PDF(Pubmed)
Abstract：
Objectives: This preliminary animal study was conducted to assess the effects of chitosan as a novel obturation material for pulpectomized teeth on periapical inflammation, periodontal ligament (PDL) widening, and hard tissue resorption. Materials and Methods: Forty premolar root canals in two mature dogs were obturated with zinc oxide eugenol (ZOE) and an experimental 3% chitosan paste (n=20 in each group). The teeth were then restored with amalgam. After 28 days, the dogs were sacrificed, and histopathological assessment was performed. The amount of resorbed obturation material, degree of inflammatory response, degree of PDL widening, and the number of bone/cementum/dentin resorption defects were recorded under ×40 and ×200 magnifications. Data were analyzed using the Mann-Whitney U test, one-sample Wilcoxon signed-rank test, and Fisher\'s exact test (α=0.05). Results: Bone, cementum, and dentin resorption were seen in 6, 10, and 1 chitosan-obturated canals and 14, 15, and 0 ZOE-obturated canals, respectively. Only the bone resorption defects were significantly fewer in the chitosan group (P=0.026). Mild, moderate, and severe inflammation were observed in 17, 3, and 0 chitosan-obturated canals, and 7, 9, and 4 ZOE-filled canals, respectively (P=0.004). Mild, moderate, and severe PDL widening were seen around 15, 5, and 0 chitosan-filled canals and 7, 12, and 1 ZOE-filled canals, respectively (P=0.025). Conclusion: The 3% chitosan was superior to ZOE in terms of causing less inflammation and PDL widening. It also decreased bone resorption and acted similar to ZOE in terms of dentin and cementum resorption.
摘要：
目的：进行这项初步的动物研究，以评估壳聚糖作为一种新型的闭孔材料对牙髓切除牙齿的影响，牙周膜(PDL)增宽,和硬组织吸收。材料和方法：用氧化锌丁香酚（ZOE）和实验性3％壳聚糖糊（每组n=20）封闭两只成熟狗的40个前磨牙根管。然后用汞合金修复牙齿。28天后,狗被牺牲了，并进行组织病理学评估。再吸收的闭塞材料的量，炎症反应的程度，PDL加宽的程度，在×40和×200放大倍数下记录骨/牙骨质/牙本质吸收缺损的数量。数据使用Mann-WhitneyU检验进行分析，单样本Wilcoxon符号秩检验，和Fisher精确检验(α=0.05)。结果：骨，牙骨质,在6、10和1个壳聚糖闭塞的管道以及14、15和0个ZOE闭塞的管道中观察到牙本质吸收，分别。只有壳聚糖组的骨吸收缺损明显较少(P=0.026)。温和,中度,在17、3和0壳聚糖闭塞的管道中观察到严重的炎症，和7、9和4条充满ZOE的运河，分别为(P=0.004)。温和,中度,在15、5和0壳聚糖填充的运河以及7、12和1个ZOE填充的运河周围看到了严重的PDL加宽，分别为(P=0.025)。结论：3％壳聚糖在引起较少的炎症和PDL加宽方面优于ZOE。它还减少了骨吸收，在牙本质和牙骨质吸收方面与ZOE相似。
