pathological findings

病理结果
  • 文章类型: Journal Article
    欧洲刺猬(Erinaceuseuropaeus)是在城市地区经常发现的夜间食虫动物。在过去的几十年里,他们的人口在不同的欧洲国家已经下降,人类活动已经成为这一趋势的重要贡献者。虽然文献主要集中在创伤作为死亡的主要原因,很少有作者考虑过病理结果。本研究基于对意大利的162只欧洲刺猬和瑞士的109只刺猬进行的全面验尸结果。与以往的研究不同,主要死亡原因是传染病(60.5%),其次是创伤性侮辱(27.7%)。肺部是受影响的主要器官,主要表现为淋巴浆细胞(45.9%),肉芽肿性(18.1%)或化脓性(8.2%)肺炎。在所有肺部的57.2%中检测到线虫,并且与肺炎显着相关(p值<0.001)。据我们所知,这是第一个报告传染病是刺猬死亡的主要原因的研究,强调野生动物救援中心需要采取适当的诊断和治疗措施。需要进一步的研究来确定影响该物种的广泛感染因子,并阐明它们与宿主的相互作用。最后,应实施公民敏化,以促进负责任的行为,以减少与人类有关的创伤事件。
    European hedgehogs (Erinaceus europaeus) are nocturnal insectivores frequently found in urban areas. In the last decades, their population has declined in various European countries and human activities have emerged as significant contributors to this trend. While the literature has mainly focused on trauma as the major cause of mortality, few authors have considered pathological findings. The present study is based on the results of full post-mortem examinations performed on 162 European hedgehogs in Italy and 109 in Switzerland. Unlike in previous studies, the main cause of mortality was infectious diseases (60.5%), followed by traumatic insults (27.7%). The lungs were the main organ affected, showing mostly lymphoplasmacytic (45.9%), granulomatous (18.1%) or suppurative (8.2%) pneumonia. Nematodes were detected in 57.2% of all lungs and were significantly associated with pneumonia (p-value < 0.001). To our knowledge, this is the first study to report infectious diseases as the main cause of hedgehog death, emphasizing the need for wildlife rescue centers to adopt appropriate diagnostic and therapeutic measures. Further research is necessary to determine the broad range of infectious agents that affect this species and elucidate their interplay with the host. Finally, citizen sensitization should be implemented to promote responsible behaviors that could reduce human-related traumatic events.
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  • 文章类型: Case Reports
    颅颈交界区(CCJ)的动静脉瘘(AVF)和硬膜内AVF通常与动脉瘤和静脉曲张有关,有时很难在放射学图像上识别破裂点。我们报告了一种情况,其中血管壁磁共振图像(VW-MRI)可用于识别CCJAVF的破裂点。
    一名70岁的男子突然出现头痛。他患有格拉斯哥昏迷量表E4V5M6,世界神经外科学会联合会(WFNS)I级。在头部计算机断层扫描中发现了Fisher组3蛛网膜下腔出血和脑积水。脑血管造影显示颈椎C1水平的脊髓AVF。磁共振图像增强的运动敏化驱动平衡(MSDE方法在部分AVF引流静脉中显示出增强作用,但是这个病变的血管结构是不确定的。我们对急性脑积水和降压治疗进行了持续脑室引流。发病后30天进行脑血管造影,并在AVF引流静脉的一部分中发现了动脉瘤结构,VW-MRI最初增强了。发病后第38天,他接受了直接手术以闭塞AV瘘并解剖动脉瘤结构。组织病理学显示动脉瘤结构为静脉曲张伴淋巴细胞浸润,在静脉曲张附近观察到含铁血黄素沉积。
    最近,据报道,VW-MRI显示硬脑膜AVF静脉曲张增强与破裂病例之间存在关联。VW-MRI,特别是增强的MSDE方法,可能有助于估计动静脉分流疾病的破裂点。
    UNASSIGNED: Arteriovenous fistulas (AVFs) of the craniocervical junction (CCJ) and intradural AVFs are often associated with aneurysms and varics, and it is sometimes difficult to identify the ruptured point on radiological images. We report a case in which vessel wall magnetic resonance image (VW-MRI) was useful for identifying the ruptured point at the CCJ AVF.
