■为了在体内进行比较,在已建立的角叉菜胶诱导的炎症的后爪模型以及发炎的颞下颌关节中,源自人脐血管周围间充质细胞的裂解物与细胞本身的急性抗炎作用。
■收获人脐带血管周围细胞并在无异种和无血清条件下培养至P3。此外,使用P3细胞制备专有的0.22微米过滤裂解物。首先,CD1免疫活性小鼠接受角叉菜胶的单侧后爪注射诱导炎症,随后立即用盐水治疗(阴性对照),1%细胞裂解物,或活细胞。对侧爪保持未注射角叉菜胶。在注射前和48小时后测量爪围,处死后在切除的组织中测量髓过氧化物酶和TNF-α浓度。第二,具有免疫能力的雄性Wistar大鼠接受来自相同治疗组的单侧颞下颌关节内(TMJ)注射,并在注射后4和48小时处死。对侧TMJ保持未注射角叉菜胶。关节组织和滑膜抽吸物,从治疗的TMJ获得组织学和白细胞浸润分析。
■裂解物和细胞处理的后爪显示组织水肿减少,并且与未处理的对照相比,在48小时时显著降低髓过氧化物酶和TNF-α的浓度。与对照组和组织学证据相比,经治疗的TMJ显示滑膜中白细胞的浓度较低,在关节周围组织中,减少炎症。
■在这项初步研究中,人脐血管周围细胞和由其产生的高度稀释的裂解物均具有抗炎作用。
UNASSIGNED: To compare in vivo, the acute anti-inflammatory effects of a lysate derived from human umbilical perivascular mesenchymal cells with the cells themselves in both an established hind-paw model of carrageenan-induced inflammation and also in the inflamed temporomandibular joint.
UNASSIGNED: Human umbilical cord perivascular cells were harvested and cultured in xeno- and serum-free conditions to P3. In addition, P3 cells were used to prepare a proprietary 0.22 micron filtered lysate. First, CD1 immunocompetent mice underwent unilateral hind-paw injections of carrageenan for induction of inflammation, followed immediately by treatment with saline (negative control), 1% cell lysate, or viable cells. The contralateral paw remained un-injected with carrageenan. Paw circumference was measured prior to injections and 48 hr later and myeloperoxidase and TNF-alpha concentrations were measured post-sacrifice in excised tissue. Second, immunocompetent Male Wistar rats underwent unilateral intra-articular temporomandibular (TMJ) injections from the same treatment groups and were sacrificed at 4 and 48 hr post-injection. The contralateral TMJ remained un-injected with carrageenan. Articular tissue and synovial aspirates, from the treated TMJ were obtained for histologic and leukocyte infiltration analyses.
UNASSIGNED: The lysate and cell-treated hind-paw demonstrated reduced tissue edema, and significantly lower concentrations of myeloperoxidase and TNF-alpha at 48 hr compared to untreated controls. Treated TMJs demonstrated lower concentrations of leukocytes in the synovium compared to controls and histologic evidence, in the peri-articular tissue, of reduced inflammation.
UNASSIGNED: In this preliminary study, both the human umbilical perivascular cells and a highly diluted lysate produced therefrom were anti-inflammatory.