电纺纳米纤维在纳米结构材料的合成中表现出巨大的潜力,从而为增强伤口护理的功效提供了有希望的途径。本研究旨在探讨两种生物大分子的伤口愈合潜力,PCL-明胶纳米纤维粘附骨髓间充质干细胞(BMSCs)。纳米纤维的表征显示平均纤维直径为200至300nm,具有对应于聚己内酯(PCL)和明胶的独特元素峰。此外,骨髓来源的BMSCs整合到纳米纤维中,通过体外和体内方法系统地评估了它们的伤口再生潜力。体外评估证实,掺入BMSC的纳米纤维增强了细胞活力和关键的细胞过程,如粘附,和扩散。随后,进行体内研究以证明纳米纤维的伤口愈合功效。观察到掺入纳米纤维的BMSCs的伤口愈合率超过了这两者,纳米纤维和单独的BMSCs。此外,组织形态学分析显示,加入纳米纤维组的BMSCs加速了再上皮化和改善了伤口收缩。与BMSC结合的制造的纳米纤维在动物模型中表现出优异的伤口再生,并且可以用作伤口愈合贴片。
Electrospun
nanofibers exhibit a significant potential in the synthesis of nanostructured materials, thereby offering a promising avenue for enhancing the efficacy of wound care. The present study aimed to investigate the wound-healing potential of two biomacromolecules, PCL-Gelatin nanofiber adhered with bone marrow-derived mesenchymal stem cells (BMSCs). Characterisation of the nanofiber revealed a mean fiber diameter ranging from 200 to 300 nm, with distinctive elemental peaks corresponding to polycaprolactone (PCL) and gelatin. Additionally, BMSCs derived from bone marrow were integrated into
nanofibers, and their wound-regenerative potential was systematically evaluated through both in-vitro and in-vivo methodologies. In-vitro assessments substantiated that BMSC-incorporated
nanofibers enhanced cell viability and crucial cellular processes such as adhesion, and proliferation. Subsequently, in-vivo studies were performed to demonstrate the wound-healing efficacy of
nanofibers. It was observed that the rate of wound healing of BMSCs incorporated
nanofibers surpassed both, nanofiber and BMSCs alone. Furthermore, histomorphological analysis revealed accelerated re-epithelization and improved wound contraction in BMSCs incorporated nanofiber group. The fabricated nanofiber incorporated with BMSCs exhibited superior wound regeneration in animal model and may be utilised as a wound healing patch.