■到目前为止,全球有超过7.7亿人成为2019年冠状病毒病(COVID-19)的疗养者。新出现的证据强调了COVID-19在急性期和恢复期对口腔微生物组的影响。一线医护人员暴露于病毒感染的风险较高,COVID-19对其口腔微生物组的影响仍未被探索。
■口咽拭子标本,在COVID-19测试阴性后一个月,从一个由55名医护人员组成的队列中收集,进行16SrRNA测序。我们对COVID-19后队列和来自同一参与者的感染前数据集进行了比较分析。社区构成分析,指示物种分析,阿尔法多样性评估,β多样性探索,并对功能预测进行了评估。
■与感染前队列相比,COVID-19后的口腔微生物群落的Shannon和Simpson指数显着下降。此外,两组之间存在明显的组间聚集.在后COVID-19组中,Firmicutes门显示出显着的增加。Further,与感染前相比,几种细菌属的相对丰度有明显差异,包括链球菌,Gemella,肉芽肿,Capnocytophaga,Leptotrichia,梭杆菌,还有Prevotella.我们在COVID-19后组中发现了Gemella富集,可能用作恢复期绩效指标。功能预测显示,后COVID-19组的脂多糖生物合成下调,与宿主炎症反应调节和先天防御机制有关的结果。
■在COVID-19的恢复阶段,一线医护人员的口腔微生物组多样性未能完全恢复到感染前的状态。尽管一个月后COVID-19检测结果呈阴性,口腔微生物群的组成和功能属性存在显著差异.
UNASSIGNED: To date, more than 770 million individuals have become coronavirus disease 2019 (COVID-19) convalescents worldwide. Emerging evidence highlights the influence of COVID-19 on the oral microbiome during both acute and convalescent disease phases. Front-line healthcare workers are at an elevated risk of exposure to viral infections, and the effects of COVID-19 on their oral microbiome remain relatively unexplored.
UNASSIGNED: Oropharyngeal swab specimens, collected one month after a negative COVID-19 test from a cohort comprising 55 healthcare workers, underwent 16S rRNA sequencing. We conducted a comparative analysis between this post-COVID-19 cohort and the pre-infection dataset from the same participants. Community composition analysis, indicator species analysis, alpha diversity assessment, beta diversity exploration, and functional prediction were evaluated.
UNASSIGNED: The Shannon and Simpson indexes of the oral microbial community declined significantly in the post-COVID-19 group when compared with the pre-infection cohort. Moreover, there was clear intergroup clustering between the two groups. In the post-COVID-19 group, the phylum Firmicutes showed a significant increase. Further, there were clear differences in relative abundance of several bacterial genera in contrast with the pre-infection group, including Streptococcus, Gemella, Granulicatella, Capnocytophaga, Leptotrichia, Fusobacterium, and Prevotella. We identified Gemella enrichment in the post-COVID-19 group, potentially serving as a recovery period performance indicator. Functional prediction revealed lipopolysaccharide biosynthesis downregulation in the post-COVID-19 group, an outcome with host inflammatory response modulation and innate defence mechanism implications.
UNASSIGNED: During the recovery phase of COVID-19, the oral microbiome diversity of front-line healthcare workers failed to fully return to its pre-infection state. Despite the negative COVID-19 test result one month later, notable disparities persisted in the composition and functional attributes of the oral microbiota.