滥用药物在世界范围内的广泛消费引起了人们的关注:它对公共卫生产生了不利影响,个人安全,和社会结构。专家们尤其感到震惊,因为在生物样本中越来越多地检测到新的精神活性物质。近年来,一些研究集中在开发方法来识别替代生物基质中的精神活性物质,比如汗水。这种方法有望监测物质的使用,尤其是在接受康复的个体中。在常用的分析程序中,使用一次性DPX吸头提取是一种新颖的,小型化,和有前途的技术。本研究旨在验证和应用一种方法来分析各种物质,包括安非他明,甲基苯丙胺,MDMA,MDA,MDEA,可卡因,cocathelee,脱水优宁甲酯,二丁酮,N-乙基戊酮,25E-NBOMe,25CNBOMe,2CC,2C-E,芬太尼,还有卡芬太尼,同时在汗液样本中。在这种方法中,通过使用实验室开发的贴片收集汗水,并用DPX-SCX尖端进行提取。气相色谱与质谱联用用于分离,为了识别,并量化分析物。验证结果表明,定量限范围为2至30ng分析物/片,并且该方法对于2至1100ng/patch的分析物浓度是线性的。将经过验证的方法应用于分析从志愿者吸毒者收集的30个汗液样本,并通过使用选定的离子模式(SIM)和全扫描进行处理。该方法能够检测和量化可卡因等物质,cocathelee,脱水优宁甲酯,MDMA,MDA,尼古丁,可替宁,咖啡因,普鲁卡因,利多卡因,和乙基安非他明同时。回收率为72.4%~97.1%。分析物在生物基质中是稳定的。总之,经过验证的方法被证明是有效的,并且允许在汗液样本中对目标分析物进行定量,强调汗水是分析滥用药物的可行矩阵。
Widespread consumption of drugs of abuse worldwide has caused concern: it adversely affects public health, individual safety, and social structures. Experts are particularly alarmed because new psychoactive substances have been increasingly detected in biological samples. In recent years, several studies have focused on developing methods to identify psychoactive substances in alternative biological matrices, such as sweat. This approach holds promise for monitoring substance use, especially in individuals undergoing rehabilitation. Among the commonly employed analytical procedures, extraction using disposable DPX tips stands out as a novel, miniaturized, and promising technique. This study aimed to validate and to apply a method to analyze various substances, including amphetamine, methamphetamine, MDMA, MDA, MDEA, cocaine, cocaethylene, anhydroecgonine methyl ester, dibutylone, N-ethylpentylone, 25E-NBOMe, 25CNBOMe, 2CC, 2C-E, fentanyl, and carfentanil, in sweat samples simultaneously. In this method, sweat is collected by using laboratory-developed patches, and extraction is conducted with DPX-SCX tips. Gas chromatography coupled to mass spectrometry is employed to separate, to identify, and to quantify the analytes. Validation results indicated that the quantification limit ranged from 2 to 30 ng of analyte/patch, and that the method was linear for analyte concentrations ranging from 2 to 1100 ng/patch. The validated method was applied to analyze 30 sweat samples collected from volunteers drug users and processed by using both the selected ion mode (SIM) and full scan. The method was able to detect and to quantify substances such as cocaine, cocaethylene, anhydroecgonine methyl ester, MDMA, MDA, nicotine, cotinine, caffeine, procaine, lidocaine, and ethylamphetamine simultaneously. The recovery rates ranged from 72.4 % to 97.1 %. The analytes were stable in the biological matrix. In conclusion, the validated method proved effective and allowed the target analytes to be quantified in sweat samples, highlighting that sweat is a viable matrix for analyzing drugs of abuse.