本研究旨在评估细胞增殖,通过免疫组织化学方法检测犬口腔乳头状瘤病的p53水平和凋亡指数。研究材料包括从六只狗身上采集的肿瘤组织样本,这些狗被动物医学院病理学系录取,卡夫卡斯大学,Kars,蒂尔基耶.免疫组织化学染色选择抗生物素蛋白-生物素过氧化物酶法。犬口腔乳头状瘤病病例,确定是由犬乳头瘤病毒1引起的,被发现具有相当高的细胞增殖指数。此外,所有病例经免疫组化证实携带突变型p53基因.尽管p53基因突变,诊断为肿瘤的狗的Bax/Bcl-2比率的变化有利于促凋亡Bax基因。确定凋亡机制通过胱天蛋白酶依赖性和胱天蛋白酶非依赖性途径发生。虽然在某些情况下病变占据了整个口腔,组织病理学,在6例中均未发现恶性转化。
This study was aimed at the evaluation of cell proliferation, p53 level and apoptotic index by immunohistochemical methods in canine oral
papillomatosis. The study material comprised of tumor tissue samples taken from six dogs being admitted to the Pathology Department of Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Cases of canine oral
papillomatosis, determined to have been caused by canine papillomavirus-1, were found to have a rather high cell proliferation index. Furthermore, all cases were immunohisto-chemically demonstrated to carry a mutant p53 gene. Despite the mutation of p53 gene, the shift in the Bax/Bcl-2 ratio of dogs diagnosed with tumor was in favor of the pro-apoptotic Bax gene. The apoptotic mechanism was determined to occur through both the caspase-dependent and caspase-independent pathways. While the lesions occupied the entire oral cavity in some cases, histopathologically, malignant transformation was not detected in any of the six cases.