METHODS: This study involved 112 CAS patients and 65 healthy individuals. Serum miR-195-5p levels were measured using RT-qPCR. The ROC curve was then plotted to evaluate the diagnostic potential of miR-195-5p for CAS. The Kaplan-Meier curve and Cox regression were employed to determine miR-195-5p\'s prognostic significance. In vitro, the effects of miR-195-5p mimic or inhibitor on VSMC proliferation and migration were assessed using CCK-8 and Transwell assays.
RESULTS: In CAS patients, serum miR-195-5p levels were elevated and correlated with the degree of CAS. The ROC curve had an AUC value of 0.897, with sensitivity of 71.4% and specificity of 95.4%. Higher levels of miR-195-5p indicated a higher risk of CIE occurrence and may serve as an independent predictor of CIE. The upregulation of miR-195-5p promoted VSMC proliferation and migration, while downregulation had the opposite effect.
CONCLUSIONS: miR-195-5p was demonstrated to have diagnostic and prognostic significance in CAS and may serve as a potential biomarker. It may contribute to the progression of CAS by promoting the proliferation and migration of VSMCs.
方法:本研究涉及112名CAS患者和65名健康个体。使用RT-qPCR测量血清miR-195-5p水平。然后绘制ROC曲线以评估miR-195-5p对CAS的诊断潜力。采用Kaplan-Meier曲线和Cox回归来确定miR-195-5p的预后意义。体外,使用CCK-8和Transwell试验评估了miR-195-5p模拟物或抑制剂对VSMC增殖和迁移的影响.
结果:在CAS患者中,血清miR-195-5p水平升高,且与CAS程度相关。ROC曲线的AUC值为0.897,敏感性为71.4%,特异性为95.4%。较高水平的miR-195-5p表明CIE发生的风险较高,可能是CIE的独立预测因子。miR-195-5p上调促进VSMC增殖和迁移,而下调则产生了相反的效果。
结论:miR-195-5p被证明在CAS中具有诊断和预后意义,并可能作为潜在的生物标志物。它可能通过促进VSMC的增殖和迁移来促进CAS的发展。