背景:囊胚,一种广泛分布的人畜共患原生动物,感染人类和许多动物,对其潜在的医学和兽医意义知之甚少。这项研究调查了孟加拉国儿童和小牛囊胚的分子发生和遗传变异,以探索跨物种传播和疾病负担。
方法:总共,调查了998个DNA样本,包括来自儿童的299种粪便DNA和来自小牛的699种粪便DNA,使用聚合酶链反应和小亚基核糖体RNA(SSUrRNA)基因的测序。
结果:本研究在5.35%的儿童和14.74%的小牛中检测到胚泡。虽然在不同的研究变量中观察到发生率略有差异,无统计学意义。在5岁以下儿童和1-3个月的小牛中发生率最高。关于品种,荷斯坦弗利西亚十字架和泽西十字架的感染率更高。相反,Gazipur地区的儿童和小牛发生率较低。其余参数显示几乎相等的囊胚百分比。在儿童中鉴定的亚型包括ST1、ST3和ST4,其中ST1占其中的50%。在小牛中也发现了ST3和ST4,ST10(55.34%)是最普遍的。在小牛中发现的其他亚型是ST14,ST21和ST24-ST26。
结论:这项研究发现,与孟加拉国的儿童相比,小牛中的囊胚病更常见,具有九种亚型的遗传多样性。在两个种群中常见的两种亚型的相同变异表明潜在的人畜共患传播,强调必须在“一个健康”框架内进行进一步的分子调查和综合措施,以减轻公共卫生风险。
BACKGROUND: Blastocystis, a widely distributed zoonotic protozoan infecting both humans and numerous animals, remains poorly understood with its potential medical and veterinary significance. This study examined the molecular
occurrence and genetic variation of Blastocystis in children and calves in Bangladesh to explore cross-species transmission and disease burden.
METHODS: In total, 998 DNA samples were investigated, comprising 299 stool DNA from children and 699 fecal DNA from calves, using polymerase chain reaction and sequencing of the small subunit ribosomal RNA (SSU rRNA) gene.
RESULTS: This study detected Blastocystis in 5.35% of the children and 14.74% of the calves. While slight variations in
occurrence rates were observed across different study variables, none were statistically significant. The
occurrence was highest among children under 5 years and calves aged 1-3 months. Regarding breed, the Holstein Friesian cross and the Jersey cross exhibited higher rates of infection. Conversely, occurrences were lower among children and calves in Gazipur district. The remaining parameters displayed nearly equivalent percentages of Blastocystis. The subtypes identified in children included ST1, ST3, and ST4, with ST1 comprising 50% of them. ST3 and ST4 were also found in calves, alongside ST10 (55.34%) being the most prevalent. Other subtypes found in calves were ST14, ST21, and ST24-ST26.
CONCLUSIONS: This study found that Blastocystis is more common in calves than in children in Bangladesh, with genetic diversity of nine subtypes. The common
occurrence of identical variants of two subtypes in both populations suggests potential zoonotic transmission, highlighting the necessity for further molecular investigations and comprehensive measures within the One Health framework to mitigate public health risks.