我们使用Lenstar900(LS900)研究眼睛的尺寸和形状,A-扫描超声检查,高度近视猕猴的磁共振成像(MRI)。从8只高度近视(≤-1000DS)的眼睛(4只成年雄性食蟹猴)评估了LS900,A扫描超声和MRI的眼睛尺寸数据,并通过一致系数和95%一致界限进行了比较。进行多元回归分析以探讨眼生物测量之间的关联,volume,折射和仪器间的差异。在两个时间点的三个眼部参数测量的测试-重测可靠性几乎相等(组内相关性=0.831至1.000)。对于玻璃体腔深度(VCD),三个测量的平行形式可靠性很强(一致系数=0.919至0.981),中等轴向长度(AL)(一致系数=0.486至0.981),前房深度(ACD)(一致系数=0.267至0.621)和晶状体厚度(LT)(一致系数=0.035和0.631)较弱。与A扫描超声相比,LS900和MRI系统地低估了ACD和LT(P<0.05)。值得注意的是,LS900的平均AL与MRI(r=0.978,P<0.001)和A扫描超声(r=0.990,P<0.001)显着相关。几乎有4/5的眼球扩张。平均眼球体积与AL呈正相关(r=0.782,P=0.022)。宽度(r=0.945,P=0.000),眼球长度(r=0.782,P=0.022),与SER呈负相关(r=-0.901,P=0.000)。总之,VCD与LS900,A扫描超声和MRI的仪器间一致性很高,虽然与A扫描超声相比,LS900的ACD和LT被低估了,LS900和A扫描超声可以可靠地测量AL。MRI进一步揭示了极端近视的非人灵长类动物的赤道球形。
We investigate the ocular dimensions and shape by using Lenstar900 (LS900), A-scan ultrasonography, and Magnetic Resonance Imaging (MRI) in highly myopic Macaca fascicularis. The ocular dimensions data of LS900, A-scan ultrasonography and MRI was assessed from 8 eyes (4 adult male cynomolgus macaque) with extremely high myopia (≤-1000DS) and compared by means of coefficients of concordance and 95% limits of agreement. Multiple regression analysis was performed to explore the associations between ocular biometry, volume, refraction and inter-instrument discrepancies. Test-retest reliability of three measurements of ocular parameters at two time points was almost equal (intraclass correlation = 0.831 to 1.000). The parallel-forms reliability of three measurements was strong for vitreous chamber depth (VCD) (coefficient of concordance = 0.919 to 0.981), moderate for axial length (AL) (coefficient of concordance = 0.486 to 0.981), and weak for anterior chamber depth (ACD) (coefficient of concordance = 0.267 to 0.621) and lens thickness (LT) (coefficient of concordance = 0.035 to 0.631). The LS900 and MRI systematically underestimated the ACD and LT comparing to A-scan ultrasonography (P < 0.05). Notably, the average AL on LS900 displayed a significant correlation with those on MRI (r = 0.978, P < 0.001) and A-scan ultrasonography (r = 0.990, P < 0.001). Almost 4/5 eyeballs were prolate. The mean eyeball volume positively correlated with AL (r = 0.782, P = 0.022), the width (r = 0.945, P = 0.000), and the length (r = 0.782, P = 0.022) of eyeball, while negatively correlated with SER (r = -0.901, P = 0.000). In conclusion, there was a high inter-instrument concordance for VCD with LS900, A-scan ultrasonography and MRI, while ACD and LT were underestimated with LS900 compared to A-scan ultrasonography, and the LS900 and A-scan ultrasonography could reliably measure the AL. MRI further revealed an equatorial globe shape in extremely myopic non-human primates.