microbial community structure in in vitro rumen fermentation. The freeze-dried S. mcclurei at 2% supplementation significantly reduced CH4 emissions by 18.85% and enhanced crude protein degradability. However, total VFA and acetate concentrations were lower in both treatments compared to the control. The microbial shifts included a decrease in Lachnospiraceae_NK3A20_group and Ruminococcus and an increase in Selenomonas, Succinivibrio, and Saccharofermentans, promoting propionate production. Additionally, a significant reduction in Methanomicrobium was observed, indicating direct methane mitigation. Freeze-dried S. mcclurei at a 2% supplementation level shows potential as an effective methane mitigation strategy with minimal impact on rumen fermentation, supported by detailed insights into microbial community changes.
摘要：
反刍动物的甲烷排放是温室气体的主要原因。本研究探讨了通过体外瘤胃发酵对S.mcclurei甲烷的缓解作用及其机制。旨在确立其作为饲料添加剂的潜力。我们调查了在2%的补充水平下冻干和干燥的S.mcclurei的效果，5%,和10%的干物质对养分降解，瘤胃发酵，甲烷抑制，瘤胃体外发酵过程中的微生物群落结构。添加2％的冻干S.mcclurei显着减少了18.85％的CH4排放，并提高了粗蛋白的降解性。然而,与对照相比,两种处理中的总VFA和乙酸浓度较低。微生物变化包括Lachnospispiraceae_NK3A20_群和Ruminococus的减少和硒单胞菌的增加，丁氏弧菌,和糖酵素，促进丙酸盐生产。此外,观察到甲烷微生物的显着减少，表明甲烷的直接缓解。2％补充水平的冻干S.mcclurei显示出潜在的有效甲烷缓解策略，对瘤胃发酵影响最小，由对微生物群落变化的详细见解支持。