    UNASSIGNED: A 70-year-old man presented with a sudden onset of headache. He had Glasgow Coma Scale E4V5M6, world federation of neurosurgical societies (WFNS) Grade I. Fisher group 3 subarachnoid hemorrhage and hydrocephalus were found on head computed tomography. Cerebral angiography showed a spinal AVF at the C1 level of the cervical spine. Magnetic resonance image-enhanced motion sensitized driven equilibrium (MSDE-method showed an enhancing effect in part of the AVF draining vein, but the vascular architecture of this lesion was indeterminate. We performed continuous ventricular drainage for acute hydrocephalus and antihypertensive treatment. Cerebral angiography was performed 30days after the onset of the disease, and was revealed an aneurysmal structure in a portion of the AVF draining vein, which VW-MRI initially enhanced. On the 38th day after onset, he underwent direct surgery to occlude the AV fistula and dissect the aneurysmal structure. Histopathology showed that the aneurysmal structure was varices with lymphocytic infiltration, and hemosiderin deposition was observed near the varices.
    UNASSIGNED: Recently, VW-MRI has been reported to show an association between the enhancement of varices in dural AVF and rupture cases. VW-MRI, especially the enhanced MSDE method, may be useful in estimating the ruptured point in arteriovenous shunt disease.
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  • 文章类型: Journal Article
    胃肠道的内镜病理学发现对结直肠癌(CRC)的早期诊断至关重要。以前的深度学习作品,旨在提高CRC检测性能并减少主观分析错误,仅限于息肉分割。没有考虑病理学发现,只有卷积神经网络(CNN),无法处理全局图像特征信息,被利用。这项工作介绍了一种新颖的基于视觉变换器(ViT)的早期CRC检测方法。所提出的方法的核心组成部分是ViTcol,用于对内窥镜病理学发现进行分类的增强视觉转换器,和PUTS,基于视觉转换器的息肉分割模型。结果表明,这种基于视觉变压器的CRC检测方法优于现有的CNN和视觉变压器模型。ViTCol在对病理结果进行分类方面表现突出,在Kvasir数据集上,受试者工作曲线下面积(AUC)值为0.9999±0.001。PUTS在分割息肉图像方面提供了出色的结果,在Kvasir-SEG和CVC-Clinic数据集上,联合平均交集(mIoU)为0.8673和0.9092,分别。这项工作强调了空间变换器在定位输入图像中的价值,可以无缝集成到主视觉变压器网络中,增强了早期CRC检测的关键图像特征的自动识别。
    Endoscopic pathological findings of the gastrointestinal tract are crucial for the early diagnosis of colorectal cancer (CRC). Previous deep learning works, aimed at improving CRC detection performance and reducing subjective analysis errors, are limited to polyp segmentation. Pathological findings were not considered and only convolutional neural networks (CNNs), which are not able to handle global image feature information, were utilized. This work introduces a novel vision transformer (ViT)-based approach for early CRC detection. The core components of the proposed approach are ViTCol, a boosted vision transformer for classifying endoscopic pathological findings, and PUTS, a vision transformer-based model for polyp segmentation. Results demonstrate the superiority of this vision transformer-based CRC detection method over existing CNN and vision transformer models. ViTCol exhibited an outstanding performance in classifying pathological findings, with an area under the receiver operating curve (AUC) value of 0.9999 ± 0.001 on the Kvasir dataset. PUTS provided outstanding results in segmenting polyp images, with mean intersection over union (mIoU) of 0.8673 and 0.9092 on the Kvasir-SEG and CVC-Clinic datasets, respectively. This work underscores the value of spatial transformers in localizing input images, which can seamlessly integrate into the main vision transformer network, enhancing the automated identification of critical image features for early CRC detection.
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  • 文章类型: Case Reports
    毛细血管瘤是一种罕见的良性血管瘤,发生在皮肤软组织中,轨道,头部,和脖子。颅内病例,尤其是实质内的病例,极为罕见。在这项研究中,我们报告了颅内实质性毛细血管瘤的过程,伴有左上肢轻度运动性轻瘫和不自主运动,并通过手术切除成功治疗。包括放射学和病理学检查。
    这是一例60岁女性,表现为运动无力和左上肢不自主运动。计算机断层扫描和磁共振成像显示右额叶出血性肿块病变,但未增强造影剂。脑血管数字减影血管造影显示无血管染色及动静脉分流异常。术前,我们诊断为海绵状血管瘤,出血性成分位于右运动皮质。因为这个病例是有症状的,我们对右侧额叶病变进行了开颅手术和大体全切除.毛细血管瘤的诊断是通过组织学检查得出的。包括免疫组织学研究。
    因为脑实质内毛细血管瘤难以通过术前影像学诊断,手术治疗,组织病理学检查很重要。
    UNASSIGNED: Capillary hemangioma is a rare benign hemangioma that occurs in the soft tissues of the skin, orbit, head, and neck. Intracranial cases, especially intraparenchymal cases, are extremely rare. In this study, we report the course of an intracranial parenchymal capillary hemangioma with left mild motor paresis and involuntary movements of the left upper extremity and was successfully treated by surgical resection, including radiological and pathological examinations.
