目的:用新型手持式瞳孔计评估健康受试者的瞳孔反应。
方法:对32名健康受试者(平均年龄21.2岁)的64只眼进行了测试。暗适应10分钟后,测量瞳孔对100cd/m2下1s红光和蓝光刺激的反应,从右眼到左眼,间隔1分钟。初始瞳孔大小(D1,mm),最小瞳孔大小(D2,mm),和收缩率(CR,%)获得。使用变异系数(CV,%)和Bland-Altman情节。使用类别间相关系数(ICC)和与常规设备的协议检查了检查者之间的一致性,按皮尔逊相关系数(r)计算。
结果:所有参数的CV在红色(11.0-20.7%)和蓝色(5.5-12.1%)光刺激中都具有很高的再现性。Bland-Altman图分析显示两种光刺激均无偏差。在红色(ICC=0.78-0.94)和蓝色(ICC=0.71-0.89)的光刺激中,考官之间获得了“几乎完美”和“实质性”的相关性。获得了设备之间的“优秀”和“良好”相关性,除了红色(D1:r=0.90;p<0.001,D2:0.72;p<0.001,和CR:0.08;p=0.631,分别)和蓝色(D1:r=0.87;p<0.001,D2:0.70;p<0.001,和CR:0.19;p=0.274,分别)光刺激的CR参数。
结论:新型瞳孔测量仪可用于评估瞳孔反应。然而,因为它们的结构不同,不能在设备之间直接比较CR值。
OBJECTIVE: To assess the pupil response with a new handheld pupillometer in healthy subjects.
METHODS: Sixty-four eyes of 32 healthy subjects (mean age 21.2 years) were tested. After dark adaptation for 10 min, pupil responses to 1 s red and blue light stimuli at 100 cd/m2 were measured in the order from right to left eyes with a 1 min interval. The initial pupil size (D1, mm), minimum pupil size (D2, mm), and constriction rate (CR, %) were obtained. Intra-examiner reproducibility was examined using the coefficient of variation (CV, %) and the Bland-Altman plot. Inter-examiner consistency was examined using the interclass correlation coefficient (ICC) and the agreements with a conventional device, by Pearson\'s correlation coefficient (r).
RESULTS: The CV of all parameters have high reproducibility in the red (11.0-20.7%) and blue (5.5-12.1%) light stimuli. Bland-Altman plot analysis showed no bias with both light stimuli. \"Almost perfect\" and \"substantial\" correlations between the examiners were obtained in the red (ICC = 0.78-0.94) and blue (ICC = 0.71-0.89) light stimuli. \"Excellent\" and \"good\" correlations between the devices were obtained, except for the CR parameter in the red (D1: r = 0.90; p < 0.001, D2: 0.72; p < 0.001, and CR: 0.08; p = 0.631, respectively) and blue (D1: r = 0.87; p < 0.001, D2: 0.70; p < 0.001, and CR: 0.19; p = 0.274, respectively) light stimuli.
CONCLUSIONS: The novel pupillometer is useful for assessing pupil response. However, because of their different constructions, the CR values cannot be compared directly between the devices.