PDF(Pubmed)
Abstract：
Dipcadi montanum (Dalz.) Baker (Asparagaceae) is a rare scapigerous herb endemic to the Western Ghats, a global biodiversity hotspot running parallel to the western coast of India. This study reports the development of a reproducible protocol for mass propagation of this underutilized geophyte using bulb scale and immature leaf base explants. Miniature bulblets were successfully induced from both types of explants after 4 and 8 weeks of culture on full-strength semisolid MS basal medium fortified with 3% sucrose and varying levels of BAP (4.4-17.7 μM) and TDZ (4.5-18.1 μM). The addition of 2.7 μM NAA further enhanced the rate of microbulb induction. Rooting of the 8-week-old bulblets, obtained from both explants, was achieved with more than 90% efficiency on liquid as well as agar-gelled half-strength MS basal medium fortified with varying levels of IBA (2.46-9.84 μM) and NAA (2.68-10.74 μM), with or without 2.32 μM Kinetin. More than 95% of the rooted plants survived the initial acclimatization process under controlled ex-vitro conditions, and a survival rate of over 80% was recorded after 4 weeks of transfer to greenhouse conditions. After a brief dormancy, the regenerants resumed growth in the postmonsoon season and exhibited morphological resemblance to the donor plant. Comparative cytological analysis between the donor and 15 randomly selected regenerants revealed a stable somatic count of 2n = 20.
摘要：
Dipcadimontanum(Dalz.)贝克(天门冬科)是西高止山脉特有的一种罕见的替罪羊草本植物,与印度西海岸平行的全球生物多样性热点。这项研究报告了使用鳞茎鳞片和未成熟叶基部外植体对这种未充分利用的地生植物进行大规模繁殖的可重复方案的开发。在用3％蔗糖和不同水平的BAP（4.4-17.7μM）和TDZ（4.5-18.1μM）强化的全强度半固体MS基础培养基上培养4周和8周后，成功地从两种类型的外植体中诱导出微型鳞茎。加入2.7μMNAA进一步提高了微球诱导率。8周大的小鱼生根，从两个外植体获得，在用不同水平的IBA（2.46-9.84μM）和NAA（2.68-10.74μM）强化的液体和琼脂凝胶半强度MS基础培养基上，效率超过90％，含或不含2.32μM激肽。在受控的离体条件下，超过95％的生根植物在最初的驯化过程中幸存下来，转移到温室条件4周后，存活率超过80％。短暂休眠后,再生剂在季后风季节恢复生长，并表现出与供体植物的形态相似。供体和15个随机选择的再生剂之间的比较细胞学分析显示，稳定的体细胞计数为2n=20。