    UNASSIGNED: This is a case of a 60-year-old woman who presented with motor weakness and involuntary movement of the left upper extremity. Computed tomography and magnetic resonance imaging revealed the right frontal hemorrhagic mass lesion without enhancement of contrast medium. Cerebral digital subtraction angiography showed no vascular stain and abnormal arteriovenous shunt. Preoperatively, we diagnosed cavernous hemangioma with a hemorrhagic component located in the right motor cortex. Because this case was symptomatic, we performed a craniotomy and gross total resection of the right frontal lesion. The diagnosis of capillary hemangioma was made by histological examination, including immunohistological study.
    UNASSIGNED: Because intraparenchymal capillary hemangiomas are difficult to diagnose with preoperative imaging, surgical treatment, and histopathological examination are important.
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  • 文章类型: Journal Article
    目的:探讨术前超声造影(CEUS)对胰腺导管腺癌(PDAC)术后早期复发的预测价值。
    方法:2017年1月至2022年9月,共纳入110例术前1个月内接受CEUS检查的PDAC患者(均为手术标本证实)。我们提出了五种CEUS增强模式(模式I,均匀增强;模式II,无囊性成分的异质增强;模式III,环增强;模式四,繁星增强;模式V,具有囊性成分的异质增强)。分析了预测早期复发(根治性切除术后1年内复发)的临床病理和CEUS特征。将重要的CEUS特征与病理结果进行比较。
    结果:肿瘤大小和TNM分期与早期复发密切相关。不完全增强和增强模式III,在CEUS成像中IV和V更容易出现早期复发。不完全强化病变具有较高的组织学肿瘤等级,较少的剩余腺泡,肿瘤内更频繁的坏死。具有I型和II型的PDAC具有较低的组织学肿瘤等级,模式三,IV和V具有较高的组织学肿瘤分级。具有模式I的PDAC,II和IV的肿瘤内坏死频率低于III和V型的PDAC,与模式IV的PDAC具有较低的MVD值。
    结论:具有不完全增强和增强模式III的PDAC,IV和V在尝试根治性切除后更容易出现早期复发,这些重要的CEUS特征与PDAC的病理表现密切相关。
    OBJECTIVE: To identify characteristics of preoperative contrast-enhanced ultrasound (CEUS) that could predict early recurrence after curative resection of pancreatic ductal adenocarcinoma (PDAC).
    METHODS: From January 2017 to September 2022, a total of 110 patients with PDAC (all confirmed by samples obtained via operation) who underwent CEUS within 1 month before surgery were enrolled. We proposed five CEUS enhancement patterns (Pattern I, homogeneous enhancement; Pattern II, heterogeneous enhancement without cystic components; pattern III, ring enhancement; Pattern IV, starry enhancement; Pattern V, heterogeneous enhancement with cystic components) of PDAC. Clinical-pathologic and CEUS characteristics for predicting early recurrence (recurrence within 1 year after curative resection) were analyzed. Important CEUS characteristics were compared with the pathological findings.
    RESULTS: Tumor size and TNM stage were closely associated with early recurrence. Incomplete-enhancement and enhancement pattern III, IV and V at CEUS imaging were more prone to early recurrence. Incomplete-enhancement lesions had higher histological tumor grades, less frequent remaining acini, and more frequent necrosis within the tumor. PDACs with pattern I and II had lower histological tumor grades, and pattern III, IV and V had higher histological tumor grades. PDACs with pattern I, II and IV had less frequent intratumoral necrosis than PDACs with pattern III and V, and PDACs with pattern IV had lower MVD values.
    CONCLUSIONS: PDACs with incomplete enhancement and enhancement pattern III, IV and V were more prone to early recurrence after attempted curative resection, and these important CEUS characteristics were closely related to the pathological findings of PDAC.
