■双氢青蒿素(DHA),从传统中草药黄花蒿中提取的青蒿素衍生物,具有抑制头颈部鳞状细胞癌(HNSCC)进展的潜力。然而,这些效应的潜在机制尚不清楚.因此,我们旨在研究DHA对肿瘤侵袭和迁移影响的潜在机制。
■将人HNSCC细胞系CAL-27和FaDu暴露于不同浓度的DHA(0、5、20和80μM)24小时。入侵,使用CCK8、Transwell、和伤口愈合试验,分别。实时定量PCR,西方印迹,和免疫荧光用于评估靶基因和蛋白质的表达水平。
■DHA抑制CAL-27和FaDu细胞的侵袭和迁移。此外,miR-195-5p抑制HNSCC细胞的侵袭和迁移。这项研究揭示了临床样本和多个公共数据库之间miR-195-5p和TENM2表达的显著差异。DHA处理和miR-195-5p过表达显著降低了HNSCC细胞中的TENM2表达,这表明miR-195-5p过表达增强了DHA对TENM2的抑制作用。
■这项研究提供了第一个证据,证明DHA通过调节HNSCC细胞中的miR-195-5p/TENM2轴来抑制细胞侵袭和迁移,表明它是HNSCC的潜在有效治疗策略。
UNASSIGNED: Dihydroartemisinin (DHA), an artemisinin derivative extracted from the traditional Chinese medicinal herb Artemisia annua, has the potential to suppress head and neck squamous cell carcinoma (HNSCC) progression. However, the mechanisms underlying these effects remain unclear. Therefore, we aimed to examine the mechanisms underlying the effects of DHA on tumor invasion and migration.
UNASSIGNED: Human HNSCC cell lines CAL-27 and FaDu were exposed to varying DHA concentrations (0, 5, 20, and 80 μM) for 24 h. Cell proliferation, invasion, and migration were assessed using CCK8, transwell, and wound-healing assays, respectively. Quantitative real-time PCR, western blotting, and immunofluorescence were used to assess the expression levels of the target genes and proteins.
UNASSIGNED: DHA suppressed the invasion and migration of CAL-27 and FaDu cells. Additionally, miR-195-5p suppressed the invasion and migration of HNSCC cells. This study revealed significant differences in the expression of miR-195-5p and TENM2 between clinical samples and multiple public databases. DHA treatment and miR-195-5p overexpression significantly reduced TENM2 expression in HNSCC cells, which suggested that miR-195-5p overexpression enhanced the inhibitory effect of DHA on TENM2.
UNASSIGNED: This study provides the first evidence that DHA inhibits cell invasion and migration by regulating the miR-195-5p/TENM2 axis in HNSCC cells, suggesting it as a potentially effective treatment strategy for HNSCC.