目的:核仁组织区(NORs)是转录为核糖体RNA的DNA卷曲。NOR相关蛋白,称为嗜银NOR(AgNOR),通过光学显微镜用硝酸银检查染色在细胞核内可见。AgNOR计数是一种增殖标志物,可能有助于各种肿瘤病变的诊断和预后。非整倍体(DNA含量异常)可以预测进展,肿瘤的生存和预后。这项研究的目的是评估AgNORs的作用,DNA倍性状态,和总S期分数(TSPF)作为恶性唾液腺肿瘤(MSGTs)的预后参数。
方法:当前的研究是对一组MSGT(N=47)的回顾性研究,使用硝酸银染色评估AgNORs,DNA指数(DI),和TSPF使用流式细胞术(FCM)。数据包括肿瘤大小和部位,淋巴管浸润(LVI),收集淋巴结转移(LNM)。
结果:AgNORs计数与MSGT类型有统计学意义。发现DI与肿瘤部位有显著关联,肿瘤大小和MSGT类型。此外,发现TSPF与LVI显著相关。AgNORs计数与TSPF之间呈中度正相关。LNM,肿瘤部位,高AgNOR和低DI均与无病生存期(DFS)短和总生存期(OS)差相关.
结论:本研究表明,高AgNORs计数,DNA非整倍体和TSPF对MSGTs预后影响较差。
OBJECTIVE: Nucleolar organizer regions (NORs) are DNA coils that transcribe to ribosomal RNA. The NOR-associated protein, termed argyrophilic NOR (AgNOR), was visible within the nucleus by staining with silver nitrate examination via the light microscope. AgNOR counting is a proliferation marker and may help in the diagnosis and prognosis of various neoplastic lesions. Aneuploidy (abnormal DNA content) can predict the progression, survival and prognosis of the tumors. The aim of this study was to evaluate the role of AgNORs, DNA ploidy status, and total S-phase fraction (TSPF) as prognostic parameters in malignant salivary gland tumors (MSGTs).
METHODS: The current study is a retrospective study on a cohort of MSGTs (N=47), to assess AgNORs using Silver Nitrate stain, DNA index (DI), and TSPF using flow cytometry (FCM). Data including tumor size and site, lymphovascular invasion (LVI), lymph node metastasis (LNM) were collected.
RESULTS: The AgNORs count was statistically significant with MSGT type. DI was found to have a significant association with tumor site, tumor size and MSGT type. In addition, TSPF was found to be significantly associated with LVI. A moderate positive correlation was noted between AgNORs count and TSPF. LNM, tumor site, high AgNORs and low DI were all associated with short disease-free survival (DFS) and poor overall survival (OS).
CONCLUSIONS: The present study revealed that high AgNORs count, DNA aneuploidy and TSPF had a poor influence on MSGTs prognosis.