目的:MiRSNP可能通过影响miRNA结合位点的直接变化或影响该区域的二级结构以及该区域对miRNA的可接近性的变化,通过影响microRNAs(miRNAs)-mRNA相互作用来干扰mRNA稳定性。研究证实,白细胞介素-15受体α(IL-15RA)水平升高在系统性红斑狼疮(SLE)和类风湿性关节炎(RA)的发病机制中具有重要作用。在本研究中,第一次,我们的目的是评估miRSNP之间可能的相关性,rs2296135,在IL-15RA基因中具有SLE和RA的风险。
方法:在本病例对照研究中,100例SLE患者,100例RA患者,110名健康参与者被纳入使用实时PCR高分辨率熔解方法评估rs2296135基因型。
结果:根据我们的发现,rs2296135的AA基因型和A等位基因与RA的风险增加显著相关(对于AA基因型,OR=2.29;95%CI[1.06-5.02];对于A等位基因,OR=1.65;95%CI[1.10-2.48])。然而,这一常见变异与研究人群的SLE风险无显著相关.RA组的分层分析证实,A等位基因患者的血清C反应蛋白(CRP)浓度明显更高(P<0.001)。在SLE科目中,A等位基因患者的关节炎频率(P:0.021)和肾脏受累频率(P:0.025)明显高于其他SLE个体.
结论:本研究提出IL-15RA基因rs2296135多态性与RA和SLE患者的某些临床特征之间存在显著关联。
OBJECTIVE: MiRSNPs may interfere with mRNA stability through effects on microRNAs (miRNAs)-mRNA interactions via direct changes in miRNA binding site or effect on the secondary structure of this region and changes in accessibility of this region to miRNAs. Studies have confirmed that an elevated level of interleukin-15 receptor alpha (IL-15RA) has an important role in the pathogenesis of systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). In the present study, for the first time, we aimed to evaluate the possible correlation between a miRSNP, rs2296135, in IL-15RA gene with the risk of SLE and RA.
METHODS: In this case-control study, 100 SLE patients, 100 RA patients, and 110 healthy participants were enrolled to assess rs2296135 genotypes with real-time PCR high-resolution melting method.
RESULTS: According to our findings, AA genotype and A allele of rs2296135 were considerably associated with enhanced risk of RA (for AA genotype, OR = 2.29; 95% CI [1.06-5.02]; for A allele, OR = 1.65; 95% CI [1.10-2.48]). However, this common variant was not significantly correlated with SLE risk in population under study. Stratification analysis in the RA group verified that patients with the A allele had considerably higher serum concentrations of C-reactive protein (CRP) (P < 0.001). In SLE subjects, the frequency of arthritis (P: 0.021) and renal involvement (P: 0.025) in patients with A allele was significantly higher than in other SLE individuals.
CONCLUSIONS: The current study proposes a substantial association between rs2296135 polymorphism in IL-15RA gene with augmented risk of RA and some clinical characteristics in RA and SLE patients.