关键词: ancient DNA micromammals palaeogenetics small mammals

来  源:   DOI:10.3390/genes8110312   PDF(Sci-hub)   PDF(Pubmed)

Abstract:
The field of ancient DNA (aDNA) has recently been in a state of exponential growth, largely driven by the uptake of Next Generation Sequencing (NGS) techniques. Much of this work has focused on the mammalian megafauna and ancient humans, with comparatively less studies looking at micromammal fauna, despite the potential of these species in testing evolutionary, environmental and taxonomic theories. Several factors make micromammal fauna ideally suited for aDNA extraction and sequencing. Micromammal subfossil assemblages often include the large number of individuals appropriate for population level analyses, and, furthermore, the assemblages are frequently found in cave sites where the constant temperature and sheltered environment provide favourable conditions for DNA preservation. This review looks at studies that include the use of aDNA in molecular analysis of micromammal fauna, in order to examine the wide array of questions that can be answered in the study of small mammals using new palaeogenetic techniques. This study highlights the bias in current aDNA studies and assesses the future use of aDNA as a tool for the study of micromammal fauna.
摘要:
古DNA(aDNA)领域最近处于指数增长状态,很大程度上是由下一代测序(NGS)技术的吸收驱动的。这项工作大部分集中在哺乳动物大型动物和古代人类上,对微哺乳动物动物的研究相对较少,尽管这些物种在进化测试中具有潜力,环境和分类学理论。几个因素使微哺乳动物动物区系非常适合aDNA提取和测序。微哺乳动物亚化石组合通常包括适合于种群水平分析的大量个体,and,此外,这些组合经常出现在洞穴中,那里的恒温和庇护环境为DNA保存提供了有利条件。这篇综述着眼于包括在微哺乳动物动物的分子分析中使用aDNA的研究,为了研究使用新的古遗传学技术在小型哺乳动物研究中可以回答的广泛问题。这项研究强调了当前aDNA研究中的偏见,并评估了aDNA作为研究微哺乳动物动物群的工具的未来用途。
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