■这项研究调查了储存在仓库中的豆粕(SBM)的蛋白质氧化以及SBM对生长性能的影响,抗氧化状态,消化性能,肠道形态学,和肉鸡的胸肌质量。
■总共,将160只1日龄的ArborAcresPlus肉鸡(一半雄性和一半雌性)随机分为两组,每组10只,每组8只:对照组的基础饮食包括储存在-20°C的SBM(FSBM),和实验组的基础饮食包括SBM储存在仓库在室温下45天(RSBM)。
■与FSBM相比,RSBM中的蛋白质羰基水平升高,游离和总硫醇水平和蛋白质的体外消化率降低。与FSBM相比,RSBM在第21天和第42天降低了血清谷胱甘肽(GSH)水平和肝脏总超氧化物歧化酶(T-SOD)活性。Further,RSBM降低十二指肠T-SOD活性,空肠过氧化氢酶(CAT),和T-SOD活性在第21天,并降低十二指肠CAT和T-SOD活性,空肠T-SOD活性,与FSBM相比,第21天和第42天的回肠GSH水平和T-SOD活性。此外,当饲喂含RSBM的饮食时,在第21天和第42天,肉鸡小肠中的胰蛋白酶活性和绒毛高度(VH)与隐窝深度(CD)之比降低。与FSBM相比,24小时滴水损失,剪切力,RSBM组24h和48h胸肌烹饪损失增加,对于48小时的肌肉亮度观察到相反的结果。
■室温储存45天导致蛋白质氧化并降低SBM的体外消化率,美联储RSBM损害了增长表现,抗氧化状态,和肉的质量,胰蛋白酶活性降低,并影响肉鸡小肠形态。
UNASSIGNED: This study investigated the protein oxidation of soybean meal (SBM) stored in a warehouse and the effects of SBM on growth performance, antioxidant status, digestive performance, intestinal morphology, and breast muscle quality of broilers.
UNASSIGNED: In total, 160 one-day-old Arbor Acres Plus broilers (half male and half female) were randomly divided into two groups with ten replicates of eight birds each: The control group was served with a basal diet including SBM stored at -20 °C (FSBM), and the experimental group was served with a basal diet including SBM stored in a warehouse at room temperature for 45 days (RSBM).
UNASSIGNED: Compared with FSBM, the protein carbonyl level in RSBM was increased, the free and total thiol levels and in vitro digestibility of protein were decreased. The RSBM decreased the serum glutathione (GSH) level and the hepatic total superoxide dismutase (T-SOD) activity at days 21 and 42 when compared with FSBM. Further, RSBM reduced the duodenal T-SOD activity, jejunal catalase (CAT), and T-SOD activities at day 21, and decreased the duodenal CAT and T-SOD activities, jejunal T-SOD activity, and ileal GSH level and T-SOD activity at days 21 and 42 when compared with FSBM. Besides, the trypsin activity and the ratio of villus height (VH) to crypt depth (CD) in small intestines of broilers at days 21 and 42 were reduced when fed with a RSBM-contained diet. Compared with FSBM, the 24-h drip loss, shear force, and 24- and 48-h cooking loss of breast muscle were increased of RSBM group, the opposite result was observed for muscle lightness at 48 h.
UNASSIGNED: Room temperature storage for 45 days led a protein oxidation and decreased in vitro digestibility in SBM, and fed RSBM impaired growth performance, antioxidant status, and meat quality, reduced trypsin activity, and affected the small intestine morphologyin broilers.