PDF(Pubmed)
Abstract：
Biofilm formation in dental unit waterlines and the resulting microbial contamination of the water in the system has become a significant problem. Contaminated water in the dental units is a major concern in dental clinics due to potential risk of causing infections particularly in elderly and immunocompromised patients. The aim of this study was at first to determine microbial contamination of the dental unit waterlines and then to study the efficacy of a comprehensive disinfection protocol on decreasing the microbial load. Water samples were collected before and after disinfection procedure from handpieces and water storage bottles from the dental units, a small 1-cm tubing was cut from each unit and subjected to microbiological culture on different growth media. Identification of the predominant species was achieved by 16S rRNA gene sequencing. Microbial growth was observed in samples collected from all dental units. Upon disinfection procedure, microbial contamination in the water samples and in the tubing surfaces was significantly reduced (P > 0.05). 16S rRNA gene sequencing revealed the presence of several species belonging to the genera Staphylococcus, Corynebacterium and Roseomonas, some of which are implicated in human infections. Aggravation of the biofilm growth on the tubing surfaces and the microbial contamination in the water can be effectively controlled by implementing appropriate and routine disinfection protocols. This may help protect the dental unit staff and the patients being exposed to the risk of infections.
摘要：
牙科单元水线中的生物膜形成和系统中水的所产生的微生物污染已成为显著的问题。由于引起感染的潜在风险,尤其是在老年和免疫受损的患者中,牙科单元中的污染水是牙科诊所中的主要问题。这项研究的目的是首先确定牙科单元水线的微生物污染，然后研究全面消毒方案对降低微生物负荷的功效。在消毒程序之前和之后，从手持件和牙科单元的储水瓶中收集水样，从每个单元上切下一个1厘米的小管子，并在不同的生长培养基上进行微生物培养。通过16SrRNA基因测序实现了优势物种的鉴定。在从所有牙科单元收集的样品中观察到微生物生长。在消毒过程中，水样和油管表面的微生物污染显著减少(P>0.05)。16SrRNA基因测序显示存在几种属于葡萄球菌属的物种，棒状杆菌和玫瑰单胞菌，其中一些与人类感染有关。通过实施适当的和常规的消毒方案,可以有效地控制管道表面上的生物膜生长的加重和水中的微生物污染。这可以帮助保护牙科单元工作人员和暴露于感染风险的患者。
