■在石油和天然气管道内,天然微生物群落和不同的固体化合物通常共存并形成混合沉积物。然而,这些沉积物(主要由矿物相组成)与油气系统中的微生物之间的相互作用仍然知之甚少。这里,我们研究了磁铁矿(Fe3O4)的影响,三叶岩(FeS),和二氧化硅(SiO2)对微生物多样性的影响,细胞活力,生物膜的形成,和EPS组成的石油回收多物种财团。
■在单独的生物反应器中培养油田回收的微生物群落2周,每个包含10克市售磁铁矿(Fe3O4),三叶岩(FeS),或在40°C±1°C下在20%CO2/80%N2的气体气氛下的二氧化硅(SiO2)。
■三叶岩中形成的微生物种群与二氧化硅和磁铁矿中形成的微生物种群明显不同,表现出显著的相似性。软岩中的优势类群是脱硫磺基弧菌属,而硫磺螺旋菌在磁铁矿和二氧化硅中占主导地位。然而,生物膜的形成在三叶岩上最低,在二氧化硅上最高,与观察到的细胞活力相关。
■三绿岩的溶解,然后将HS-(H2S)和Fe2释放到测试溶液中,与二氧化硅相比,其粒径更大,可能有助于观察到的结果。共聚焦激光扫描显微镜显示,在二氧化硅中形成的生物膜的EPS以eDNA为主,而三叶草和磁铁矿中的主要含有多糖。虽然这种现象的机制还不能确定,预计这些发现对于增强目前在石油和天然气系统中使用的MIC缓解策略特别有价值。
UNASSIGNED: Inside oil and gas pipelines, native microbial communities and different solid compounds typically coexist and form mixed deposits. However, interactions between these deposits (primarily consisting of mineral phases) and microorganisms in oil and gas systems remain poorly understood. Here, we investigated the influence of magnetite (Fe3O4), troilite (FeS), and silica (SiO2) on the microbial diversity, cell viability, biofilm formation, and EPS composition of an oil-recovered multispecies consortium.
UNASSIGNED: An oilfield-recovered microbial consortium was grown for 2 weeks in separate bioreactors, each containing 10 g of commercially available magnetite (Fe3O4), troilite (FeS), or silica (SiO2) at 40°C ± 1°C under a gas atmosphere of 20% CO2/80% N2.
UNASSIGNED: The microbial population formed in troilite significantly differed from those in silica and magnetite, which exhibited significant similarities. The dominant taxa in troilite was the Dethiosulfovibrio genus, whereas Sulfurospirillum dominated in magnetite and silica. Nevertheless, biofilm formation was lowest on troilite and highest on silica, correlating with the observed cell viability.
UNASSIGNED: The dissolution of troilite followed by the liberation of HS- (H2S) and Fe2+ into the test solution, along with its larger particle size compared to silica, likely contributed to the observed results. Confocal laser scanning microscopy revealed that the EPS of the biofilm formed in silica was dominated by eDNA, while those in troilite and magnetite primarily contained polysaccharides. Although the mechanisms of this phenomenon could not be determined, these findings are anticipated to be particularly valuable for enhancing MIC mitigation strategies currently used in oil and gas systems.