细胞外DNA,由受损的植物细胞释放,充当与损伤相关的分子模式(DAMP)。我们以前证明了小的棕色飞虱(Laodelphoxstriatellus,SBPH)以人工饮食为食时分泌的DNA酶II。然而,DNaseII在昆虫摄食中的功能仍然难以捉摸。通过抑制DNaseII的表达或应用异源表达的DNaseII研究了DNaseII对SBPH和水稻植物的影响。我们证明DNaseII主要在唾液腺中表达,并负责唾液的DNA降解活性。敲低DNaseII的表达导致水稻植株上SBPH的生长性能下降。dsDNaseII处理的SBPH不影响茉莉酸(JA),水杨酸(SA),乙烯(ET)途径,但引起了更高水平的H2O2和call体积累。在DNaseII缺陷的唾液中应用异源表达的DNaseII略微降低了伤口诱导的防御反应。我们提出了一种基于DNaseII的入侵模型,用于以寄主植物为食的SBPH,并为病虫害管理提供潜在目标。
Extracellular DNA, released by damaged plant cells, acts as a damage-associated molecular pattern (DAMP). We demonstrated previously that the small brown planthopper (Laodelphax striatellus, SBPH) secreted DNase II when feeding on artificial diets. However, the function of DNase II in insect feeding remained elusive. The influences of DNase II on SBPHs and rice plants were investigated by suppressing expression of DNase II or by application of heterogeneously expressed DNase II. We demonstrated that DNase II is mainly expressed in the salivary gland and is responsible for DNA-degrading activity of saliva. Knocking down the expression of DNase II resulted in decreased performance of SBPH reared on rice plants. The dsDNase II-treated SBPH did not influenced jasmonic acid (JA), salicylic acid (SA), ethylene (ET) pathways, but elicited a higher level of H2 O2 and callose accumulation. Application of heterogeneously expressed DNase II in DNase II-deficient saliva slightly reduced the wound-induced defence response. We propose a DNase II-based invading model for SBPH feeding on host plants, and provide a potential target for pest management.