牛皮癣,炎症性自身免疫性皮肤病,以鳞片状白色或红斑为特征,严重影响患者的生活质量和社会活动。源自人脐带的间充质干细胞(UCMSCs)代表了一种有前途的治疗牛皮癣的方法,因为它在伦理同意的独特优势,丰富的来源,高增殖能力,和免疫抑制。尽管冷冻保存为细胞治疗提供了多种益处,由于受损的细胞功能,它也极大地损害了MSC的临床益处。本研究旨在评估冷冻保存的UCMSCs在牛皮癣小鼠模型以及牛皮癣患者中的治疗效果。我们的结果表明,冷冻保存和新鲜的UCMSCs在抑制银屑病样症状如增厚方面具有相当的效果,红斑,和缩放,和血清IL-17A分泌在银屑病小鼠模型中的作用。此外,注射冷冻保存的UCMSCs的银屑病患者在银屑病面积和严重程度指数(PASI)方面有显著改善,医师全球评估(PGA),和与基线值相比的患者全球评估(PtGA)评分。机械上,冷冻保存的UCMSCs明显抑制PHA激活的PBMCs的增殖,1型T辅助(Th1)和17型T辅助(Th17)细胞分化和分泌炎症细胞因子,包括IFN-γ,抗CD3/CD28珠刺激的PBMC中的TNF-α和IL-17A。一起来看,这些数据表明,冷冻保存的UCMSCs对银屑病表现出巨大的有益作用.因此,冷冻保存的UCMSCs可以作为牛皮癣治疗的现成细胞产品全身给药。试用注册ChiCTR1800019509。2018年11月15日注册-追溯注册,http://www。chictr.org.cn/.
Psoriasis, an inflammatory autoimmune skin disease, is characterized by scaly white or erythematous plaques, which severely influence patients\' quality of life and social activities. Mesenchymal stem cells derived from the human umbilical cord (UCMSCs) represent a promising therapeutic approach for psoriasis because of its unique superiority in ethical agreeableness, abundant source, high proliferation capacity, and immunosuppression. Although cryopreservation provided multiple benefits to the cell therapy, it also greatly compromised clinical benefits of MSCs due to impaired cell functions. The current study aims to evaluate the therapeutic efficacy of cryopreserved UCMSCs in a mouse model of psoriasis as well as in patients with psoriasis. Our results showed that cryopreserved and fresh UCMSCs have comparable effects on the suppression of psoriasis-like symptoms such as thickening, erythema, and scaling, and serum IL-17 A secretion in mice model of psoriasis. Moreover, psoriatic patients injected with cryopreserved UCMSCs had a significant improvement in the Psoriasis Area and Severity Index (PASI), Physician Global Assessment (PGA), and Patient Global Assessments (PtGAs) scores compared to baseline values. Mechanically, cryopreserved UCMSCs markedly inhibit the proliferation of PHA-activated PBMCs, type 1 T helper (Th1) and type 17 T helper (Th17) cell differentiation and secretion of inflammatory cytokines including IFN-γ, TNF-a and IL-17 A in PBMCs stimulated by anti-CD3/CD28 beads. Taken together, these data indicated that cryopreserved UCMSCs exhibited great beneficial effect on psoriasis. Thus, cryopreserved UCMSCs can be systemically administered as \'\'off-the-shelf\'\' cell product for psoriasis therapy. Trial Registration ChiCTR1800019509. Registered on November 15, 2018-Retrospectively registered, http://www.chictr.org.cn/ .