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  • 文章类型: Journal Article
    肝脏炎性假瘤(IPT)被定义为良性,非恶性,以肌纤维母细胞梭形细胞为特征的非转移性肿瘤,炎症细胞的异源性群体,特别是浆细胞,淋巴细胞和巨噬细胞,以及纤维化和坏死的位置,没有细胞间变或非典型有丝分裂。尽管参考文献中有随后的报道,肝脏IPT仍然难以诊断;与其他各种良性疾病和恶性肝肿瘤相比,其鉴别诊断存在重大问题。组织病理学检查结果始终是确认诊断的必要条件,特别是考虑到IPT的发病机制至今仍不明确。肝脏IPT在其临床特征方面是一种异质实体,病理结果,和发病机制。一旦确诊,然而,应避免不必要的手术,如楔形切除术和肺叶切除术。这里,我们讨论了肝脏IPT的异质性,其临床特征,病理结果,和发病机制,并描述其鉴别诊断。
    Hepatic inflammatory pseudotumors (IPTs) are defined as benign, non-malignant, non-metastasizing tumors characterized by the presence of myofibroblastic spindle cells, hetorogenous populations of inflammatory cells, particularly plasma cells, lymphocytes and macrophages, as well as locations of fibrosis and necrosis without cellular anaplasia or atypical mitoses. Despite subsequent reports in the references, hepatic IPT remains difficult to diagnose; while posing major issues specifically for its differential diagnosis compared with that of other various benign diseases and malignant hepatic tumors. Histopathological findings are always a requisite for confirming the diagnosis, particularly given that the pathogenesis of IPT remains ambiguous to date. Hepatic IPT is a heterogeneous entity in terms of its clinical features, pathological findings, and pathogenesis. Once the diagnosis is confirmed, however, needless surgery such as wedge resection and lobectomy should be avoided. Here, we discuss the heterogeneity of hepatic IPT, its clinical features, pathological findings, and pathogenesis, and describe its differential diagnosis.
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  • 文章类型: Journal Article
    全身尸检可能对了解2019年冠状病毒病(COVID-19)病理生理学至关重要。我们旨在分析一系列全身尸检的病理结果,特别关注超级感染。
    这是一项前瞻性多中心研究,包括2020年4月至2021年2月进行的70例COVID-19尸检。流行病学,使用标准化病例报告表收集临床和病理信息.
    年龄中位数(IQR)为70岁(范围为63.75-74.25岁),76%的病例为男性。大多数患者(90%,)在COVID-19诊断之前至少有一种合并症,血管危险因素是最常见的。65.71%的患者在随访期间出现感染性并发症。大多数患者需要机械通气(75.71%),主要是有创的。在多变量分析中,住院时间和有创机械通气与感染显著相关(分别为p=0.036和p=0.013).尸检结果显示肺部弥漫性肺泡损伤,心脏左心室肥厚,肝脏脂肪变性和感染前的心脏和肾脏动脉硬化。
    我们的研究证实了大型患者系列中归因于COVID-19的主要尸检组织病理学发现,同时强调病理中合并症和超感染的重要性。
    UNASSIGNED: Whole-body autopsies may be crucial to understand coronavirus disease 2019 (COVID-19) pathophysiology. We aimed to analyze pathological findings in a large series of full-body autopsies, with a special focus on superinfections.
    UNASSIGNED: This was a prospective multicenter study that included 70 COVID-19 autopsies performed between April 2020 and February 2021. Epidemiological, clinical and pathological information was collected using a standardized case report form.
    UNASSIGNED: Median (IQR) age was 70 (range 63.75-74.25) years and 76% of cases were males. Most patients (90%,) had at least one comorbidity prior to COVID-19 diagnosis, with vascular risk factors being the most frequent. Infectious complications were developed by 65.71% of the patients during their follow-up. Mechanical ventilation was required in most patients (75.71%) and was mainly invasive. In multivariate analyses, length of hospital stay and invasive mechanical ventilation were significantly associated with infections (p = 0.036 and p = 0.013, respectively). Necropsy findings revealed diffuse alveolar damage in the lungs, left ventricular hypertrophy in the heart, liver steatosis and pre-infection arteriosclerosis in the heart and kidneys.
    UNASSIGNED: Our study confirms the main necropsy histopathological findings attributed to COVID-19 in a large patient series, while underlining the importance of both comorbid conditions and superinfections in the pathology.
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  • 文章类型: Journal Article
    BACKGROUND: While the clinical features were described recently, the histopathological characterisation of trunk-dominant canine pemphigus foliaceus (PF) is lacking, and whether it differs from classic facial or insecticide-triggered PF is unknown.
    OBJECTIVE: This study describes the histopathological findings of trunk-dominant PF, and compares the results to classic facial and insecticide-triggered PF.
    METHODS: Skin biopsies from 103 dogs with clinically characterised trunk-dominant (n = 33), classic facial (n = 26) and insecticide-triggered PF (n = 44) were included.
    METHODS: Histological sections, randomised and blinded, were scored for over 50 morphological parameters of pustules, epidermis, dermis, adnexa and crusts. Intact pustule area and width were measured by digital microscopy.
    RESULTS: In trunk-dominant PF, 77 intact pustules were predominantly subcorneal (0.0019-1.940 mm2 area, 0.0470-4.2532 mm wide), and contained from one to over 100 acantholytic keratinocytes. Pustules had boat acantholytic cells, corneocytes, perinuclear eosinophilic rings, neutrophil rosettes, acantholytic cell necrosis, rafts, cling-ons and/or eosinophils. Peripustular epidermal spongiosis, necrosis and lymphocyte exocytosis occurred, as did follicular pustules. Mixed dermal inflammation often contained eosinophils. Trunk-dominant PF did not differ from the other PF groups except for few parameters, such as having fewer rafts (p = 0.003). Additional autoimmune inflammatory patterns occurred in all PF groups.
