■三阴性乳腺癌(TNBC)由于炎症信号通路失调和肿瘤微环境的显着变化而具有高复发率,可能会影响几种疗法的失败。半胱氨酰白三烯受体1(CYSLTR1),一种炎症的白三烯调节剂,已被证明在癌症的发病机制和生存中起重要作用,但很少有研究报道其在乳腺癌中的作用。
目前的工作是使用具有组学数据的公开平台进行的,以评估CYSLTR1表达的临床潜力及其在乳腺癌患者的大型样本队列中的预后验证。包含临床信息的网络平台,选择RNA-seq和蛋白质数据以进行潜在标记CYLSTR1的计算机模拟分析。加在一起,这些平台包括相关模块,表达式,预后,药物相互作用,和基因网络的构建。
■Kaplan-Meier曲线显示,在基础亚型中,CYSLTR1水平降低对应于总体生存(p<0.005)和无复发生存(p<0.001)的不利结果。此外,与邻近的健康组织相比,CYSLTR1在乳腺肿瘤样品中下调(p<0.01),并且相对于其他亚型,基底亚型表现出最低的CYSLTR1表达(p<0.0001)。此外,基因网络分析显示,当在TNBC数据集上进行测试时,CYSLTR1与两个蛋白质编码基因(P2RY10和XCR1)具有很强的相关性.
■我们的数据强调了CYSLTR1的相关性,因为它可能在TNBC治疗中起重要作用。然而,进一步的体外和体内研究应旨在验证我们的发现,以提高我们对TNBC病理学的理解。
UNASSIGNED: Triple negative breast cancer (TNBC) has high relapse rates due to dysregulated inflammatory signaling pathways and significant changes in the tumor microenvironment, probably influencing the failure of several therapies. The Cysteinyl Leukotriene Receptor 1 (CYSLTR1), a leukotriene modulator of inflammation, has been shown to play an important role in cancer pathogenesis and survival but few studies have been reported on its role in breast cancer.
UNASSIGNED: The present work was conducted using publicly available platforms that have omics data to assess the clinical potential of CYSLTR1 expression and its prognostic validation in large cohorts of samples from breast cancer patients. Web platforms containing clinical information, RNA-seq and protein data were selected to perform in silico analyses of the potential marker CYLSTR1. Added together, the platforms included modules for correlation, expression, prognosis, drug interactions, and construction of gene networks.
UNASSIGNED: Kaplan-Meier curves revealed that reduced levels of CYSLTR1 corresponded to an unfavorable outcome for overall survival (p<0.005) as well as relapse-free survival (p<0.001) in the basal subtype. Additionally, CYSLTR1 was downregulated in breast tumor samples compared to adjacent healthy tissue (p<0.01) and the basal subtype exhibited the lowest expression of CYSLTR1 relative to the other subtypes (p<0.0001). Furthermore, gene networking analysis showed strong associations of CYSLTR1 with two protein-coding genes (P2RY10 and XCR1) when tested on a TNBC dataset.
UNASSIGNED: Our data highlighted the relevance of CYSLTR1 since it may play an important role in TNBC therapy. However, further in vitro and in vivo studies should be directed towards validating our findings in an effort to improve our understanding of TNBC pathology.