背景:石斛是兰科石斛属的多年生草本植物。一般来说,铁皮石斛(TP)和霍山石斛(HS)都被认为具有滋阴功能,而金皮石斛(JC)有较好的清热功效。然而,因为石斛种类繁多,石斛的分类和临床应用经常被混淆,在不同的药用用途中明确区分。
目的:为了比较三种石斛的差异积累代谢产物(DAMs)和差异表达基因(DEGs)。
方法:我们选择了TP,HS,和JC在石头上种植,用于2到3年的代谢组学和转录组学分析。
结果:结果表明,共获得489种代谢物,其中72个DAM主要富集在代谢途径和次生代谢产物的生物合成。转录组分析结果表明,在三种石斛中鉴定出1,038个注释的DEG。综合分析表明,三种石斛在四种主要活性成分的含量分布上存在差异:黄酮,氨基酸,生物碱,糖和酒精,其中DAM和DEGs主要富集在代谢途径和次级代谢产物的生物合成中。
结论:在这项研究中,代谢组学和转录组学用于比较三种石斛之间的差异,为今后不同药用石斛品种的研究和选择提供理论参考,为进一步研究石斛中黄酮类化合物的生物合成奠定基础。
BACKGROUND: Dendrobium is a perennial herb of the genus Dendrobium in the orchid family. Generally, Dendrobium officinale (TP) and Dendrobium huoshanense (HS) are both considered to have the function of yin-nourishing, while Dendrobium nobile (JC) has better efficacy of heat-clearing. However, because of the wide variety of Dendrobium species, the classification and clinical application of Dendrobium are often confused clearly distinguished in different medicinal uses.
OBJECTIVE: In order to compare the differentially accumulated metabolites (DAMs) and differentially expressed genes (DEGs) of the three Dendrobium.
METHODS: We selected TP, HS, and JC cultivated on stone for
metabolomic and transcriptomic analyses between 2 and 3 years.
RESULTS: The results showed that a total of 489 metabolites were obtained, including 72 were DAMs. The 72 DAMs were mainly enriched in metabolic pathways and biosynthesis of secondary metabolites. Transcriptome analysis results showed that 1,038 annotated DEGs were identified among the three Dendrobium species. The comprehensive analysis showed that the three Dendrobium differed in the distribution of the content of four major active components: flavonoids, amino acids, alkaloids, and sugars and alcohols, among which the DAMs and DEGs were mainly enriched in metabolic pathways and secondary metabolite biosynthesis.
CONCLUSIONS: In this study, metabolomics and transcriptomics were utilized to compare the differences among the three species of Dendrobium, to provide theoretical references for future research and selection of different species of Dendrobium based on different medicinal uses, and to lay the foundation for further research on the biosynthesis of flavonoids in Dendrobium.