■评估氧化锌与ajwain油(ZNOA)混合的新型组合以及ajwain和丁香酚(ZNOAE)的组合与常规使用的氧化锌丁香酚(ZNOE)对根管病原体如大肠杆菌和粪肠球菌的抗菌功效。
使大肠杆菌(MTCC443)和粪肠球菌(MTCC439)的纯培养物复活并在选择性培养基上生长。通过顺序稀释和琼脂孔扩散方法相应地确定了试验材料的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。根据临床和实验室标准研究所(CLSI)指南。数据值表示为平均值±标准偏差(SD)。组间比较通过单因素方差分析(ANOVA)检验,而群内配对比较使用非配对t检验完成(p<0.05)。
■ZNOE对大肠杆菌和粪肠杆菌的最小抑制浓度值分别为250和500µg/mL,ZNOA为250微克/毫升,和ZNOAE分别为125和250微克/毫升,相应地。ZNOE对大肠杆菌的抑制区形式的MBC值为21.33±1.53mm,ZNOA18.67±1.53mm,和ZNOAE20.33±1.53毫米。ZNOE的粪肠球菌抑制区为14.33±2.08mm,ZNOA18.67±2.08mm,和ZNOAE24.33±1.53毫米。
■所有测试材料均表现出良好的抗菌功效。然而,在测试材料的新颖组合之间,与ZNOE相比,ZNOA对耐药牙髓病原体的抗菌效果更好。
■达哈克PT,乔希党卫军,羽衣甘蓝YJ,etal.氧化锌与两种精油的新型组合在体外对牙髓病原体具有抗菌作用。国际临床研究中心2024;17(S-1):S11-S16。
UNASSIGNED: Evaluating the antimicrobial efficacy of the novel combinations of zinc oxide mixed with ajwain oil (ZNOA) and combination of ajwain and eugenol (ZNOAE) vs conventionally used zinc oxide eugenol (ZNOE) against endodontic pathogens like Escherichia coli and Enterococcus faecalis.
UNASSIGNED: The pure cultures of E. coli (MTCC 443) and E. faecalis (MTCC 439) were revived and grown on selective cultural media. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the test materials were determined correspondingly through sequential dilution and agar well diffusion methods, as per Clinical and Laboratory Standard Institute (CLSI) guidelines. The data values were presented as mean ± standard deviation (SD). The comparisons among groups were completed through the one-way analysis of variance (ANOVA) test, whereas intragroup pairwise comparisons were completed using the unpaired t-test (p < 0.05).
UNASSIGNED: Minimum inhibitory concentration values against E. coli and E. faecalis of ZNOE were 250 and 500 µg/mL, ZNOA was 250 µg/mL, and ZNOAE were 125 and 250 µg/mL, correspondingly. MBC values in the form of inhibition zone against E. coli by ZNOE were 21.33 ± 1.53 mm, ZNOA 18.67 ± 1.53 mm, and ZNOAE 20.33 ± 1.53 mm. The E. faecalis inhibition zone for ZNOE was 14.33 ± 2.08 mm, ZNOA 18.67 ± 2.08 mm, and ZNOAE 24.33 ± 1.53 mm.
UNASSIGNED: All test materials demonstrated good antibacterial efficacy. However, between the novel combinations of test materials, ZNOA showed better antimicrobial efficacy against resistant endodontic pathogens than ZNOE.
UNASSIGNED: Dahake PT, Joshi SS, Kale YJ, et al. A Novel Combination of Zinc Oxide with Two Essential Oils Exerts Antimicrobial Effect against Endodontic Pathogens In Vitro. Int J Clin Pediatr Dent 2024;17(S-1):S11-S16.