多环芳烃(PAHs)具有植物毒性,这可能会限制它们的植物修复。当植物修复PAHs的能力受到损害时,植物生长调节剂的应用可以促进植物的生长。本研究旨在确定最佳的植物生长调节剂(1-萘乙酸,6-苄基腺嘌呤,或噻二氮隆),以增强甜草(狼尾草cv。Mahasarakham)生长在受PAH污染的土壤中。在温室研究中,与其他测试的植物生长调节剂(干重24.11±1.28g和干重0.70±0.02g)相比,0.01mg/l噻地隆导致甜草的生长最高。甜草生长在被PAH污染的土壤中,通过减少总叶绿素(1.01µg/g鲜重)和类胡萝卜素(0.28µg/g鲜重)含量,脯氨酸增加(6.63µg/g鲜重),证明了对甜草的毒性。同时,总叶绿素,类胡萝卜素,在未污染土壤中生长的甜草叶片中的脯氨酸含量分别为1.68、0.44和5.23µg/g鲜重,分别。当甜草被用于植物修复PAHs时,乙炔减少(4.69±0.50%),萘(10.69±1.47%),和菲(3.61±0.07%),与非种植土壤中超过30%的水平相比。对于三种PAHs,生物富集因子为1.6至2.4,但易位因子低于1,显示出有限的移动到植物的地上部分,从而表明其主要机制是根治。甜草是PAH修复的绝佳候选者,特别是当噻二唑用于缓解植物胁迫时。
与1-萘乙酸和6-苄基腺嘌呤相比,噻二氮隆在0.01mg/l时是刺激甜草生长的最佳植物生长调节剂。甜草增强了对乙炔的去除,萘,和污染土壤中的菲。多环芳烃(PAH)修复的可能机制是根治修复,由于PAH在甜草生物量中的积累是有限的,从根到芽的易位因子低于1。噻二唑隆可能通过增加植物对PAH毒性的耐受性间接增强甜草对PAH的植物修复。
Polycyclic aromatic hydrocarbons (PAHs) are phytotoxic, which can limit their
phytoremediation. When the ability of plants to
phytoremediation PAHs is compromised, the application of plant growth regulators can enhance the growth of the plants. This study aimed to determine the best plant growth regulator (1-naphthalene acetic acid, 6-benzyladenine, or thidiazuron) to enhance the
phytoremediation ability of sweet grass (Pennisetum purpureum cv. Mahasarakham) when grown in weather PAH-contaminated soil. In a greenhouse study, 0.01 mg/l thidiazuron resulted in the highest growth of sweet grass when compared to the other tested plant growth regulators (dry shoot weight 24.11 ± 1.28 g and dry root weight 0.70 ± 0.02 g). Sweet grass was grown in soil contaminated with PAH, which demonstrated the toxicity to sweet grass by reducing the total chlorophyll (1.01 µg/g fresh weight) and carotenoid (0.28 µg/g fresh weight) contents with proline increased (6.63 µg/g fresh weight). Meanwhile, total chlorophyll, carotenoid, and proline content in leaves of sweet grass grown in non-contaminated soil were 1.68, 0.44, and 5.23 µg/g fresh weight, respectively. When sweet grass was used to phytoremediate PAHs, there were reductions in acenaphthylene (4.69 ± 0.50%), acenaphthene (10.69 ± 1.47%), and phenanthrene (3.61 ± 0.07%), which compared to levels of over 30% in non-planted soil. For the three PAHs, the bioconcentration factors were 1.6 to 2.4, but the translocation factors were below 1, showing limited movement to the aerial parts of the plant, thereby suggesting that the main mechanism is rhizoremediation. Sweet grass is an excellent candidate for PAH remediation, especially when thidiazuron is applied to relieve plant stress.
Thidiazuron at 0.01 mg/l was the best plant growth regulator to stimulate the growth of sweet grass when compared to 1-naphthalene acetic acid and 6-benzyladenine. Sweetgrass enhanced the removal of acenaphthylene, acenaphthene, and phenanthrene from contaminated soil. A possible mechanism for polycyclic aromatic hydrocarbon (PAH) remediation was rhizoremediation, as the accumulation of PAH in sweet grass biomass was limited, and the translocation factor from the root to the shoot was lower than 1. Thidiazuron may indirectly enhance the PAH
phytoremediation by sweet grass via increased plant tolerance to PAH toxicity.