    CONCLUSIONS: Trunk-dominant PF and other canine PF variants are histologically similar, which indicates shared pathomechanisms. The identification of common boat acantholytic cells and corneocyte separation has implications for the mechanisms of acantholysis. The diversity of histopathological and polyautoimmunity features support complicated immune mechanisms. Finally, results indicate that diagnostic biopsies cannot differentiate between these PF variants in dogs.
    背景: 虽然最近对其临床特征进行了描述,但缺乏躯干显性犬落叶型天疱疮(PF)的组织病理学特征,也不知道它是否与经典的面部或杀虫剂引发的PF不同。 假设/目的: 本研究描述了躯干显性PF的组织病理学发现,并将其结果与经典的面部和杀虫剂触发PF进行了比较。 动物: 包括103只具有临床特征的躯干显性(n=33)、典型面部(n=26)和杀虫剂触发PF(n=44)的犬的皮肤活检。 材料和方法: 组织学切片,随机和盲法,对脓疱、表皮、真皮、毛囊附件和结痂的50多个形态学参数进行评分。通过数字显微镜测量完整的脓疱面积和宽度。 结果: 在躯干显性的PF中,77个完整的脓疱主要位于角质层下(面积0.0019-1.940 mm2 ,宽度0.0470-4.2532 mm),包含1至100多个棘层松懈性角质细胞。脓疱有连片的棘层松懈细胞、角化细胞、核周嗜酸性粒细胞环、中性粒细胞花环、棘层松懈细胞坏死、筏状排列、紧靠和/或嗜酸性粒细胞。出现脓疱周围表皮海绵状水肿、坏死和淋巴细胞外排,毛囊性脓疱也是如此。混合性真皮炎症通常含有嗜酸性粒细胞。躯干显性PF与其他PF组没有差异,除了一些参数,例如筏状排列更少(p=0.003)。所有PF组都出现了额外的自身免疫炎症模式。 结论和临床相关性: 躯干显性PF和其他犬PF变体在组织学上相似,这表明共同的病理机制。常见连片棘层松懈细胞和分离角化细胞的鉴定,牵连出棘层松懈的机制。组织病理学和多重自身免疫特征的多样性支持复杂的免疫机制。最后,结果表明,诊断性活组织检查无法区分犬的这些PF变体。.
    BACKGROUND: Bien que les caractéristiques cliniques aient été décrites récemment, la caractérisation histopathologique du pemphigus foliacé (PF) canin à dominant tronculaire fait défaut, et on ne sait pas s\'il diffère du PF facial classique ou induit par un insecticide. HYPOTHÈSE/OBJECTIFS: Cette étude décrit les résultats histopathologiques provenant de PF à dominante tronculaire et les compare à ceux de PF faciaux classiques et induits par un insecticide. ANIMAUX: les biopsies cutanées de 103 chiens présentant une PF à dominante tronculaire (n = 33), faciale classique (n = 26) et déclenchée par un insecticide (n = 44) sont étudiées. MATÉRIELS ET MÉTHODES: Des coupes histologiques, randomisées et en aveugle, sont évaluées pour plus de 50 paramètres morphologiques relatifs aux pustules, à l\'épiderme, au derme, aux annexes et aux croûtes. La surface et la largeur des pustules intactes sont mesurées par microscopie numérique. RÉSULTATS: Concernant les PF à dominante tronculaire, 77 pustules intactes sont principalement sous-cornéennes (surface de 0,0019 à 1,940 mm2, largeur de 0,0470 à 4,2532 mm) et contiennent de un à plus de 100 kératinocytes acantholytiques. Les pustules contiennent des cellules acantholytiques en radeau, des cornéocytes, des halos éosinophiles périnucléaires, des rosettes de neutrophiles, une nécrose des cellules acantholytiques, des radeaux, des amas et/ou des éosinophiles. Une spongiose épidermique péripustuleuse, une nécrose et une exocytose lymphocytaire sont observées, ainsi que des pustules folliculaires. L\'inflammation cutanée mixte contient souvent des éosinophiles. Le PF à dominante tronculaire ne différe pas des autres groupes de PF, à l’exception de quelques paramètres, comme le fait de présenter moins de radeaux (p = 0,003). D’autres patterns inflammatoires auto-immuns ont été observés dans tous les groupes de PF. CONCLUSIONS ET PERTINENCE CLINIQUE: le PF à dominante tronculaire et les autres variants canins de PF sont histologiquement similaires, ce qui indique des mécanismes pathologiques communs. L\'identification commune de cellules acantholytiques en radeau associée à la disruption des cornéocytes a des implications en termes de mécanisme acantholytique. La diversité des caractéristiques histopathologiques et de polyauto-immunité argue en faveur de mécanismes immunitaires complexes. Enfin, les résultats indiquent que l’examen des biopsies ne permet pas de différencier ces variants de PF chez le chien.
