■在这项研究中,我们提出了一种电化学传感器,用于检测氧维酮(Oxyp)和普兰辛金(Pra),两种天然呋喃香豆素衍生物。确定这些分子对DNA的影响对于作为潜在的候选药物是重要的。我们的研究重点是探索这些化合物的电化学行为及其与DNA的相互作用。
■通过评估Oxyp和Pra的氧化电流,系统地分析了它们的电化学性能。在溶液相和电极表面相互作用之前和之后,监测鸟嘌呤碱的氧化电流和峰值电位的变化。
■Oxyp的检测限(LOD)和定量限(LOQ)确定为1.3和4.3μg/mL,分别。对于Pra来说,LOD和LOQ分别为20和68μg/mL,分别。稳定性研究表明,Oxyp溶液保持其氧化能力超过一个月,而Pra溶液保持其氧化能力近120分钟。我们的发现表明Oxyp与dsDNA相互作用,可能通过静电相互作用,有望成为靶向DNA的潜在候选药物。另一方面,Pra与dsDNA的相互作用需要进一步探索以充分理解其作用方式。
■本研究开发的电化学传感器为检测和分析这些天然化合物与dsDNA的相互作用提供了一种可靠而有效的方法。我们的研究有助于促进对天然呋喃香豆素和dsDNA之间相互作用的理解,为设计和开发新型有效的DNA靶向药物奠定基础。
UNASSIGNED: In this study, we present an electrochemical sensor for the detection of oxypeucedanin (Oxyp) and prantschimgin (Pra), two natural furanocoumarin derivatives. The determination of the effects of these molecules on DNA is important to be potential drug candidates. Our research focused on exploring the electrochemical behaviour of these compounds and their interaction with DNA.
UNASSIGNED: The electrochemical properties of Oxyp and Pra were systematically analyzed by evaluating their oxidation currents. Changes in the oxidation currents and peak potentials of guanine bases were monitored before and after interaction in the solution phase and at the electrode surface.
UNASSIGNED: The limit of detection (LOD) and limit of quantitation (LOQ) for Oxyp were determined to be 1.3 and 4.3 μg/mL, respectively. For Pra, the LOD and LOQ were found to be 20 and 68 μg/mL, respectively. Stability studies demonstrated that the Oxyp solution retained its oxidation capacity for over a month, whereas the Pra solution retained its oxidation capacity for nearly 120 min. Our findings suggest that Oxyp interacts with dsDNA, potentially through electrostatic interactions, showing promise as a potential drug candidate targeting DNA. On the other hand, the interaction of Pra with dsDNA requires further exploration to fully understand its mode of action.
UNASSIGNED: The electrochemical sensor developed in this study provides a reliable and efficient method for detecting and analysing the interaction of these natural compounds with dsDNA. Our research contributes to advancing the understanding of the interaction between natural furanocoumarins and dsDNA, laying the groundwork for the design and development of novel and effective DNA-targeted drugs.