■激活NLRP3(NOD-,LRR-,和含pyrin结构域的蛋白3)在阿尔茨海默病(AD)的发病机理中至关重要。多模式治疗干预是改变AD进展过程的最可行方法。因此,本研究旨在研究甜菜碱(BET)和初榨椰子油(VCO)对氯化铝诱导的Wistar大鼠AD中NLRP3的调节。
■分别口服给予BET(100,200mg/kg)和VCO(1,5g/kg)和组合(BET100mg/kg+VCO1g/kg和BET200mg/kg+VCO5g/kg)42天。在第21天和第42天,进行行为测试以检查动物的认知。乙酰胆碱酯酶(AChE)活性,氧化应激标志物,NLRP3和IL-1β的估算,在海马(H)和皮质(C)进行组织学检查。
■用BET和VCO单独或联合治疗改善了行为特征(MWM和PAp<0.0001;EPMp=0.5184),抑制AChE活性(C,p=0.0101;H,p<0.0001),并降低了大脑中的氧化应激。此外,联合治疗恢复了NLRP3水平(C,p=0.0062;H,p<0.0001)和IL1β(C,p=0.0005;H,p=0.0098)。联合治疗明显减轻了神经元变性的程度,淀粉样蛋白沉积,脑组织坏死.目前的研究表明,该组合策略通过调节NLRP3炎性体通路有效地控制了神经炎症,为新的治疗方法铺路。
UNASSIGNED: Activating NLRP3 (NOD-, LRR-, and pyrin domain-containing protein 3) is crucial in the pathogenesis of Alzheimer\'s disease (AD). A multimodal treatment intervention is the most feasible way to alter the course of AD progression. Hence, the current study was conducted to study the combination of betanin (BET) and virgin coconut oil (VCO) on NLRP3 regulation in aluminum chloride-induced AD in Wistar rats.
UNASSIGNED: BET (100,200 mg/kg) and VCO (1, 5 g/kg) alone and in combination (BET 100 mg/kg + VCO 1 g/kg and BET 200 mg/kg + VCO 5 g/kg) were given orally for 42 days. On day 21 and 42nd, the behavioral test was performed to check the animal\'s cognition. Acetylcholinesterase (AChE) activity, oxidative stress markers, estimation of NLRP3 and IL-1β, and histological examinations were conducted in the hippocampus (H) and cortex (C).
UNASSIGNED: Treatment with BET and VCO alone or combined improved behavioral characteristics (MWM and PA p < 0.0001; EPM p = 0.5184), inhibited AChE activity (C, p = 0.0101; H, p < 0.0001), and lowered oxidative stress in the brain. Also, combination treatment restored the levels of NLRP3 (C, p = 0.0062; H, p < 0.0001) and IL1β (C, p = 0.0005; H, p = 0.0098). The combination treatment significantly reduced the degree of neuronal degeneration, amyloid deposition, and necrosis in the brain tissue. The current study revealed that the combination strategy effectively controlled neuroinflammation via modulation of the NLRP3 inflammasome pathway, paving the way for the new treatment.