研究半月板细胞外基质降解。研究了马半月板(17匹马的n=34)。从三个区域(ROI;n=102)切割并评分位点匹配的切片,并进行组织学染色。蛋白聚糖(藏红蛋白O和快速绿色),aggrecan,和胶原蛋白裂解(NITEGE,DIPEN,和C1,2C抗体,分别)。进行了picrosirius红和二次谐波发生显微镜检查以研究胶原蛋白的超微结构。共有42个ROI符合纳入标准,并纳入最终分析。中位(范围)ROI组织学评分为3(0-9),提供了大量的病理学。中位数(范围)蛋白聚糖评分为1(0-3),代表浅表和中央半月板损失。DIPEN的中位数(范围),NITEGE,C1,2C评分为1(0-3),揭示股骨和胫骨表面的免疫染色。蛋白聚糖评分与组织学评估(p=0.03)和DIPEN评分(p=0.02)均呈显着正相关。此外,在两个聚集蛋白聚糖溶解指标之间观察到稳健的正相关(p=0.007),NITEGE和DIPEN分数。在NITEGE和组织学评分之间鉴定出负相关性(p=0.008)。C1,2C得分与任何其他得分无关。picrosiriusred和二次谐波发生显微镜(SHGM)显示了胶原蛋白基质和结构的中央损失。蛋白聚糖和胶原降解通常发生在半月板的表面和较不频繁的中心。中央半月板蛋白聚糖和胶原降解的鉴定提供了对中央半月板变性的新见解。然而,需要进一步的研究来阐明降解事件的病因和顺序.
Investigate meniscal extracellular matrix degradation. Equine menisci (n = 34 from 17 horses) were studied. Site-matched sections were cut and scored from three regions (ROIs; n = 102) and stained for histology,
proteoglycan (safranin O and fast green), aggrecan, and collagen cleavage (NITEGE, DIPEN, and C1,2C antibodies, respectively). Picrosirius red and second harmonic generation microscopy were performed to investigate collagen ultrastructure. A total of 42 ROIs met the inclusion criteria and were included in the final analysis. The median (range) ROI histological score was 3 (0-9), providing a large spectrum of pathology. The median (range)
proteoglycan score was 1 (0-3), representing superficial and central meniscal loss. The median (range) of DIPEN, NITEGE, and C1,2C scores was 1 (0-3), revealing immunostaining of the femoral and tibial surfaces. The
proteoglycan scores exhibited significant positive associations with both histologic evaluation (p = 0.03) and DIPEN scores (p = 0.02). Additionally, a robust positive association (p = 0.007) was observed between the two aggrecanolysis indicators, NITEGE and DIPEN scores. A negative association (p = 0.008) was identified between NITEGE and histological scores. The C1,2C scores were not associated with any other scores. Picrosirius red and second harmonic generation microscopy (SHGM) illustrated the loss of the collagen matrix and structure centrally.
Proteoglycan and collagen degradation commonly occur superficially in menisci and less frequently centrally. The identification of central meniscal
proteoglycan and collagen degradation provides novel insight into central meniscal degeneration. However, further research is needed to elucidate the etiology and sequence of degradative events.