伯内蒂柯西拉,人类Q热和动物考西病的病原体,是一种人畜共患的传染性细菌,具有复杂的生态学,这源于其在多个(in)脊椎动物宿主物种中复制的能力。西班牙每年向ECDC通报最多的Q发烧病例,野生动植物在该国的C.burnetii生态中起着重要作用。然而,Burnetii宿主的全貌是不完整的,因此,这项研究旨在更好地了解微型哺乳动物在该国C.burnetii生态中的作用。通过qPCR分析了来自10个物种的816只小型哺乳动物的脾脏样品和来自小虫鼠的130只阴道拭子,以检测C.burnetii感染和脱落,分别。9.7%的脾样品为qPCR阳性。感染率最高(10.8%)的是小白鼠,其中在分析的130个阴道拭子中的1个(0.8%)中也检测到了C.burnetiiDNA。也发现了阳性的样本在Apodemussylvaticus(8.7%),红豆杉(7.7%)和家鼠(6.4%)。通过PCR与反向线印迹的耦合对阳性样品进行基因分型,并且在来自M.arvalis的阳性样品之一中首次鉴定出基因型II菌株,而其余样品只能获得部分结果。参与研究的研究人员之一诊断出急性Q热,这可能与Arvalis的处理有关。该研究的结果与先前的发现一致,表明微哺乳动物可以被C.burnetii感染。我们的发现还表明,微哺乳动物可能是追溯欧洲人类Q热和动物柯西氏菌病病例起源的潜在来源。
Coxiella burnetii, the causal agent of human Q fever and animal Coxiellosis, is a zoonotic infectious bacterium with a complex ecology that results from its ability to replicate in multiple (in)vertebrate host species. Spain notifies the highest number of Q fever cases to the ECDC annually and wildlife plays a relevant role in C. burnetii ecology in the country. However, the whole picture of C. burnetii hosts is incomplete, so this study seeks to better understand the role of
micromammals in C. burnetii ecology in the country. Spleen samples from 816
micromammals of 10 species and 130 vaginal swabs from Microtus arvalis were analysed by qPCR to detect C. burnetii infection and shedding, respectively. The 9.7% of the spleen samples were qPCR positive. The highest infection prevalence (10.8%) was found in Microtus arvalis, in which C. burnetii DNA was also detected in 1 of the 130 vaginal swabs (0.8%) analysed. Positive samples were also found in Apodemus sylvaticus (8.7%), Crocidura russula (7.7%) and Rattus rattus (6.4%). Positive samples were genotyped by coupling PCR with reverse line blotting and a genotype II+ strain was identified for the first time in one of the positive samples from M. arvalis, whereas only partial results could be obtained for the rest of the samples. Acute Q fever was diagnosed in one of the researchers that participated in the study, and it was presumably linked to M. arvalis handling. The results of the study are consistent with previous findings suggesting that
micromammals can be infected by C. burnetii. Our findings additionally suggest that
micromammals may be potential sources to trace back the origin of human Q fever and animal Coxiellosis cases in Europe.