Abstract：
OBJECTIVE: Carotid artery stenosis (CAS) is a leading cause of cerebral ischemic events (CIE). Timely detection and risk assessment can aid in managing CAS patients and improving their prognosis. The aim of the current study is to identify a new biomarker for CAS and to further investigate the impact of miR-195-5p on cellular processes in vascular smooth muscle cells (VSMCs).
METHODS: This study involved 112 CAS patients and 65 healthy individuals. Serum miR-195-5p levels were measured using RT-qPCR. The ROC curve was then plotted to evaluate the diagnostic potential of miR-195-5p for CAS. The Kaplan-Meier curve and Cox regression were employed to determine miR-195-5p\'s prognostic significance. In vitro, the effects of miR-195-5p mimic or inhibitor on VSMC proliferation and migration were assessed using CCK-8 and Transwell assays.
RESULTS: In CAS patients, serum miR-195-5p levels were elevated and correlated with the degree of CAS. The ROC curve had an AUC value of 0.897, with sensitivity of 71.4% and specificity of 95.4%. Higher levels of miR-195-5p indicated a higher risk of CIE occurrence and may serve as an independent predictor of CIE. The upregulation of miR-195-5p promoted VSMC proliferation and migration, while downregulation had the opposite effect.
CONCLUSIONS: miR-195-5p was demonstrated to have diagnostic and prognostic significance in CAS and may serve as a potential biomarker. It may contribute to the progression of CAS by promoting the proliferation and migration of VSMCs.
METHODS: This study involved 112 CAS patients and 65 healthy individuals. Serum miR-195-5p levels were measured using RT-qPCR. The ROC curve was then plotted to evaluate the diagnostic potential of miR-195-5p for CAS. The Kaplan-Meier curve and Cox regression were employed to determine miR-195-5p\'s prognostic significance. In vitro, the effects of miR-195-5p mimic or inhibitor on VSMC proliferation and migration were assessed using CCK-8 and Transwell assays.
RESULTS: In CAS patients, serum miR-195-5p levels were elevated and correlated with the degree of CAS. The ROC curve had an AUC value of 0.897, with sensitivity of 71.4% and specificity of 95.4%. Higher levels of miR-195-5p indicated a higher risk of CIE occurrence and may serve as an independent predictor of CIE. The upregulation of miR-195-5p promoted VSMC proliferation and migration, while downregulation had the opposite effect.
CONCLUSIONS: miR-195-5p was demonstrated to have diagnostic and prognostic significance in CAS and may serve as a potential biomarker. It may contribute to the progression of CAS by promoting the proliferation and migration of VSMCs.
摘要：
目的：颈动脉狭窄（CAS）是脑缺血事件（CIE）的主要原因。及时发现和风险评估可以帮助管理CAS患者并改善其预后。本研究的目的是确定CAS的新生物标志物，并进一步研究miR-195-5p对血管平滑肌细胞（VSMC）细胞过程的影响。
方法：本研究涉及112名CAS患者和65名健康个体。使用RT-qPCR测量血清miR-195-5p水平。然后绘制ROC曲线以评估miR-195-5p对CAS的诊断潜力。采用Kaplan-Meier曲线和Cox回归来确定miR-195-5p的预后意义。体外，使用CCK-8和Transwell试验评估了miR-195-5p模拟物或抑制剂对VSMC增殖和迁移的影响.
结果：在CAS患者中，血清miR-195-5p水平升高,且与CAS程度相关。ROC曲线的AUC值为0.897,敏感性为71.4%,特异性为95.4%。较高水平的miR-195-5p表明CIE发生的风险较高，可能是CIE的独立预测因子。miR-195-5p上调促进VSMC增殖和迁移,而下调则产生了相反的效果。
结论：miR-195-5p被证明在CAS中具有诊断和预后意义，并可能作为潜在的生物标志物。它可能通过促进VSMC的增殖和迁移来促进CAS的发展。
方法：本研究涉及112名CAS患者和65名健康个体。使用RT-qPCR测量血清miR-195-5p水平。然后绘制ROC曲线以评估miR-195-5p对CAS的诊断潜力。采用Kaplan-Meier曲线和Cox回归来确定miR-195-5p的预后意义。体外，使用CCK-8和Transwell试验评估了miR-195-5p模拟物或抑制剂对VSMC增殖和迁移的影响.
结果：在CAS患者中，血清miR-195-5p水平升高,且与CAS程度相关。ROC曲线的AUC值为0.897,敏感性为71.4%,特异性为95.4%。较高水平的miR-195-5p表明CIE发生的风险较高，可能是CIE的独立预测因子。miR-195-5p上调促进VSMC增殖和迁移,而下调则产生了相反的效果。
结论：miR-195-5p被证明在CAS中具有诊断和预后意义，并可能作为潜在的生物标志物。它可能通过促进VSMC的增殖和迁移来促进CAS的发展。
