组织培养方法可用于评估各种应力的耐受性,因为在体外条件下容易控制应力。本研究以甘蔗愈伤组织为模型系统,探讨甘蔗基因型对干旱胁迫的响应。为了诱导甘蔗愈伤组织,Murashige和Skoog(MS)的培养基与不同的甘露醇浓度(100、200和300mM)一起使用,以测量它们对愈伤组织频率的影响,愈伤组织开始的那一天,胚胎发生潜力,相对增长率(RGR),水和脯氨酸含量,K+和Na+含量,以及三种甘蔗基因型的芽和根的形成(例如,GT54-9、G84-47和pH8013)。使用五种寡核苷酸引物进行RAPD-PCR分析,以鉴定甘蔗基因型之间的遗传变异。结果表明,愈伤组织的增殖程度在70-86%之间。愈伤组织增殖值最高,PGR,与其他两种基因型(G84-47和pH8013)相比,基因型GT54-9记录了芽形成。用100mM甘露醇处理的愈伤组织显示出最高的RGR,基因型GT54-9的脯氨酸和waer含量,而,对于基因型pH8013,用300mM处理的那些记录了这些参数的最低值。基因型G84-47中Na+含量最高,而基因型pH8013收集的K+含量最高。这项研究的结果推荐了对GT54-9基因型的偏好,这被认为是最有希望的基因型,基于所有研究的性状,显示出对干旱胁迫的耐受性。
Tissue culture methods are useful in assessing the tolerance of various stresses due to the ease of controlling stress under in vitro conditions. This study aimed to investigate the response of sugarcane genotyps to drought stress using calli as a model system. For inducing sugarcane callus, the medium of Murashige and Skoog (MS) was used with different mannitol concentrations (100, 200, and 300 mM) to measure their effects on callus frequency, the day of callus initiation, embryogenic potential, relative growth rate (RGR), water and proline contents, K+ and Na+ contents, as well as the formation of shoot and roots for three sugarcane genotypes (e.g., GT 54-9, G 84-47, and pH 8013). The RAPD-PCR analysis was carried out using five oligonucleotide primers to identify the genetic variation among sugarcane genotypes. The results indicated that the degree of callus proliferation varied from 70 - 86%. The highest value of callus proliferation, PGR, shoot formation was recorded for the genotype GT 54-9 compared to the other two genotypes (G 84-47 and pH 8013). Calli treated with 100 mM mannitol showed the highest RGR, proline and waer contents for the genotype GT 54-9, while, those treated with 300 mM recorded the lowest values of these parameters for the genotype pH 8013. The genotype G 84-47 collected highest Na+ content, while the genotype pH 8013 collected highest K+ content. The results of this study recommend preference for GT 54-9 genotype, which is considered the most promising genotype, showing more tolerance to drought stress based on all studied traits.