    UNASSIGNED: Während die klinischen Merkmale jüngst beschrieben wurden, fehlt eine histopathologische Charakterisierung des Rumpf-dominierten Pemphigus foliaceus (PF) des Hundes, ebenso ist die Unterscheidung zum klassischen Gesichts- oder Insektizid-ausgelöstem PF unbekannt.
    UNASSIGNED: Diese Studie beschreibt die histopathologischen Befunde des Rumpf-dominierten PF, und vergleicht die Ergebnisse zum klassischen Gesichts- und Insektizid-ausgelösten PF.
    UNASSIGNED: Hautbiopsien von 103 Hunden mit klinisch beschriebenem Rumpf-dominierten PF (n = 33), klassischem Gesichts- (n = 26) und Insektizid-ausgelöstem PF (n = 44) wurden inkludiert.
    UNASSIGNED: Histologische Schnitte, zufällig ausgewählt und geblindet, wurden auf über 50 morphologische Parameter wie Pusteln, Epidermis, Dermis, Adnexe und Krusten untersucht. Bei intakten Pusteln wurde die Stelle und der Durchmesser mittels Digitalmikroskopie gemessen.
    UNASSIGNED: Beim Rumpf-dominierten PF traten intakte Pusteln vor allem subcorneal auf (0,0019-1,949 mm2 ; 0,0470-4,2532 mm Durchmesser) und enthielten zwischen einem und über 100 akantholytische Keratinozyten. Die Pusteln hatten Boot-ähnliche akantholytische Zellen, Korneozyten, perinukleäre eosinophile Ringe, neutrophile Rosetten, akantholytische Zellnekrosen, zusammenhängende Zellen, einzeln anhängende Zellen und/oder Eosinophile. Es trat eine peripustulöse epidermale Spongiose, Nekrose und Lymphozyten Exozytose auf, sowie follikuläre Pusteln. In der gemischten dermalen Entzündung traten auch häufig Eosinophile auf. Der Rumpf-dominierte PF unterschied sich nicht vom PF der anderen Gruppen außer in einigen wenigen Parametern, wie weniger zusammenhängende Zellen (p = 0,003). Zusätzliche autoimmune entzündliche Merkmale traten in allen PF-Gruppen auf.
    UNASSIGNED: Der Rumpf-dominierte PF und andere PF-Varianten des Hundes sind histologisch ähnlich, was einen gemeinsamen Pathomechanismus deutlich macht. Die Identifizierung sogenannter akantholytischer Zellen in Boot-Form und eine Korneozyten Separierung hat auch eine Bedeutung im Bezug auf die Mechanismen der Akantholyse. Die Vielfalt der histopathologischen und polyautoimmunen Merkmale weist auf komplizierte immune Mechanismen hin. Letztendlich zeigen diese Ergebnisse, dass die diagnostischen Biopsien nicht zwischen den PF-Varianten des Hundes unterscheiden können.
    背景: 体幹優位型犬落葉状天疱瘡(PF)の病理組織学的特徴は最近報告されているが、古典的な顔面型や駆虫薬誘発型PFと異なるかどうかは不明である。 仮説・目的: 本研究では、体幹優位型PFの病理組織学的所見を述べ、古典的顔面型PFおよび駆虫薬誘発型PFと比較することであった。 供試動物: 体幹優位型PF(n = 33)、古典的顔面型PF(n = 26)、駆虫薬誘発型PF(n = 44)の臨床的特徴を有する犬103頭の皮膚生検が対象となった。 材料と方法: 組織切片を無作為に盲検化し、膿疱、表皮、真皮、付属器、痂皮の50以上の形態学的パラメータについてスコア化した。膿疱の面積と幅は、デジタル顕微鏡で測定した。 結果: 体幹優位型PFでは、77個の膿疱は主に角層下(面積0.0019-1.940mm2、幅0.0470-4.2532mm)で、1~100個以上の角化細胞が含まれていた。膿疱は、ボート状の棘融解細胞、角質細胞、核周囲の好酸性リング、好中球ロゼット、棘融解細胞壊死、ラフト、クリングオン、好酸球を有していた。毛包の膿疱と同様に、膿疱周囲表皮の海綿状変化、壊死、リンパ球のエクソサイトーシスが起こった。真皮の混合性炎症はしばしば好酸球を含んでいた。体幹優位型PFは、ラフトが少ない(p = 0.003)といったいくつかのパラメータを除いて、他のPFグループと差がなかった。自己免疫性炎症パターンは、すべてのPF群で発生した。 結論と臨床的な関連性: 体幹優位型PFと他の犬PF変種は組織学的に類似しており、これは病態メカニズムを共有していることを示した。共通のボート状棘融解細胞および角質細胞の分離の特定は、棘融解のメカニズムを示唆していた。病理組織学的および多自己免疫学的特徴の多様性は、複雑な免疫機構を支持した。最後に、診断用生検では、犬におけるこれらのPF変種を区別することができないことが示された。.
