关键词: IBK Moraxella oculi Tifton1 infectious bovine keratoconjunctivitis pinkeye

Mesh : Cattle Animals Moraxella / genetics RNA, Ribosomal, 16S / genetics Keratoconjunctivitis, Infectious Phylogeny Cattle Diseases DNA, Bacterial / genetics Bacterial Typing Techniques Base Composition Sequence Analysis, DNA Fatty Acids / chemistry Keratoconjunctivitis Moraxellaceae Infections / veterinary Nucleotides

来  源:   DOI:10.1099/ijsem.0.006281

Abstract:
A novel species of the genus Moraxella was isolated from an ocular swab from a cow with infectious bovine keratoconjunctivitis. 16S rRNA gene sequencing suggested this species was Moraxella bovis (99.59 % nucleotide identity). Average nucleotide identity was calculated using a draft whole genome sequence of this strain compared with type strains of closely related Moraxella species and results established that it represents a new species. The genome size was 2 006 474 nucleotides and the G+C content was 42.51 mol%. The species could not be identified by matrix assisted laser desorption/ionization-time of flight mass spectrometry using a commercial database, confirming the novelty of the strain. We propose the name Moraxella oculi sp. nov. for this new species. The type strain is Tifton1T and has been deposited into the American Type Culture Collection (TSD-373T) and the National Collection of Type Cultures (NCTC), UK Health Security Agency (NCTC 14942T).
摘要:
从患有传染性牛角膜结膜炎的母牛的眼拭子中分离出莫拉氏菌属的一种新物种。16SrRNA基因测序表明该物种是牛莫拉氏菌(99.59%核苷酸同一性)。使用该菌株的全基因组序列草案与密切相关的莫拉氏菌物种的类型菌株进行比较,计算平均核苷酸同一性,并确定其代表新物种。基因组大小为2006474个核苷酸,G+C含量为42.51mol%。使用商业数据库通过基质辅助激光解吸/电离飞行时间质谱无法识别该物种,证实了菌株的新颖性。我们建议将其命名为Moraxellaoculisp。11月。对于这个新物种。类型菌株是Tifton1T,已被保存到美国类型培养物保藏中心(TSD-373T)和国家类型培养物保藏中心(NCTC),英国卫生安全局(NCTC14942T)。
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