Abstract：
Aim: The study aims to identify high-impact single nucleotide polymorphisms (SNPs) in miRNA target sites of genes associated with lung cancer. Materials & methods: Lung cancer genes were obtained from Uniprot KB. miRNA target site SNPs were mined from MirSNP, miRdSNP and TargetScan. SNPs were shortlisted based on binding impact, minor allele frequency and conservation. Gene expression was analyzed in genes with high-impact SNPs in healthy versus lung cancer tissue. Additionally, enrichment, pathway and network analyzes were performed. Results: 19 high-impact SNPs were identified in miRNA target sites of lung cancer-associated genes. These SNPs affect miRNA binding and gene expression. The genes are involved in key cancer related pathways. Conclusion: The identified high-impact miRNA target site SNPs and associated genes provide a starting point for case-control studies in lung cancer patients in different populations.
[Box: see text].
[Box: see text].
摘要：
目的:研究肺癌相关基因miRNA靶位点中高影响的单核苷酸多态性(SNPs)。材料和方法:肺癌基因获自UniprotKB。miRNA靶位点SNP来自MirSNP，miRdSNP和TargetScan。SNP根据结合影响入围，次要等位基因频率和保守性。在健康与肺癌组织中分析具有高影响SNP的基因中的基因表达。此外,富集,进行了路径和网络分析。结果：在肺癌相关基因的miRNA靶位点中鉴定出19个高影响的SNP。这些SNP影响miRNA结合和基因表达。这些基因参与关键的癌症相关途径。结论：鉴定的高影响miRNA靶位点SNP和相关基因为不同人群肺癌患者的病例对照研究提供了起点。
[方框：见正文]。
[方框：见正文]。
