近年来,包裹的微纳米气泡由于其值得称赞的稳定性而在研究中引起了极大的关注,生物相容性,和其他值得注意的属性。目前,包封微纳米气泡的制备方法具有制备工艺复杂等局限性,大气泡尺寸,分布范围广,低产量,等。迫切需要设计一种简单有效的方法来制备包封的微纳米气泡,确保高浓度和均匀的粒度分布。磁性脂质气泡(MLB)是一种多功能类型的包封微纳米气泡,将磁性纳米颗粒与脂质包被的气泡结合在一起。在这项研究中,MLB是通过基于在交变磁场中热生效应引起的氧化铁纳米颗粒的界面自组装的磁性内部热泡生成过程来简单有效地制备的。获得的MLBs的平均流体动力学直径为384.9±8.5nm,多分散指数(PDI)为0.248±0.021,ζ电位为-30.5±1.0mV,浓度为(7.92±0.46)×109个气泡/mL。电子显微镜结果表明,MLB具有规则的球形稳定的核-壳结构。超顺磁性氧化铁纳米颗粒(SPIONs)和磷脂层吸附在MLBs的球形气核周围,导致粒子表现出值得称赞的超顺磁性和磁性。此外,工艺参数对MLB形态的影响,包括磷脂浓度,磷脂比例,电流强度,磁热时间,和SPION浓度,进行了研究和讨论,以实现MLBs的控制制备。体外成像结果表明,负载氧化铁纳米颗粒的MLBs浓度越高,体外超声(US)成像和磁共振成像(MRI)结果越好。本研究证明,磁内热泡生成过程是制备高浓度MLBs的一种简单高效的技术,规则结构,和值得称赞的财产。这些发现为包裹微纳米气泡的大规模生产和应用奠定了坚实的基础。特别是在生物医学领域和其他相关领域。
In recent years, enveloped micro-
nanobubbles have garnered significant attention in research due to their commendable stability, biocompatibility, and other notable properties. Currently, the preparation methods of enveloped micro-
nanobubbles have limitations such as complicated preparation process, large bubble size, wide distribution range, low yield, etc. There exists an urgent demand to devise a simple and efficient method for the preparation of enveloped micro-
nanobubbles, ensuring both high concentration and a uniform particle size distribution. Magnetic lipid bubbles (MLBs) are a multifunctional type of enveloped micro-nanobubble combining magnetic nanoparticles with lipid-coated bubbles. In this study, MLBs are prepared simply and efficiently by a magneto internal heat bubble generation process based on the interfacial self-assembly of iron oxide nanoparticles induced by the thermogenic effect in an alternating magnetic field. The mean hydrodynamic diameter of the MLBs obtained was 384.9 ± 8.5 nm, with a polydispersity index (PDI) of 0.248 ± 0.021, a zeta potential of -30.5 ± 1.0 mV, and a concentration of (7.92 ± 0.46) × 109 bubbles/mL. Electron microscopy results show that the MLBs have a regular spherical stable core-shell structure. The superparamagnetic iron oxide nanoparticles (SPIONs) and phospholipid layers adsorbed around the spherical gas nuclei of the MLBs, leading the particles to demonstrate commendable superparamagnetic and magnetic properties. In addition, the effects of process parameters on the morphology of MLBs, including phospholipid concentration, phospholipid proportiona, current intensity, magnetothermal time, and SPION concentration, were investigated and discussed to achieve controlled preparation of MLBs. In vitro imaging results reveal that the higher the concentration of MLBs loaded with iron oxide nanoparticles, the better the in vitro ultrasound (US) imaging and magnetic resonance imaging (MRI) results. This study proves that the magneto internal heat bubble generation process is a simple and efficient technique for preparing MLBs with high concentration, regular structure, and commendable properties. These findings lay a robust foundation for the mass production and application of enveloped micro-
nanobubbles, particularly in biomedical fields and other related domains.