开花时间对于生存和繁殖至关重要。李属属于玫瑰科,包括数百种开花树木和灌木,具有重要的观赏和经济价值。然而,李属早期开花的分子机制尚不清楚。这里,我们利用梅花和桃花的基因组和转录组探索早期开花的转录调控机制。比较基因组学发现,占总P.mu基因组92.4%和总P.serica基因组91.2%的基因属于正统。在P.mume和P.persica之间共发现19,169个正统,包括20,431个相应的直系同源物和20,080个共线性基因对。共发现305个与早期开花相关的差异表达基因(DEGs),其中FT,TLI65和NAP57被鉴定为早期开花调节途径中的枢纽基因。此外,我们从9个蛋白质家族中鉴定出25个转录因子(TFs),包括MADS-box,AP2/ERF,还有MYB.我们的结果提供了对李属开花时间调节的潜在分子模型的见解,并强调了多组学在破译属间植物特性中的实用性。
Flowering time is crucial for the survival and reproduction. Prunus genus belongs to the Rosaceae family and includes several hundred species of flowering trees and shrubs with important ornamental and economic values. However, the molecular mechanism underlying early flowering in Prunus genus is unclear. Here, we utilized the genome and transcriptome of P. mume and P. persica to explore the transcriptional regulation mechanism of early flowering. Comparative genomics found that genes accounting for 92.4% of the total P. mume genome and 91.2% of the total P. persica genome belonged to orthogroups. A total of 19,169 orthogroups were found between P. mume and P. persica, including 20,431 corresponding orthologues and 20,080 collinearity gene pairs. A total of 305 differentially expressed genes (DEGs) associated with early flowering were found, among which FT, TLI65, and NAP57 were identified as hub genes in the early flowering regulation pathway. Moreover, we identified twenty-five transcription factors (TFs) from nine protein families, including MADS-box, AP2/ERF, and MYB. Our results provide insights into the underlying molecular model of flowering time regulation in Prunus genus and highlight the utility of multi-omics in deciphering the properties of the inter-genus plants.