UNASSIGNED: The study included 30 individuals with periodontitis Grade B and 19 subjects with periodontitis Grade C. Quantitative and qualitative microbiological analysis of flora in pockets ≥ 7 mm was performed for the presence of selected periopathogens of the orange, red complex and Aggregatibacter actinomycetemcomitans using real-time PCR. The concentrations of IL-1 and MMP-8 in GCF were evaluated with the ELISA method.
UNASSIGNED: There were no differences in the composition of the subgingival biofilm depending on the diagnosis. The concentration of MMP-8 in GCF was significantly higher in periodontitis Grade C than in periodontitis Grade B (61 ng/µl and 37 ng/µl respectively, p = 0.039). The concentration of IL-1β was similar in both groups. No significant correlations were observed between the occurrence of individual periopathogens and concentrations of MMP-8 and IL-1β depending on the diagnosis.
UNASSIGNED: Periodontitis grade may not be distinguished according to microbial analysis of subgingival biofilm or to concentration of IL-1β in GCF. On the other hand, higher concentrations of MMP-9 in GCF from deep pockets may be helpful in detecting subjects particularly prone to occurrence and rapid progress of periodontitis.