    UNASSIGNED: Enquanto as características clínicas foram descritas recentemente, ainda falta a caracterização histopatológica do pênfigo foliáceo (PF) canino predominante no tronco e não se sabe se ele difere do PF foliáceo facial clássico e do desencadeado por inseticida. HIPÓTESE/OBJETIVOS: Este estudo descreve os achados histopatológicos do PF predominante no tronco e compara os resultados com o PF clássico e com o desencadeado por inseticidas.
    UNASSIGNED: Foram incluídas biópsias cutâneas de 103 cães com PF caracterizado clinicamente como predominante no tronco (n = 33), facial clássico (n = 26) e desencadeado por inseticidas (n = 44). MATERIAIS E MÉTODOS: Cortes histológicos randomizados e cegos foram classificados por mais de 50 parâmetros morfológicos de pústulas, epiderme, derme, anexos e crostas. A área e a largura das pústulas intactas foram mensuradas por microscopia digital.
    RESULTS: No PF predominante no tronco, as pústulas intactas foram principalmente subcórneas (0,0019-1,940 mm2 de área, 0,0470-4,2532 mm de largura), e continham de um a mais de cem queratinócitos acantolíticos. As pústulas apresentavam células acantolíticas em formato de barco, corneócitos, anéis eosinofílicos perinucleares, rosetas neutrofílicas, necrose de células acantolíticas, aglomerados de células acantolíticas, grupos de células do estrato granuloso aderidas ao estrato córneo (cling-on) e/ou eosinófilos. Ocorreram espongiose epidérmica peripustular, necrose e exocitose de linfócitos, bem como pústulas foliculares. No infiltrado inflamatório dérmico misto frequentemente continha eosinófilos. O PF predominante no tronco não diferiu dos outros grupos de PF exceto para poucos parâmetros, como por exemplo apresentar menos aglomerados de células acantolíticas (p = 0.003). Os outros padrões inflamatórios autoimunes ocorreram em todos os grupos de PF. CONCLUSÕES E RELEVÂNCIA CLÍNICA: O PF predominante no tronco e as outras variantes do PF canino são histologicamente similares, o que indica a existência de patomecanismos comuns. A identificação frequente de células acantolíticas em formato de barco e separação de corneócitos tem implicações nos mecanismos de acantólise. A diversidade as características histopatológicas e poliautoimunes corroboram com os mecanismos imunológicos complexos. Finalmente, os resultados indicam que as biópsias diagnósticas não são capazes de diferenciar essas variantes de PF em cães.
    INTRODUCCIÓN: si bien las características clínicas se describieron recientemente, falta la caracterización histopatológica del pénfigo foliáceo (PF) canino dominante en el tronco, y se desconoce si difiere del PF facial clásico o desencadenado por insecticidas. HIPÓTESIS/OBJETIVOS: Este estudio describe los hallazgos histopatológicos de la PF predominante en el tronco y compara los resultados con PF facial clásico y el PF desencadenado por insecticidas. ANIMALE: Se incluyeron biopsias de piel de 103 perros con PF predominante en el tronco clínicamente caracterizado (n = 33), PF facial clásico (n = 26) y el desencadenado por insecticida (n = 44). MATERIALES Y MÉTODOS: Las secciones histológicas se puntuaron para más de 50 parámetros morfológicos al azar y de forma ciega para las características de pústulas, epidermis, dermis, anejos y costras. El área y el ancho de la pústula intacta se midieron mediante microscopía digital. RESULTADOS: En la PF dominante de tronco 77 pústulas intactas fueron predominantemente subcorneales (0,0019-1,940 mm2 de área, 0,0470-4,2532 mm de ancho) y contenían de uno a más de 100 queratinocitos acantolíticos. Las pústulas tenían células acantolíticas agrupadas, corneocitos, anillos eosinófilos perinucleares, rosetas de neutrófilos, necrosis de células acantolíticas, acantolisis en línea, células acantoliticsas adheridas y/o eosinófilos. Se produjo espongiosis epidérmica peripustulosa, necrosis y exocitosis de linfocitos, así como pústulas foliculares. La inflamación dérmica mixta a menudo contenía eosinófilos. La PF de tronco dominante no se diferenció de los otros grupos de PF excepto por algunos parámetros, como tener menos células en línea (p = 0,003). Se produjeron patrones inflamatorios autoinmunitarios adicionales en todos los grupos de PF. CONCLUSIONES Y RELEVANCIA CLÍNICA: El PF dominante en el tronco y otras variantes del PF canino son histológicamente similares, lo que indica mecanismos patogénicos compartidos. La identificación de células acantolíticas en grupos y la separación de corneocitos tiene implicaciones para los mecanismos de acantólisis. La diversidad de características histopatológicas y de poliautoinmunidad respalda mecanismos inmunitarios complicados. Finalmente, los resultados indican que las biopsias de diagnóstico no pueden diferenciar entre estas variantes de PF en perros.
