■肺癌是最常见的诊断和癌症死亡的主要原因,通常与吸烟有关。此外,暴露于香烟烟雾的人的微生物群可以改变,使得难以消灭机会性微生物。雪梨的叶子是水果生产的副产品,到目前为止,没有关于抗增殖的研究,抗炎,和抗菌活性。
■研究抗菌剂,一氧化氮(NO)产生抑制,的抗增殖活性及其对烟草损害的治疗和预防可能的影响。
■通过加氢蒸馏(3h)获得精油(EO)。通过GC-MS研究了其化学成分。有人建议研究对人肿瘤细胞系的抗增殖活性,即,乳腺癌(MCF-7),肺(NCI-H460),宫颈(HeLa),和肝细胞(HepG2)癌。还测试了来自猪肝的非肿瘤原代培养物(PLP2)。通过脂多糖刺激的鼠巨噬细胞系评估EO抑制一氧化氮(NO)产生的能力。针对7株细菌和8种真菌,研究了对机会性病原体的抗菌和抗真菌活性。
■结果表明精油中存在23种化合物,大多数是司他烯醇(45.63%)和β-石竹烯氧化物(12.72%)。叶EO在92.04μgmL-1的浓度下对一氧化氮的产生提供50%的抑制。EO还显示了对所有研究的人类肿瘤细胞系的抗增殖活性,GI50值介于270.86和337.25µgmL-1之间。精油显示出对细菌单核细胞增生李斯特菌的抗菌潜力(Murray等人。)Pirie(NCTC7973)和鼠伤寒沙门氏菌ATCC13311(MIC1870µgmL-1)和真菌杂色曲霉ATCC11730,曲霉ATCC12066,青霉菌ATCC90288,疣青霉var。cyclopium(Westling)Samson,Stolk&Hadlok(食品分离物)(MIC1870µgmL-1)和绿色木霉。IAM5061(1,400µgmL-1)。
■经证明的抗炎,抗增殖,和抗微生物活性在E.pyriformis的叶子可以增加价值,该植物的生产链,作为预防和对抗癌症的可能选择,包括肺癌.
UNASSIGNED: Lung cancer is the most commonly diagnosed and the main cause of cancer death, usually related to cigarette smoking. Furthermore, the microbiota of people exposed to cigarette smoke can be modified, making it difficult to eliminate opportunistic microorganisms. The leaves of Eugenia pyriformis are a by-product of fruit production and, to date, there have been no studies addressing the antiproliferative, anti-inflammatory, and antimicrobial activities.
UNASSIGNED: Investigate the antimicrobial, Nitric Oxide (NO)-production inhibition, and antiproliferative activities of the essential oil from E. pyriformis leaves and its possible effect on the treatment and prevention of damage caused by tobacco.
UNASSIGNED: The essential oil (EO) was obtained by hydrodistillation (3 h). Its chemical composition was investigated by GC-MS. It was proposed to investigate antiproliferative activity against human tumor cell lines, namely, breast adenocarcinoma (MCF-7), lung (NCI-H460), cervical (HeLa), and hepatocellular (HepG2) carcinomas. A non-tumor primary culture from pig liver (PLP2) was also tested. The EO capacity to inhibit nitric oxide (NO) production was evaluated by a lipopolysaccharide stimulated murine macrophage cell line. Antibacterial and antifungal activities against opportunistic pathogens were investigated against seven strains of bacteria and eight fungi.
UNASSIGNED: The results indicated the presence of 23 compounds in the essential oil, the majority were spathulenol (45.63%) and β-caryophyllene oxide (12.72%). Leaf EO provided 50% inhibition of nitric oxide production at a concentration of 92.04 µg mL-1. The EO also demonstrated antiproliferative activity against all human tumor cell lines studied, with GI50 values comprised between 270.86 and 337.25 µg mL-1. The essential oil showed antimicrobial potential against the bacteria Listeria monocytogenes (Murray et al.) Pirie (NCTC 7973) and Salmonella Typhimurium ATCC 13311 (MIC 1870 µg mL-1) and fungi Aspergillus versicolor ATCC 11730, Aspergillus ochraceus ATCC 12066, Penicillium ochrochloron ATCC 90288, Penicillium verrucosum var. cyclopium (Westling) Samson, Stolk & Hadlok (food isolate) (MIC 1870 µg mL-1) and Trichoderma viride Pers. IAM 5061 (1,400 µg mL-1).
UNASSIGNED: The demonstrated anti-inflammatory, antiproliferative, and antimicrobial activities in the leaves of E. pyriformis can add value to the production chain of this plant, being a possible option for preventing and combating cancer, including lung cancer.