从根中分离出18株降解农药的内生细菌,茎,和健康水稻植物的叶片,并通过16SrRNA基因测序鉴定。此外,生化特性,包括酶的生产,染料降解,抗菌活性,促进植物生长的性状,包括N-固定,P-增溶,生长素生产,这些天然存在的内生细菌及其四个聚生体的ACC脱氨酶活性,被表征。阴沟肠杆菌HSTU-ABk39和肠杆菌。HSTU-ABk36显示抑制带41.5±1.5mm,对耐多药的人类病原菌金黄色葡萄球菌和表皮葡萄球菌29±09mm,分别。FT-IR分析表明,所有18个分离株均能够降解毒死蜱农药。我们的研究证实,来自水稻植物的农药降解内生细菌在促进植物生长中起关键作用。值得注意的是,在与内生细菌聚生体1,聚生体2,聚生体3或聚生体4混合的尿素减少(30%)的盆中生长的水稻植物表现出17.3%的增加,38.6%,18.2%,和39.1%的收益率,分别,与在含有100%肥料的盆中生长的对照植物相比。GC-MS/MS分析证实,聚生体处理导致毒死蜱降解为不同的无毒代谢产物,包括2-羟基-3,5,6三氯吡啶,甲烷膦酸二乙酯,磷硫酸盐亚砜,和Carbonochloridic。因此,这些分离物可以用作生物刺激剂,通过建立可持续的生物系统来提高作物产量。
Eighteen pesticide-degrading endophytic bacteria were isolated from the roots, stems, and leaves of healthy rice plants and identified through 16S rRNA gene sequencing. Furthermore, biochemical properties, including enzyme production, dye degradation, anti-bacterial activities, plant-growth-promoting traits, including N-fixation, P-solubilization, auxin production, and ACC-deaminase activities of these naturally occurring endophytic bacteria along with their four consortia, were characterized. Enterobacter cloacae HSTU-ABk39 and Enterobacter sp. HSTU-ABk36 displayed inhibition zones of 41.5 ± 1.5 mm, and 29 ± 09 mm against multidrug-resistant human pathogenic bacteria Staphylococcus aureus and Staphylococcus epidermidis, respectively. FT-IR analysis revealed that all eighteen isolates were able to degrade chlorpyrifos pesticide. Our study confirms that pesticide-degrading endophytic bacteria from rice plants play a key role in enhancing plant growth. Notably, rice plants grown in pots containing reduced urea (30%) mixed with either endophytic bacterial consortium-1, consortium-2, consortium-3, or consortia-4 demonstrated an increase of 17.3%, 38.6%, 18.2%, and 39.1% yields, respectively, compared to the control plants grown in pots containing 100% fertilizer. GC-MS/MS analysis confirmed that consortia treatment caused the degradation of chlorpyrifos into different non-toxic metabolites, including 2-Hydroxy-3,5,6 trichloropyridine, Diethyl methane phosphonate, Phorate sulfoxide, and Carbonochloridic. Thus, these isolates could be deployed as bio-stimulants to improve crop production by creating a sustainable biological system.