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  • 文章类型: Journal Article
    目前在巴西和其他国家,鸣禽是非法贩运中最普遍的动物,所以他们经常被没收,这构成了法律,伦理,和保护挑战。将它们归还大自然需要复杂而昂贵的管理,在文献中很少讨论的话题。这里,我们描述了与尝试恢复和释放被没收的鸣禽到野外相关的过程和成本。共有1,721只数种鸣鸟被隔离,康复,并被释放,主要在两个农场位于其典型的地理分布内。对来自370只鸟的样品进行健康评估。血清学显示没有针对新城疫的抗体,和沙门氏菌。文化是阴性的。实时聚合酶链反应在七只鸟的样品中检测到鸡败血症。弓形虫属。和Acuariaspp。感染,脓毒症,创伤是鸟类死亡的首要原因。大约6%的被释放的鸟类被重新捕获,在释放后的平均249天内,距离释放地点平均距离为2,397米。这些鸟类中的大多数是在具有本地或耕地的过渡生态区的片段内或附近发现的自由生活伴侣,和原生树林/森林,和灌木丛。然而,具有丰富的林下再生的桉树人工林为释放的森林物种定居提供了合适的环境,因为他们在这些遗址的防御过程中被重新捕获。超过一半的被重新捕获的鸟类表现出具有显性和驯服特征的行为特征。具有优势性状的鸟类更有可能在栖息地定居,并在野外工作中面对活的诱饵,而具有驯服特征的鸟类倾向于接受与人类的亲密接触。群青长嘴(Cyanoloxiabrissonii),被释放的物种中最不常见的物种,在释放部位,在距释放部位的最短平均距离内显示出几乎2倍的再捕获率。这表明领土竞争减少,也许是鸟类在这里重建的主要因素。每只鸟的总成本为57美元。我们的研究结果表明,被没收的鸣禽在野外有适当的生存和重建,正如我们所描述的那样管理。
    Songbirds are currently the most prevalent animals in illegal trafficking in Brazil and other countries, so they are often confiscated, and this poses legal, ethical, and conservation challenges. Returning them to nature requires complex and expensive management, a topic that is sparingly addressed in the literature. Here, we described the processes and costs associated with an attempt to rehabilitate and release confiscated songbirds into the wild. A total of 1,721 songbirds of several species were quarantined, rehabilitated, and released, primarily on two farms located within their typical geographical distribution. Health assessments were performed on samples from 370 birds. Serology revealed no antibodies against Newcastle disease, and Salmonella spp. cultures were negative. Real-time polymerase chain reactions detected M. gallisepticum in samples from seven birds. Atoxoplasma spp. and Acuaria spp. infections, sepsis, and trauma were the top causes of bird death. About 6% of the released birds were recaptured, within an average period of 249 days after release, and at a mean distance of 2,397 meters from the release sites. The majority of these birds were found with free-living mates within or close to fragments of transitional ecoregions with native or cultivated grasslands, and native groves/forests, and shrublands. However, eucalyptus plantations with rich understory regeneration provided a suitable environment for the released forest species to settle, since they were recaptured during the defense of these sites. Over half of the recaptured birds presented behavioral profiles with both dominant and tame traits. Birds with dominant traits are more likely to settle in habitats and face the live decoys during fieldwork, whereas birds with tame characteristics tend to accept close contact with humans. Ultramarine grosbeak (Cyanoloxia brissonii), the least common species among those released, at the release sites showed an almost 2-fold recapture rate in the shortest mean distances from the release sites. This suggests less territory competition, perhaps a major factor of bird re-establishment here. The total per-bird cost was USD 57. Our findings suggested suitable survival and re-establishment of confiscated songbirds in the wild, when managed as we describe.
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  • 文章类型: Case Reports
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