感染性牛角膜结膜炎是全世界反刍动物中最关键的眼科疾病。莫拉氏菌是通常与这种疾病相关的细菌,并导致角膜炎,结膜炎,角膜溃疡,或失明。富血小板血浆(PRP)在动物和人类角膜溃疡和不同眼部浅表疾病中的作用是有益的,并促进快速愈合和改善,但是反刍动物对感染性角膜结膜炎的影响尚不确定。本研究旨在检查PRP对上皮再形成的影响,角膜组织,临床体征,和基质金属蛋白酶(MMP)在感染性角膜结膜炎绵羊中的表达。
■将18只羊分成三组并进行疾病诱导实验。第1组(G1)结膜下给药1.0mLPRP,第2组(G2)结膜下给药1.0mLPRP和50μL庆大霉素滴剂,对照组(CG)每12小时局部给予50μL生理盐水。荧光素染色,并进行了摄影。使用J-Image软件测量溃疡面积。手术后五天和十一天,每组一半的动物被安乐死,并通过组织病理学和酶谱对其角膜进行了评估。
■对照组和G2上皮化更快。CG表现出较少的眼部疾病临床体征。在组织病理学分析中,在G2中,仅在上皮中观察到改变。CG和G1在上皮中表现出改变,基质,和Descemet的膜。在酶谱中,在用PRP处理的动物中检测到MMP-2表达下降.基质金属蛋白酶-9在PRP单药治疗的动物中显著表达,而PRP+庆大霉素和CG引起下降。
■单独的富血小板血浆对上皮再形成没有任何有益作用,临床症状的下降,组织改变,和金属蛋白酶的表达。富血小板血浆联合庆大霉素能够抑制MMPs,主要是MMP-9,但不显示在上皮再形成的积极作用,临床体征减少,或组织效应。这些结果与在未经治疗的动物中发现的结果相似,因此,在感染性角膜结膜炎患者中使用PRP对绵羊没有更大的益处.需要进一步的研究来验证PRP在自然疾病表现中的使用结果。
UNASSIGNED: Infectious bovine keratoconjunctivitis is the most crucial ophthalmic disease among ruminants worldwide. Moraxella is the bacteria generally associated with this disease and leads to keratitis, conjunctivitis, corneal ulcers, or blindness. Platelet-rich plasma (PRP) effects in corneal ulcers and different ocular superficial diseases in animals and humans are beneficial and enhance rapid healing and improvement, but the effects in infectious keratoconjunctivitis in ruminants are uncertain. This study aimed to examine the effect of PRP on re-epithelization, corneal tissue, clinical signs, and matrix metalloproteinase (MMP) expression in sheep with infectious keratoconjunctivitis.
UNASSIGNED: Eighteen sheep were divided into three groups and subjected to a disease-induction experiment. Group 1 (G1) was administered 1.0 mL PRP subconjunctivally, Group 2 (G2) was administered 1.0 mL PRP subconjunctivally and 50 μL gentamicin drops, and the control group (CG) was administered 50 μL saline solution topically every 12 h. Clinical ophthalmologic examination, fluorescein staining, and photography were carried out. Ulcerated areas were measured employing J-Image software. Five and eleven days following the procedure, half of the animals from each group were euthanized, and their corneas were evaluated by histopathology and zymography.
UNASSIGNED: Control Group and G2 epithelialized more rapidly. The CG exhibited fewer clinical signs of ocular disease. In histopathological analysis, in G2, alterations were observed only in the epithelium. The CG and G1 exhibited alterations in the epithelium, stroma, and Descemet\'s membrane. In zymography, a decline in MMP-2 expression in the animals treated with PRP was detected. Matrix metalloproteinase-9 was significantly expressed in the animals treated with PRP monotherapy, whereas PRP + gentamicin and CG caused a decrease.
UNASSIGNED: Platelet-rich plasma alone did not demonstrate any beneficial effect on re-epithelialization, a decline in clinical signs, tissue alterations, and expression of metalloproteinases. Platelet-rich plasma combined with gentamicin was capable of suppressing MMPs, primarily MMP-9, but do not display positive effects in re-epithelization, reduction of clinical signs, or tissue effects. These outcomes are similar to those discovered in untreated animals, so the use of PRP in patients with infectious keratoconjunctivitis does not offer greater benefits in sheep. Additional research is required to validate the results of PRP use in natural disease presentation.